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Object-place recognition learning triggers rapid induction of plasticity-related immediate early genes and synaptic proteins in the rat dentate gyrus.

Soulé J, Penke Z, Kanhema T, Alme MN, Laroche S, Bramham CR - Neural Plast. (2009)

Bottom Line: Here, we examined expression of the plasticity-associated immediate early genes (Arc, Zif268, and Narp) in the dentate gyrus following long-term object-place recognition learning in rats.RT-PCR analysis from dentate gyrus tissue collected shortly after training did not reveal learning-specific changes in Arc mRNA expression.Thus, object-place recognition triggers rapid, blade-specific upregulation of plasticity-associated immediate early genes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedicine and Bergen Mental Health Research Center, University of Bergen, Bergen, Norway.

ABSTRACT
Long-term recognition memory requires protein synthesis, but little is known about the coordinate regulation of specific genes. Here, we examined expression of the plasticity-associated immediate early genes (Arc, Zif268, and Narp) in the dentate gyrus following long-term object-place recognition learning in rats. RT-PCR analysis from dentate gyrus tissue collected shortly after training did not reveal learning-specific changes in Arc mRNA expression. In situ hybridization and immunohistochemistry were therefore used to assess possible sparse effects on gene expression. Learning about objects increased the density of granule cells expressing Arc, and to a lesser extent Narp, specifically in the dorsal blade of the dentate gyrus, while Zif268 expression was elevated across both blades. Thus, object-place recognition triggers rapid, blade-specific upregulation of plasticity-associated immediate early genes. Furthermore, Western blot analysis of dentate gyrus homogenates demonstrated concomitant upregulation of three postsynaptic density proteins (Arc, PSD-95, and alpha-CaMKII) with key roles in long-term synaptic plasticity and long-term memory.

Show MeSH
Expression levels of Arc in the dentate gyrusafter object recognition. Fold change in mRNA levels (relative to the CCgroup) is presented for Arc in the dentate gyrus of animals from all fivegroups (n = 8 for all groups, except L60, n = 7). Data are presented as mean ± SEM.Gene expression was normalized to control genes (see methods).
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fig2: Expression levels of Arc in the dentate gyrusafter object recognition. Fold change in mRNA levels (relative to the CCgroup) is presented for Arc in the dentate gyrus of animals from all fivegroups (n = 8 for all groups, except L60, n = 7). Data are presented as mean ± SEM.Gene expression was normalized to control genes (see methods).

Mentions: We hypothesized that acquisition of different types of information aboutthe objects and their spatial location would be associated with rapid inductionof the immediate early gene Arc. Thisissue was first addressed by semiquantitative RT-PCR analysis of Arc mRNAlevels in the microdissected DG. Surprisingly, no significant change in Arc mRNA levels could be observedin C10, C60, L10, and L60 animals when compared with caged control (CC) animals(Figure 2, P > .05), indicating that Arc expression in the dentategyrus was not significantly affected by exploration of the arena with or withoutthe objects.


Object-place recognition learning triggers rapid induction of plasticity-related immediate early genes and synaptic proteins in the rat dentate gyrus.

Soulé J, Penke Z, Kanhema T, Alme MN, Laroche S, Bramham CR - Neural Plast. (2009)

Expression levels of Arc in the dentate gyrusafter object recognition. Fold change in mRNA levels (relative to the CCgroup) is presented for Arc in the dentate gyrus of animals from all fivegroups (n = 8 for all groups, except L60, n = 7). Data are presented as mean ± SEM.Gene expression was normalized to control genes (see methods).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2631155&req=5

fig2: Expression levels of Arc in the dentate gyrusafter object recognition. Fold change in mRNA levels (relative to the CCgroup) is presented for Arc in the dentate gyrus of animals from all fivegroups (n = 8 for all groups, except L60, n = 7). Data are presented as mean ± SEM.Gene expression was normalized to control genes (see methods).
Mentions: We hypothesized that acquisition of different types of information aboutthe objects and their spatial location would be associated with rapid inductionof the immediate early gene Arc. Thisissue was first addressed by semiquantitative RT-PCR analysis of Arc mRNAlevels in the microdissected DG. Surprisingly, no significant change in Arc mRNA levels could be observedin C10, C60, L10, and L60 animals when compared with caged control (CC) animals(Figure 2, P > .05), indicating that Arc expression in the dentategyrus was not significantly affected by exploration of the arena with or withoutthe objects.

Bottom Line: Here, we examined expression of the plasticity-associated immediate early genes (Arc, Zif268, and Narp) in the dentate gyrus following long-term object-place recognition learning in rats.RT-PCR analysis from dentate gyrus tissue collected shortly after training did not reveal learning-specific changes in Arc mRNA expression.Thus, object-place recognition triggers rapid, blade-specific upregulation of plasticity-associated immediate early genes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedicine and Bergen Mental Health Research Center, University of Bergen, Bergen, Norway.

ABSTRACT
Long-term recognition memory requires protein synthesis, but little is known about the coordinate regulation of specific genes. Here, we examined expression of the plasticity-associated immediate early genes (Arc, Zif268, and Narp) in the dentate gyrus following long-term object-place recognition learning in rats. RT-PCR analysis from dentate gyrus tissue collected shortly after training did not reveal learning-specific changes in Arc mRNA expression. In situ hybridization and immunohistochemistry were therefore used to assess possible sparse effects on gene expression. Learning about objects increased the density of granule cells expressing Arc, and to a lesser extent Narp, specifically in the dorsal blade of the dentate gyrus, while Zif268 expression was elevated across both blades. Thus, object-place recognition triggers rapid, blade-specific upregulation of plasticity-associated immediate early genes. Furthermore, Western blot analysis of dentate gyrus homogenates demonstrated concomitant upregulation of three postsynaptic density proteins (Arc, PSD-95, and alpha-CaMKII) with key roles in long-term synaptic plasticity and long-term memory.

Show MeSH