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Possible new hepatitis B virus genotype, southeast Asia.

Olinger CM, Jutavijittum P, Hübschen JM, Yousukh A, Samountry B, Thammavong T, Toriyama K, Muller CP - Emerging Infect. Dis. (2008)

Bottom Line: We conducted a phylogenetic analysis of 19 hepatitis B virus strains from Laos that belonged to 2 subgenotypes of a new genotype I.This emerging new genotype likely developed outside Southeast Asia and is now found in mixed infections and in recombinations with local strains in a geographically confined region.

View Article: PubMed Central - PubMed

Affiliation: National Public Health Laboratory/Centre de Recherche Public-Santé, Luxembourg, Luxembourg.

ABSTRACT
We conducted a phylogenetic analysis of 19 hepatitis B virus strains from Laos that belonged to 2 subgenotypes of a new genotype I. This emerging new genotype likely developed outside Southeast Asia and is now found in mixed infections and in recombinations with local strains in a geographically confined region.

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Related in: MedlinePlus

Bootscan analysis of genotypes I, A, B, C, and G compared with genotypes A–H. Data points correspond to the center of sequence windows of 800 bp. For the analysis of the first 400 nt, the beginning of the genome was duplicated at the end of the sequence: nt 3200–4000 represent positions 0–800.
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Figure 2: Bootscan analysis of genotypes I, A, B, C, and G compared with genotypes A–H. Data points correspond to the center of sequence windows of 800 bp. For the analysis of the first 400 nt, the beginning of the genome was duplicated at the end of the sequence: nt 3200–4000 represent positions 0–800.

Mentions: Most current genotypes of HBV seem to be the result of 1 or several recombination events (5). In particular, this is evident for the B/C recombinant, which has spread in mainland Asia (6) and has been defined as genotype Ba. Also genotypes B and C show some similarity with each other (Figure 2). Bootscan analysis (7) of all genotype I strains, including M04–3665, amplified by complete genome PCR, showed similarities with genotype C (nt 1400–3000), A (nt 3000–400), and G (nt 400–1400) by using a window size of 800 nt (Figure 2). Smaller bootscan windows tended to blur the relatedness. Phylogenetic reconstruction (Figure 1, panels B–D) and BLAST searches (http://blast.ncbi.nlm.nih.gov) (Table 2) of the above fragments of genotype I sequences confirmed the results of the bootscan analysis, which suggests that this genotype may also have evolved from a series of recombination events in a distant past.


Possible new hepatitis B virus genotype, southeast Asia.

Olinger CM, Jutavijittum P, Hübschen JM, Yousukh A, Samountry B, Thammavong T, Toriyama K, Muller CP - Emerging Infect. Dis. (2008)

Bootscan analysis of genotypes I, A, B, C, and G compared with genotypes A–H. Data points correspond to the center of sequence windows of 800 bp. For the analysis of the first 400 nt, the beginning of the genome was duplicated at the end of the sequence: nt 3200–4000 represent positions 0–800.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2630741&req=5

Figure 2: Bootscan analysis of genotypes I, A, B, C, and G compared with genotypes A–H. Data points correspond to the center of sequence windows of 800 bp. For the analysis of the first 400 nt, the beginning of the genome was duplicated at the end of the sequence: nt 3200–4000 represent positions 0–800.
Mentions: Most current genotypes of HBV seem to be the result of 1 or several recombination events (5). In particular, this is evident for the B/C recombinant, which has spread in mainland Asia (6) and has been defined as genotype Ba. Also genotypes B and C show some similarity with each other (Figure 2). Bootscan analysis (7) of all genotype I strains, including M04–3665, amplified by complete genome PCR, showed similarities with genotype C (nt 1400–3000), A (nt 3000–400), and G (nt 400–1400) by using a window size of 800 nt (Figure 2). Smaller bootscan windows tended to blur the relatedness. Phylogenetic reconstruction (Figure 1, panels B–D) and BLAST searches (http://blast.ncbi.nlm.nih.gov) (Table 2) of the above fragments of genotype I sequences confirmed the results of the bootscan analysis, which suggests that this genotype may also have evolved from a series of recombination events in a distant past.

Bottom Line: We conducted a phylogenetic analysis of 19 hepatitis B virus strains from Laos that belonged to 2 subgenotypes of a new genotype I.This emerging new genotype likely developed outside Southeast Asia and is now found in mixed infections and in recombinations with local strains in a geographically confined region.

View Article: PubMed Central - PubMed

Affiliation: National Public Health Laboratory/Centre de Recherche Public-Santé, Luxembourg, Luxembourg.

ABSTRACT
We conducted a phylogenetic analysis of 19 hepatitis B virus strains from Laos that belonged to 2 subgenotypes of a new genotype I. This emerging new genotype likely developed outside Southeast Asia and is now found in mixed infections and in recombinations with local strains in a geographically confined region.

Show MeSH
Related in: MedlinePlus