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Bacteremia caused by Mycobacterium wolinskyi.

Chen YC, Jou R, Huang WL, Huang ST, Liu KC, Lay CJ, Chang SM, Tseng CE, Lai CL, Su YC - Emerging Infect. Dis. (2008)

View Article: PubMed Central - PubMed

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Mycobacterium wolinskyi is a rapidly growing mycobacterium that belongs to the M. smegmatis group, which includes M. smegmatis sensu stricto and 2 species described in 1999 (M. goodii and M. wolinskyi)... We drew 2 sets of blood samples from a peripheral vein for culture and incubated these cultures (BACTEC 9240 Continuous Monitoring Blood Culture System; Becton Dickinson, Sparks, MD, USA) using BACTEC Aerobic Plus and Anaerobic Plus medium (Becton Dickinson)... For sequencing 16S rDNA, we used either the primer 16S-27f or 16S-519r (5′-GWA TTA CCG CGG CKG CTG-3′)... We performed both forward and reverse (5′ and 3′) sequencing... For accurate analysis of the data, a 492-bp variable region (corresponding to positions 27– 519) was carefully analyzed after it was compared with sequences of Mycobacterium spp. in the BLAST database (www.ncbi.nlm.nih.gov), as described... The results showed 99% similarity between our isolate and M. wolinskyi... A few days later, we obtained synovial fluid by needle biopsy and cultured samples in BACTEC Aerobic Plus and Anaerobic Plus medium (Becton Dickinson) and on trypticase soy agar... Surgical debridement followed by antimicrobial therapy for at least 6 months is the suggested treatment for M. wolinskyi infection, and we followed this regimen... Because of the frequency of relapse and resistance, we used combination therapy with multiple antimicrobial agents... This case suggests that immunocompromised patients may be vulnerable to infection by rapidly growing mycobacterium such as M. wolinskyi... In such cases, we suggest antimicrobial drug treatment, based on in vitro susceptibility.

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Histologic image of debrided tissue of the patient, showing inflammatory processes within the synovial tissue and the presence of an acid-fast bacillus (magnification ×400, acid-fast stain).
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Figure 1: Histologic image of debrided tissue of the patient, showing inflammatory processes within the synovial tissue and the presence of an acid-fast bacillus (magnification ×400, acid-fast stain).

Mentions: A few days later, we obtained synovial fluid by needle biopsy and cultured samples in BACTEC Aerobic Plus and Anaerobic Plus medium (Becton Dickinson) and on trypticase soy agar. Within 3 days, these cultures were also positive for M. wolinskyi. Arthroscopically assisted arthrocentesis and debridement showed a turbid joint and the debrided tissue showed inflammatory processes within the synovial tissue and the presence of acid-fast bacilli (Figure). We grew cultures of acid-fast bacilli on trypticase soy agar after 2 to 4 days. The colonies were nonchromogenic, smooth to mucoid, and off-white to cream on Middlebrook 7H10 and trypticase soy agar.


Bacteremia caused by Mycobacterium wolinskyi.

Chen YC, Jou R, Huang WL, Huang ST, Liu KC, Lay CJ, Chang SM, Tseng CE, Lai CL, Su YC - Emerging Infect. Dis. (2008)

Histologic image of debrided tissue of the patient, showing inflammatory processes within the synovial tissue and the presence of an acid-fast bacillus (magnification ×400, acid-fast stain).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2630732&req=5

Figure 1: Histologic image of debrided tissue of the patient, showing inflammatory processes within the synovial tissue and the presence of an acid-fast bacillus (magnification ×400, acid-fast stain).
Mentions: A few days later, we obtained synovial fluid by needle biopsy and cultured samples in BACTEC Aerobic Plus and Anaerobic Plus medium (Becton Dickinson) and on trypticase soy agar. Within 3 days, these cultures were also positive for M. wolinskyi. Arthroscopically assisted arthrocentesis and debridement showed a turbid joint and the debrided tissue showed inflammatory processes within the synovial tissue and the presence of acid-fast bacilli (Figure). We grew cultures of acid-fast bacilli on trypticase soy agar after 2 to 4 days. The colonies were nonchromogenic, smooth to mucoid, and off-white to cream on Middlebrook 7H10 and trypticase soy agar.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Mycobacterium wolinskyi is a rapidly growing mycobacterium that belongs to the M. smegmatis group, which includes M. smegmatis sensu stricto and 2 species described in 1999 (M. goodii and M. wolinskyi)... We drew 2 sets of blood samples from a peripheral vein for culture and incubated these cultures (BACTEC 9240 Continuous Monitoring Blood Culture System; Becton Dickinson, Sparks, MD, USA) using BACTEC Aerobic Plus and Anaerobic Plus medium (Becton Dickinson)... For sequencing 16S rDNA, we used either the primer 16S-27f or 16S-519r (5′-GWA TTA CCG CGG CKG CTG-3′)... We performed both forward and reverse (5′ and 3′) sequencing... For accurate analysis of the data, a 492-bp variable region (corresponding to positions 27– 519) was carefully analyzed after it was compared with sequences of Mycobacterium spp. in the BLAST database (www.ncbi.nlm.nih.gov), as described... The results showed 99% similarity between our isolate and M. wolinskyi... A few days later, we obtained synovial fluid by needle biopsy and cultured samples in BACTEC Aerobic Plus and Anaerobic Plus medium (Becton Dickinson) and on trypticase soy agar... Surgical debridement followed by antimicrobial therapy for at least 6 months is the suggested treatment for M. wolinskyi infection, and we followed this regimen... Because of the frequency of relapse and resistance, we used combination therapy with multiple antimicrobial agents... This case suggests that immunocompromised patients may be vulnerable to infection by rapidly growing mycobacterium such as M. wolinskyi... In such cases, we suggest antimicrobial drug treatment, based on in vitro susceptibility.

Show MeSH