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Chronic brucellosis patients retain low frequency of CD4+ T-lymphocytes expressing CD25 and CD28 after Escherichia coli LPS stimulation of PHA-cultured PBMCs.

Skendros P, Sarantopoulos A, Tselios K, Boura P - Clin. Dev. Immunol. (2009)

Bottom Line: A significant decrease in the percentage of CD4+/CD25+ and CD4+/CD28+ T-lymphocytes was observed in CB compared to AB.In HKBA cultures, compared to E. coli LPS-cultures, there was a significant reduction of CD4+/CD25+ T-lymphocytes in all groups and CD14+/CD80+ in patients groups.We suggest that Brucella can modulate host immune response, leading to T-cell anergy and chronic infection.

View Article: PubMed Central - PubMed

Affiliation: Clinical Immunology Unit, 2nd Department of Internal Medicine, Hippokration General Hospital, Aristotle University of Thessaloniki, Konstantinoupoleos Street 49, 546 42 Thessaloniki, Greece.

ABSTRACT
Chronic brucellosis patients display a defective Th1 response to PHA. We have previously shown that heat-killed B. abortus (HKBA) can downregulate the PHA-induced increase of CD4+/CD25+ and CD14+/CD80+ cells of brucellosis patients. In the present study, we investigate the effect of E. coli LPS, as a potent stimulant of monocytes and autologous T-lymphocytes, on the PHA-cultured PBMCs of the same groups of patients. Thirteen acute brucellosis (AB) patients, 22 chronic brucellosis (CB) patients, 11 "cured" subjects, and 15 healthy volunteers were studied. The percentage of CD4+/CD25+ and CD4+/CD28+ T-lymphocytes as well as CD14+/CD80+ monocytes were analyzed by flow cytometry after PBMCs culture with PHA plus E. coli LPS. A significant decrease in the percentage of CD4+/CD25+ and CD4+/CD28+ T-lymphocytes was observed in CB compared to AB. In HKBA cultures, compared to E. coli LPS-cultures, there was a significant reduction of CD4+/CD25+ T-lymphocytes in all groups and CD14+/CD80+ in patients groups. We suggest that Brucella can modulate host immune response, leading to T-cell anergy and chronic infection.

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Related in: MedlinePlus

Comparison of the PHA-cultures. HKBA additiondownregulates (a)  the percentages of CD4+/CD25+T-cells in all groups and (b) CD14+/CD80+ monocytes in brucellosis patients. Data represent mean values+SD (SE). #P  ≤  .05;PHA versus PHA  +  E. coli LPS, ⋀P  ≤  .05; ⋀⋀P  <  .001; PHA versus PHA  +  HKBA,  *P  ≤  .05; **P  <  .001; PHA  +  E. coli LPS versus PHA  +  HKBA.
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fig1: Comparison of the PHA-cultures. HKBA additiondownregulates (a) the percentages of CD4+/CD25+T-cells in all groups and (b) CD14+/CD80+ monocytes in brucellosis patients. Data represent mean values+SD (SE). #P ≤ .05;PHA versus PHA + E. coli LPS, ⋀P ≤ .05; ⋀⋀P < .001; PHA versus PHA + HKBA, *P ≤ .05; **P < .001; PHA + E. coli LPS versus PHA + HKBA.

Mentions: Wecompared the above results with the findings yielded from our previous studies(on the same cultured PBMCs stimulated simultaneously with PHA alone or PHAplus HKBA, Figure 1) [21, 23]. The results between PHA alone and PHA plus E.coli LPS-cultured PBMCs were similar. On the contrary, when HKBA-cultures,compared to E. coli LPS-cultures, there was a significant reduction of CD4+/CD25+ T-lymphocytes inall groups (AB P = .005, CB P = .042, “cured” P = .029, controls P = .007) and CD14+/CD80+ monocytes in patients groups (AB P < .001,CB P = .05) (Figure 1).


Chronic brucellosis patients retain low frequency of CD4+ T-lymphocytes expressing CD25 and CD28 after Escherichia coli LPS stimulation of PHA-cultured PBMCs.

Skendros P, Sarantopoulos A, Tselios K, Boura P - Clin. Dev. Immunol. (2009)

Comparison of the PHA-cultures. HKBA additiondownregulates (a)  the percentages of CD4+/CD25+T-cells in all groups and (b) CD14+/CD80+ monocytes in brucellosis patients. Data represent mean values+SD (SE). #P  ≤  .05;PHA versus PHA  +  E. coli LPS, ⋀P  ≤  .05; ⋀⋀P  <  .001; PHA versus PHA  +  HKBA,  *P  ≤  .05; **P  <  .001; PHA  +  E. coli LPS versus PHA  +  HKBA.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2630417&req=5

fig1: Comparison of the PHA-cultures. HKBA additiondownregulates (a) the percentages of CD4+/CD25+T-cells in all groups and (b) CD14+/CD80+ monocytes in brucellosis patients. Data represent mean values+SD (SE). #P ≤ .05;PHA versus PHA + E. coli LPS, ⋀P ≤ .05; ⋀⋀P < .001; PHA versus PHA + HKBA, *P ≤ .05; **P < .001; PHA + E. coli LPS versus PHA + HKBA.
Mentions: Wecompared the above results with the findings yielded from our previous studies(on the same cultured PBMCs stimulated simultaneously with PHA alone or PHAplus HKBA, Figure 1) [21, 23]. The results between PHA alone and PHA plus E.coli LPS-cultured PBMCs were similar. On the contrary, when HKBA-cultures,compared to E. coli LPS-cultures, there was a significant reduction of CD4+/CD25+ T-lymphocytes inall groups (AB P = .005, CB P = .042, “cured” P = .029, controls P = .007) and CD14+/CD80+ monocytes in patients groups (AB P < .001,CB P = .05) (Figure 1).

Bottom Line: A significant decrease in the percentage of CD4+/CD25+ and CD4+/CD28+ T-lymphocytes was observed in CB compared to AB.In HKBA cultures, compared to E. coli LPS-cultures, there was a significant reduction of CD4+/CD25+ T-lymphocytes in all groups and CD14+/CD80+ in patients groups.We suggest that Brucella can modulate host immune response, leading to T-cell anergy and chronic infection.

View Article: PubMed Central - PubMed

Affiliation: Clinical Immunology Unit, 2nd Department of Internal Medicine, Hippokration General Hospital, Aristotle University of Thessaloniki, Konstantinoupoleos Street 49, 546 42 Thessaloniki, Greece.

ABSTRACT
Chronic brucellosis patients display a defective Th1 response to PHA. We have previously shown that heat-killed B. abortus (HKBA) can downregulate the PHA-induced increase of CD4+/CD25+ and CD14+/CD80+ cells of brucellosis patients. In the present study, we investigate the effect of E. coli LPS, as a potent stimulant of monocytes and autologous T-lymphocytes, on the PHA-cultured PBMCs of the same groups of patients. Thirteen acute brucellosis (AB) patients, 22 chronic brucellosis (CB) patients, 11 "cured" subjects, and 15 healthy volunteers were studied. The percentage of CD4+/CD25+ and CD4+/CD28+ T-lymphocytes as well as CD14+/CD80+ monocytes were analyzed by flow cytometry after PBMCs culture with PHA plus E. coli LPS. A significant decrease in the percentage of CD4+/CD25+ and CD4+/CD28+ T-lymphocytes was observed in CB compared to AB. In HKBA cultures, compared to E. coli LPS-cultures, there was a significant reduction of CD4+/CD25+ T-lymphocytes in all groups and CD14+/CD80+ in patients groups. We suggest that Brucella can modulate host immune response, leading to T-cell anergy and chronic infection.

Show MeSH
Related in: MedlinePlus