Limits...
Aberrant p63 and WT-1 expression in myoepithelial cells of pregnancy-associated breast cancer: implications for tumor aggressiveness and invasiveness.

Xu Z, Wang W, Deng CX, Man YG - Int. J. Biol. Sci. (2009)

Bottom Line: Hyperplastic cells with cytoplasmic p63 expression often adjacent to, and share a similar immunohistochemical and cytological profile with, invasive cancer cells.To our best knowledge, our main finings have not been previously reported.Our findings suggest that the functional status of myoepithelial cells may be significantly associated with tumor aggressiveness and invasiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Thyroid and Breast Surgery, China-Japan Union Hospital, Jilin University, Changchun, Jilin, China.

ABSTRACT
Our recent studies revealed that focal alterations in breast myoepithelial cell layers significantly impact the biological presentation of associated epithelial cells. As pregnancy-associated breast cancer (PABC) has a significantly more aggressive clinical course and mortality rate than other forms of breast malignancies, our current study compared tumor suppressor expression in myoepithelial cells of PABC and non-PABC, to determine whether myoepithelial cells of PABC may have aberrant expression of tumor suppressors. Tissue sections from 20 cases of PABC and 20 cases of stage, grade, and age matched non-PABC were subjected to immunohistochemistry, and the expression of tumor suppressor maspin, p63, and Wilms' tumor 1 (WT-1) in calponin positive myoepithelial cells were statistically compared. The expression profiles of maspin, p63, and WT-1 in myoepithelial cells of all ducts encountered were similar between PABC and non-PABC. PABC, however, displayed several unique alterations in terminal duct and lobular units (TDLU), acini, and associated tumor tissues that were not seen in those of non-PABC, which included the absence of p63 and WT-1 expression in a vast majority of the myoepithelial cells, cytoplasmic localization of p63 in the entire epithelial cell population of some lobules, and substantially increasing WT-1 expression in vascular structures of the invasive cancer component. All or nearly all epithelial cells with aberrant p63 and WT-1 expression lacked the expression of estrogen receptor and progesterone receptor, whereas they had a substantially higher proliferation index than their counterparts with p63 and WT-1 expression. Hyperplastic cells with cytoplasmic p63 expression often adjacent to, and share a similar immunohistochemical and cytological profile with, invasive cancer cells. To our best knowledge, our main finings have not been previously reported. Our findings suggest that the functional status of myoepithelial cells may be significantly associated with tumor aggressiveness and invasiveness.

Show MeSH

Related in: MedlinePlus

Reduced p63 and WT-1 expression in calponin and maspin positive myoepithelial cells of PABC. Two sets of four adjacent sections were immunostained for calponin and mapin, p63, and WT-1. Asterisks identify normal ducts in the inter-lobular space. Arrows identify myoepithelial cell layers. Note that a vast majority of the myoepithelial cells in normal ducts are positive for all four markers, whereas a vast majority of the myoepithelial cells in the acini lack p63 and WT-1 expression. 200X.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2631157&req=5

Figure 3: Reduced p63 and WT-1 expression in calponin and maspin positive myoepithelial cells of PABC. Two sets of four adjacent sections were immunostained for calponin and mapin, p63, and WT-1. Asterisks identify normal ducts in the inter-lobular space. Arrows identify myoepithelial cell layers. Note that a vast majority of the myoepithelial cells in normal ducts are positive for all four markers, whereas a vast majority of the myoepithelial cells in the acini lack p63 and WT-1 expression. 200X.

Mentions: In sets of four immediate consecutive sections, calponin and maspin were uniformly expressed in all or nearly all morphologically distinct myoepithelial cells (Fig. 3a-3b). In sharp contrast, in about 80% of the acini and TDLU, only about 10% and 30% of calponin positive myoepithelial cells showed WT-1 and p63 expression, respectively (Figs. 3-4; Table 1). Under high magnification, all or nearly all acini and TDLU with reduced p63 and WT-1 expression showed fragmentations or small focal disruptions in the surrounding myoepithelial cell layers. In about 20% of the acini and TDLU, however, all or nearly all morphologically distinct myoepithelial cells uniformly expressed all four markers. These acini and TDLU were arranged as morphologically distinct lobules with variable sizes. These lobules were generally separated by stromal tissues from adjacent lobules that showed significantly reduced p63 and WT-1 expression (data not shown).


Aberrant p63 and WT-1 expression in myoepithelial cells of pregnancy-associated breast cancer: implications for tumor aggressiveness and invasiveness.

Xu Z, Wang W, Deng CX, Man YG - Int. J. Biol. Sci. (2009)

Reduced p63 and WT-1 expression in calponin and maspin positive myoepithelial cells of PABC. Two sets of four adjacent sections were immunostained for calponin and mapin, p63, and WT-1. Asterisks identify normal ducts in the inter-lobular space. Arrows identify myoepithelial cell layers. Note that a vast majority of the myoepithelial cells in normal ducts are positive for all four markers, whereas a vast majority of the myoepithelial cells in the acini lack p63 and WT-1 expression. 200X.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2631157&req=5

Figure 3: Reduced p63 and WT-1 expression in calponin and maspin positive myoepithelial cells of PABC. Two sets of four adjacent sections were immunostained for calponin and mapin, p63, and WT-1. Asterisks identify normal ducts in the inter-lobular space. Arrows identify myoepithelial cell layers. Note that a vast majority of the myoepithelial cells in normal ducts are positive for all four markers, whereas a vast majority of the myoepithelial cells in the acini lack p63 and WT-1 expression. 200X.
Mentions: In sets of four immediate consecutive sections, calponin and maspin were uniformly expressed in all or nearly all morphologically distinct myoepithelial cells (Fig. 3a-3b). In sharp contrast, in about 80% of the acini and TDLU, only about 10% and 30% of calponin positive myoepithelial cells showed WT-1 and p63 expression, respectively (Figs. 3-4; Table 1). Under high magnification, all or nearly all acini and TDLU with reduced p63 and WT-1 expression showed fragmentations or small focal disruptions in the surrounding myoepithelial cell layers. In about 20% of the acini and TDLU, however, all or nearly all morphologically distinct myoepithelial cells uniformly expressed all four markers. These acini and TDLU were arranged as morphologically distinct lobules with variable sizes. These lobules were generally separated by stromal tissues from adjacent lobules that showed significantly reduced p63 and WT-1 expression (data not shown).

Bottom Line: Hyperplastic cells with cytoplasmic p63 expression often adjacent to, and share a similar immunohistochemical and cytological profile with, invasive cancer cells.To our best knowledge, our main finings have not been previously reported.Our findings suggest that the functional status of myoepithelial cells may be significantly associated with tumor aggressiveness and invasiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Thyroid and Breast Surgery, China-Japan Union Hospital, Jilin University, Changchun, Jilin, China.

ABSTRACT
Our recent studies revealed that focal alterations in breast myoepithelial cell layers significantly impact the biological presentation of associated epithelial cells. As pregnancy-associated breast cancer (PABC) has a significantly more aggressive clinical course and mortality rate than other forms of breast malignancies, our current study compared tumor suppressor expression in myoepithelial cells of PABC and non-PABC, to determine whether myoepithelial cells of PABC may have aberrant expression of tumor suppressors. Tissue sections from 20 cases of PABC and 20 cases of stage, grade, and age matched non-PABC were subjected to immunohistochemistry, and the expression of tumor suppressor maspin, p63, and Wilms' tumor 1 (WT-1) in calponin positive myoepithelial cells were statistically compared. The expression profiles of maspin, p63, and WT-1 in myoepithelial cells of all ducts encountered were similar between PABC and non-PABC. PABC, however, displayed several unique alterations in terminal duct and lobular units (TDLU), acini, and associated tumor tissues that were not seen in those of non-PABC, which included the absence of p63 and WT-1 expression in a vast majority of the myoepithelial cells, cytoplasmic localization of p63 in the entire epithelial cell population of some lobules, and substantially increasing WT-1 expression in vascular structures of the invasive cancer component. All or nearly all epithelial cells with aberrant p63 and WT-1 expression lacked the expression of estrogen receptor and progesterone receptor, whereas they had a substantially higher proliferation index than their counterparts with p63 and WT-1 expression. Hyperplastic cells with cytoplasmic p63 expression often adjacent to, and share a similar immunohistochemical and cytological profile with, invasive cancer cells. To our best knowledge, our main finings have not been previously reported. Our findings suggest that the functional status of myoepithelial cells may be significantly associated with tumor aggressiveness and invasiveness.

Show MeSH
Related in: MedlinePlus