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Decreased astroglial cell adhesion and proliferation on zinc oxide nanoparticle polyurethane composites.

Seil JT, Webster TJ - Int J Nanomedicine (2008)

Bottom Line: The surface chemistry was characterized via X-ray photoelectron spectroscopy.Astrocyte adhesion was significantly reduced on ZnO nanoparticle/polyurethane (PU) composites with a weight ratio of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% in comparison to pure PU.The successful production of ZnO nanoparticle composite scaffolds suitable for decreasing astroglial cell density demonstrates their potential as a nerve guidance channel material with greater efficiency than what may be available today.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Nanomedicine Research, Division of Engineering, Brown University, Providence, RI 02912, USA.

ABSTRACT
Nanomaterials offer a number of properties that are of interest to the field of neural tissue engineering. Specifically, materials that exhibit nanoscale surface dimensions have been shown to promote neuron function while simultaneously minimizing the activity of cells such as astrocytes that inhibit central nervous system regeneration. Studies demonstrating enhanced neural tissue regeneration in electrical fields through the use of conductive materials have led to interest in piezoelectric materials (or those materials which generate a transient electrical potential when mechanically deformed) such as zinc oxide (ZnO). It has been speculated that ZnO nanoparticles possess increased piezoelectric properties over ZnO micron particles. Due to this promise in neural applications, the objective of the present in vitro study was, for the first time, to assess the activity of astroglial cells on ZnO nanoparticle polymer composites. ZnO nanoparticles embedded in polyurethane were analyzed via scanning electron microscopy to evaluate nanoscale surface features of the composites. The surface chemistry was characterized via X-ray photoelectron spectroscopy. Astroglial cell response was evaluated based on cell adhesion and proliferation. Astrocyte adhesion was significantly reduced on ZnO nanoparticle/polyurethane (PU) composites with a weight ratio of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% in comparison to pure PU. The successful production of ZnO nanoparticle composite scaffolds suitable for decreasing astroglial cell density demonstrates their potential as a nerve guidance channel material with greater efficiency than what may be available today.

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Surface energy of ZnO/PU composites. Surface energy was calculated for each sample by measuring the contact angle of three liquids at the sample surface and entering values into the Owens-Wendt equation.Notes: Values are mean ± SEM; n = 3; *p < 0.05 (compared to 100:0 [PU:ZnO] wt.%), **p < 0.05 (compared to 90:10 [PU:ZnO] wt.%).
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f2-ijn-3-523: Surface energy of ZnO/PU composites. Surface energy was calculated for each sample by measuring the contact angle of three liquids at the sample surface and entering values into the Owens-Wendt equation.Notes: Values are mean ± SEM; n = 3; *p < 0.05 (compared to 100:0 [PU:ZnO] wt.%), **p < 0.05 (compared to 90:10 [PU:ZnO] wt.%).

Mentions: Surface energy calculations from contact angle data indicated that adding ZnO nanoparticles to PU increased sample surface energy. Samples with weight ratios of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, 90:10 (PU:ZnO) wt.% and 98:2 (PU:ZnO) wt.% had a surface energy significantly higher than the pure polymer (Figure 2). These samples had a lower contact angle for each liquid as the ZnO nanoparticle concentration increased (Table 2). Incorporation of ZnO nanoparticles significantly increased surface energy and wettability of PU at all nanoparticle concentrations. For samples with weight ratios of 50:50 (PU:ZnO) wt.% and 75:25 (PU:ZnO) wt.%, surface energy was significantly higher in comparison to samples with a weight ratio of 90:10 (PU:ZnO) wt.%.


Decreased astroglial cell adhesion and proliferation on zinc oxide nanoparticle polyurethane composites.

Seil JT, Webster TJ - Int J Nanomedicine (2008)

Surface energy of ZnO/PU composites. Surface energy was calculated for each sample by measuring the contact angle of three liquids at the sample surface and entering values into the Owens-Wendt equation.Notes: Values are mean ± SEM; n = 3; *p < 0.05 (compared to 100:0 [PU:ZnO] wt.%), **p < 0.05 (compared to 90:10 [PU:ZnO] wt.%).
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2636581&req=5

f2-ijn-3-523: Surface energy of ZnO/PU composites. Surface energy was calculated for each sample by measuring the contact angle of three liquids at the sample surface and entering values into the Owens-Wendt equation.Notes: Values are mean ± SEM; n = 3; *p < 0.05 (compared to 100:0 [PU:ZnO] wt.%), **p < 0.05 (compared to 90:10 [PU:ZnO] wt.%).
Mentions: Surface energy calculations from contact angle data indicated that adding ZnO nanoparticles to PU increased sample surface energy. Samples with weight ratios of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, 90:10 (PU:ZnO) wt.% and 98:2 (PU:ZnO) wt.% had a surface energy significantly higher than the pure polymer (Figure 2). These samples had a lower contact angle for each liquid as the ZnO nanoparticle concentration increased (Table 2). Incorporation of ZnO nanoparticles significantly increased surface energy and wettability of PU at all nanoparticle concentrations. For samples with weight ratios of 50:50 (PU:ZnO) wt.% and 75:25 (PU:ZnO) wt.%, surface energy was significantly higher in comparison to samples with a weight ratio of 90:10 (PU:ZnO) wt.%.

Bottom Line: The surface chemistry was characterized via X-ray photoelectron spectroscopy.Astrocyte adhesion was significantly reduced on ZnO nanoparticle/polyurethane (PU) composites with a weight ratio of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% in comparison to pure PU.The successful production of ZnO nanoparticle composite scaffolds suitable for decreasing astroglial cell density demonstrates their potential as a nerve guidance channel material with greater efficiency than what may be available today.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Nanomedicine Research, Division of Engineering, Brown University, Providence, RI 02912, USA.

ABSTRACT
Nanomaterials offer a number of properties that are of interest to the field of neural tissue engineering. Specifically, materials that exhibit nanoscale surface dimensions have been shown to promote neuron function while simultaneously minimizing the activity of cells such as astrocytes that inhibit central nervous system regeneration. Studies demonstrating enhanced neural tissue regeneration in electrical fields through the use of conductive materials have led to interest in piezoelectric materials (or those materials which generate a transient electrical potential when mechanically deformed) such as zinc oxide (ZnO). It has been speculated that ZnO nanoparticles possess increased piezoelectric properties over ZnO micron particles. Due to this promise in neural applications, the objective of the present in vitro study was, for the first time, to assess the activity of astroglial cells on ZnO nanoparticle polymer composites. ZnO nanoparticles embedded in polyurethane were analyzed via scanning electron microscopy to evaluate nanoscale surface features of the composites. The surface chemistry was characterized via X-ray photoelectron spectroscopy. Astroglial cell response was evaluated based on cell adhesion and proliferation. Astrocyte adhesion was significantly reduced on ZnO nanoparticle/polyurethane (PU) composites with a weight ratio of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% in comparison to pure PU. The successful production of ZnO nanoparticle composite scaffolds suitable for decreasing astroglial cell density demonstrates their potential as a nerve guidance channel material with greater efficiency than what may be available today.

Show MeSH
Related in: MedlinePlus