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Detection of diarrheic shellfish poisoning and azaspiracid toxins in Moroccan mussels: comparison of the LC-MS method with the commercial immunoassay kit.

Elgarch A, Vale P, Rifai S, Fassouane A - Mar Drugs (2008)

Bottom Line: The LC-MS method was found to be able to detect a high level of okadaic acid (OA), low level of dinophysistoxin-2 (DTX2), and surprisingly, traces of azaspiracids 2 (AZA2) in mussels.The "DSP-Check" kit was found to detect quantitatively DSP toxins in all contaminated samples containing only OA, provided that the parent toxins were within the range of detection and was not in an ester form.A good correlation was observed between the two methods when appropriate dilutions were performed.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire de Biochimie, Faculté des Sciences, Université Chouaib Doukkali El Jadida, Marocco.

ABSTRACT
Diarrheic shellfish poisoning (DSP) is a recurrent gastrointestinal illness in Morocco, resulting from consumption of contaminated shellfish. In order to develop a rapid and reliable technique for toxins detection, we have compared the results obtained by a commercial immunoassay-"DSP-Check" kit" with those obtained by LC-MS. Both techniques are capable of detecting the toxins in the whole flesh extract which was subjected to prior alkaline hydrolysis in order to detect simultaneously the esterified and non esterified toxin forms. The LC-MS method was found to be able to detect a high level of okadaic acid (OA), low level of dinophysistoxin-2 (DTX2), and surprisingly, traces of azaspiracids 2 (AZA2) in mussels. This is the first report of a survey carried out for azaspiracid (AZP) contamination of shellfish harvested in the coastal areas of Morocco. The "DSP-Check" kit was found to detect quantitatively DSP toxins in all contaminated samples containing only OA, provided that the parent toxins were within the range of detection and was not in an ester form. A good correlation was observed between the two methods when appropriate dilutions were performed. The immunoassay kit appeared to be more sensitive, specific and faster than LC-MS for determination of DSP in total shellfish extract.

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Related in: MedlinePlus

Evolution of OA and DTX2 (μg/100g of edible tissues) in mussels from Oualidia lagoon harvested between May and August 2006 (a) and their respective percentages (b).
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f1-md-06-00587: Evolution of OA and DTX2 (μg/100g of edible tissues) in mussels from Oualidia lagoon harvested between May and August 2006 (a) and their respective percentages (b).

Mentions: Figure 1a shows the contamination of DSP in the mussels with both OA and DTX2. OA was present in high concentrations, 19 to 135 μg/100g, from May to August 2006, exceeding the public health safety threshold of 16 μg/100 g of edible tissues. The contamination depended on the period of collection and the highest level of DSP was registered in June, while the lowest level was found in May. During the toxic season, the percentage of DTX2 was from 9 to 23 % of total DSP toxins (Figure 1b). However, the level registered did not exceed the safety threshold.


Detection of diarrheic shellfish poisoning and azaspiracid toxins in Moroccan mussels: comparison of the LC-MS method with the commercial immunoassay kit.

Elgarch A, Vale P, Rifai S, Fassouane A - Mar Drugs (2008)

Evolution of OA and DTX2 (μg/100g of edible tissues) in mussels from Oualidia lagoon harvested between May and August 2006 (a) and their respective percentages (b).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2630846&req=5

f1-md-06-00587: Evolution of OA and DTX2 (μg/100g of edible tissues) in mussels from Oualidia lagoon harvested between May and August 2006 (a) and their respective percentages (b).
Mentions: Figure 1a shows the contamination of DSP in the mussels with both OA and DTX2. OA was present in high concentrations, 19 to 135 μg/100g, from May to August 2006, exceeding the public health safety threshold of 16 μg/100 g of edible tissues. The contamination depended on the period of collection and the highest level of DSP was registered in June, while the lowest level was found in May. During the toxic season, the percentage of DTX2 was from 9 to 23 % of total DSP toxins (Figure 1b). However, the level registered did not exceed the safety threshold.

Bottom Line: The LC-MS method was found to be able to detect a high level of okadaic acid (OA), low level of dinophysistoxin-2 (DTX2), and surprisingly, traces of azaspiracids 2 (AZA2) in mussels.The "DSP-Check" kit was found to detect quantitatively DSP toxins in all contaminated samples containing only OA, provided that the parent toxins were within the range of detection and was not in an ester form.A good correlation was observed between the two methods when appropriate dilutions were performed.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire de Biochimie, Faculté des Sciences, Université Chouaib Doukkali El Jadida, Marocco.

ABSTRACT
Diarrheic shellfish poisoning (DSP) is a recurrent gastrointestinal illness in Morocco, resulting from consumption of contaminated shellfish. In order to develop a rapid and reliable technique for toxins detection, we have compared the results obtained by a commercial immunoassay-"DSP-Check" kit" with those obtained by LC-MS. Both techniques are capable of detecting the toxins in the whole flesh extract which was subjected to prior alkaline hydrolysis in order to detect simultaneously the esterified and non esterified toxin forms. The LC-MS method was found to be able to detect a high level of okadaic acid (OA), low level of dinophysistoxin-2 (DTX2), and surprisingly, traces of azaspiracids 2 (AZA2) in mussels. This is the first report of a survey carried out for azaspiracid (AZP) contamination of shellfish harvested in the coastal areas of Morocco. The "DSP-Check" kit was found to detect quantitatively DSP toxins in all contaminated samples containing only OA, provided that the parent toxins were within the range of detection and was not in an ester form. A good correlation was observed between the two methods when appropriate dilutions were performed. The immunoassay kit appeared to be more sensitive, specific and faster than LC-MS for determination of DSP in total shellfish extract.

Show MeSH
Related in: MedlinePlus