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Unusual Cryptosporidium genotypes in human cases of diarrhea.

Robinson G, Elwin K, Chalmers RM - Emerging Infect. Dis. (2008)

Bottom Line: Several Cryptosporidium spp. are known to infect humans, but most cases of illness are caused by Cryptosporidium hominis or C. parvum.During a long-term genotyping in the United Kingdom, we identified 3 unusual Cryptosporidium genotypes (skunk, horse, and rabbit) in human patients with diarrhea.

View Article: PubMed Central - PubMed

Affiliation: UK Cryptosporidium Reference Unit, Swansea, UK.

ABSTRACT
Several Cryptosporidium spp. are known to infect humans, but most cases of illness are caused by Cryptosporidium hominis or C. parvum. During a long-term genotyping in the United Kingdom, we identified 3 unusual Cryptosporidium genotypes (skunk, horse, and rabbit) in human patients with diarrhea.

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Phylogenetic relationships between 3 unusual Cryptosporidium genotypes and known Cryptosporidium species/genotypes as inferred by a neighbor-joining analysis of the small subunit rRNA gene. Evolutionary distances were calculated by the Kimura 2-parameter model with Eimeria tenella as an outgroup. Bootstrapping values >50% from 1,000 pseudoreplicates are shown at branches.
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Figure 1: Phylogenetic relationships between 3 unusual Cryptosporidium genotypes and known Cryptosporidium species/genotypes as inferred by a neighbor-joining analysis of the small subunit rRNA gene. Evolutionary distances were calculated by the Kimura 2-parameter model with Eimeria tenella as an outgroup. Bootstrapping values >50% from 1,000 pseudoreplicates are shown at branches.

Mentions: At the SSU rRNA and HSP70 genes, sequence analysis confirmed that W971, W6863, and W16103 were skunk, horse, and rabbit genotypes, respectively (Table). Isolate W971 was homologous with genotype W13 found in storm water, which, in turn, is the skunk genotype (6). Initially, the BLAST search for isolate W6863 erroneously indicated C. parvum as the most probable identity at the SSU rRNA gene, but this was due to the short length (484 bp) of the only horse genotype sequence available (AY273770) for comparison. Thus, C. parvum isolates that spanned our whole query sequence (787 bp) were calculated to have greater identities by BLAST. However, a detailed comparison between AY273770 and W6863 showed only 2-bp differences (including 1 insertion in our sequence) compared with 7-bp differences between W6863 and C. parvum. W6863 was confirmed as a variant of the horse genotype by HSP70 gene sequence analysis and SSU rRNA gene phylogenetic analysis (Figure).


Unusual Cryptosporidium genotypes in human cases of diarrhea.

Robinson G, Elwin K, Chalmers RM - Emerging Infect. Dis. (2008)

Phylogenetic relationships between 3 unusual Cryptosporidium genotypes and known Cryptosporidium species/genotypes as inferred by a neighbor-joining analysis of the small subunit rRNA gene. Evolutionary distances were calculated by the Kimura 2-parameter model with Eimeria tenella as an outgroup. Bootstrapping values >50% from 1,000 pseudoreplicates are shown at branches.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2630733&req=5

Figure 1: Phylogenetic relationships between 3 unusual Cryptosporidium genotypes and known Cryptosporidium species/genotypes as inferred by a neighbor-joining analysis of the small subunit rRNA gene. Evolutionary distances were calculated by the Kimura 2-parameter model with Eimeria tenella as an outgroup. Bootstrapping values >50% from 1,000 pseudoreplicates are shown at branches.
Mentions: At the SSU rRNA and HSP70 genes, sequence analysis confirmed that W971, W6863, and W16103 were skunk, horse, and rabbit genotypes, respectively (Table). Isolate W971 was homologous with genotype W13 found in storm water, which, in turn, is the skunk genotype (6). Initially, the BLAST search for isolate W6863 erroneously indicated C. parvum as the most probable identity at the SSU rRNA gene, but this was due to the short length (484 bp) of the only horse genotype sequence available (AY273770) for comparison. Thus, C. parvum isolates that spanned our whole query sequence (787 bp) were calculated to have greater identities by BLAST. However, a detailed comparison between AY273770 and W6863 showed only 2-bp differences (including 1 insertion in our sequence) compared with 7-bp differences between W6863 and C. parvum. W6863 was confirmed as a variant of the horse genotype by HSP70 gene sequence analysis and SSU rRNA gene phylogenetic analysis (Figure).

Bottom Line: Several Cryptosporidium spp. are known to infect humans, but most cases of illness are caused by Cryptosporidium hominis or C. parvum.During a long-term genotyping in the United Kingdom, we identified 3 unusual Cryptosporidium genotypes (skunk, horse, and rabbit) in human patients with diarrhea.

View Article: PubMed Central - PubMed

Affiliation: UK Cryptosporidium Reference Unit, Swansea, UK.

ABSTRACT
Several Cryptosporidium spp. are known to infect humans, but most cases of illness are caused by Cryptosporidium hominis or C. parvum. During a long-term genotyping in the United Kingdom, we identified 3 unusual Cryptosporidium genotypes (skunk, horse, and rabbit) in human patients with diarrhea.

Show MeSH
Related in: MedlinePlus