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Extracts from Cladiella australis, Clavularia viridis and Klyxum simplex (soft corals) are capable of inhibiting the growth of human oral squamous cell carcinoma cells.

Liang CH, Wang GH, Liaw CC, Lee MF, Wang SH, Cheng DL, Chou TH - Mar Drugs (2008)

Bottom Line: The morphological alterations in SCCs cells after treatment with three extracts, such as typical nuclear condensation, nuclear fragmentation and apoptotic bodies of cells were demonstrated by Hoechst stain.Flow cytometry indicated that three extracts sensitized SCC25 cells in the G(0)/G(1) and S-G(2)/M phases with a concomitant significantly increased sub-G(1) fraction, indicating cell death by apoptosis.This apoptosis process was accompanied by activation of caspase-3 expression after SCC25 cells were treated with three extracts.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan.

ABSTRACT
Many biomedical products have already been obtained from marine organisms. In order to search more therapeutic drugs against cancer, this study demonstrates the cytotoxicity effects of Cladiella australis, Clavularia viridis and Klyxum simplex extracts on human oral squamous cell carcinoma (SCC4, SCC9 and SCC25) cells using cell adhesion and cell viability assay. The morphological alterations in SCCs cells after treatment with three extracts, such as typical nuclear condensation, nuclear fragmentation and apoptotic bodies of cells were demonstrated by Hoechst stain. Flow cytometry indicated that three extracts sensitized SCC25 cells in the G(0)/G(1) and S-G(2)/M phases with a concomitant significantly increased sub-G(1) fraction, indicating cell death by apoptosis. This apoptosis process was accompanied by activation of caspase-3 expression after SCC25 cells were treated with three extracts. Thereby, it is possible that extracts of C. australis, C. viridis and K. simplex cause apoptosis of SCCs and warrant further research investigating the possible anti-oral cancer compounds in these soft corals.

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Related in: MedlinePlus

Effects of C. australis, C. viridis and K. simplex extracts on the cell cycles of SCC25 cells. Flow cytometric analysis of the cell cycle of SCC25 cells after three extracts (IC50) treatment for 18 h. (B) The percentage of cell population in the cell cycle of SCC25 cells after three extracts (IC50) treatments for 18 h. The cell populations were determined by WinMDI software. Data are means ± SD from three independent experiments.
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f3-md-06-00595: Effects of C. australis, C. viridis and K. simplex extracts on the cell cycles of SCC25 cells. Flow cytometric analysis of the cell cycle of SCC25 cells after three extracts (IC50) treatment for 18 h. (B) The percentage of cell population in the cell cycle of SCC25 cells after three extracts (IC50) treatments for 18 h. The cell populations were determined by WinMDI software. Data are means ± SD from three independent experiments.

Mentions: To confirm that C. australis, C. viridis and K. simplex extracts mediate SCC25 cell apoptosis, the cell cycle distribution and specific DNA content in the sub-G1 peak were further investigated with flow cytometric analysis. Treatment of cells with IC50 concentration of the C. australis, C. viridis and K. simplex extracts suggests that the main characteristic of apoptosis is the cleavage of nuclear DNA into multiple fragments and causing increase in the sub-G1 phase (Figure 3A). Incubation with the three extracts showed a typical pattern of DNA content that reflected in the percentage of G0/G1 and S-G2/M phases of the cell cycle together with a marked apoptotic sub-G1 phase in SCC25 cells as listed in Figure 3B.


Extracts from Cladiella australis, Clavularia viridis and Klyxum simplex (soft corals) are capable of inhibiting the growth of human oral squamous cell carcinoma cells.

Liang CH, Wang GH, Liaw CC, Lee MF, Wang SH, Cheng DL, Chou TH - Mar Drugs (2008)

Effects of C. australis, C. viridis and K. simplex extracts on the cell cycles of SCC25 cells. Flow cytometric analysis of the cell cycle of SCC25 cells after three extracts (IC50) treatment for 18 h. (B) The percentage of cell population in the cell cycle of SCC25 cells after three extracts (IC50) treatments for 18 h. The cell populations were determined by WinMDI software. Data are means ± SD from three independent experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2630851&req=5

f3-md-06-00595: Effects of C. australis, C. viridis and K. simplex extracts on the cell cycles of SCC25 cells. Flow cytometric analysis of the cell cycle of SCC25 cells after three extracts (IC50) treatment for 18 h. (B) The percentage of cell population in the cell cycle of SCC25 cells after three extracts (IC50) treatments for 18 h. The cell populations were determined by WinMDI software. Data are means ± SD from three independent experiments.
Mentions: To confirm that C. australis, C. viridis and K. simplex extracts mediate SCC25 cell apoptosis, the cell cycle distribution and specific DNA content in the sub-G1 peak were further investigated with flow cytometric analysis. Treatment of cells with IC50 concentration of the C. australis, C. viridis and K. simplex extracts suggests that the main characteristic of apoptosis is the cleavage of nuclear DNA into multiple fragments and causing increase in the sub-G1 phase (Figure 3A). Incubation with the three extracts showed a typical pattern of DNA content that reflected in the percentage of G0/G1 and S-G2/M phases of the cell cycle together with a marked apoptotic sub-G1 phase in SCC25 cells as listed in Figure 3B.

Bottom Line: The morphological alterations in SCCs cells after treatment with three extracts, such as typical nuclear condensation, nuclear fragmentation and apoptotic bodies of cells were demonstrated by Hoechst stain.Flow cytometry indicated that three extracts sensitized SCC25 cells in the G(0)/G(1) and S-G(2)/M phases with a concomitant significantly increased sub-G(1) fraction, indicating cell death by apoptosis.This apoptosis process was accompanied by activation of caspase-3 expression after SCC25 cells were treated with three extracts.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan.

ABSTRACT
Many biomedical products have already been obtained from marine organisms. In order to search more therapeutic drugs against cancer, this study demonstrates the cytotoxicity effects of Cladiella australis, Clavularia viridis and Klyxum simplex extracts on human oral squamous cell carcinoma (SCC4, SCC9 and SCC25) cells using cell adhesion and cell viability assay. The morphological alterations in SCCs cells after treatment with three extracts, such as typical nuclear condensation, nuclear fragmentation and apoptotic bodies of cells were demonstrated by Hoechst stain. Flow cytometry indicated that three extracts sensitized SCC25 cells in the G(0)/G(1) and S-G(2)/M phases with a concomitant significantly increased sub-G(1) fraction, indicating cell death by apoptosis. This apoptosis process was accompanied by activation of caspase-3 expression after SCC25 cells were treated with three extracts. Thereby, it is possible that extracts of C. australis, C. viridis and K. simplex cause apoptosis of SCCs and warrant further research investigating the possible anti-oral cancer compounds in these soft corals.

Show MeSH
Related in: MedlinePlus