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A comprehensive characterisation of the fibre composition and properties of a limb (flexor digitorum superficialis, membri thoraci) and a trunk (psoas major) muscle in cattle.

Moreno-Sánchez N, Díaz C, Carabaño MJ, Rueda J, Rivero JL - BMC Cell Biol. (2008)

Bottom Line: All fibre types were found in PM, the IIX type being the most frequent.Correlations among contractile, metabolic and histological features on individual fibres were significantly different from zero (r values varied between -0.31 and 0.78).These results support the concept that, to some extent, muscle plasticity can be explained by the fibre type composition, and by the properties derived from their metabolic and histological profiles.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.

ABSTRACT

Background: The fibre type attributes and the relationships among their properties play an important role in the differences in muscle capabilities and features. Comprehensive characterisation of the skeletal muscles should study the degree of association between them and their involvement in muscle functionality. The purposes of the present study were to characterise the fibre type composition of a trunk (Psoas major, PM) and a limb (Flexor digitorum, membri thoraci, FD) muscle in the bovine species and to study the degree of coordination among contractile, metabolic and histological properties of fibre types. Immunohistochemical, histochemical and histological techniques were used.

Results: The fibre type composition was delineated immunohistochemically in calf muscle samples, identifying three pure (I, IIA, and IIX) and two hybrid type fibres (I+IIA, and IIAX). Most of the fibres in FD were types I and IIA, while pure IIX were absent. All fibre types were found in PM, the IIX type being the most frequent. Compared to other species, small populations of hybrid fibres were detected. The five fibre types, previously identified, were ascribed to three different acid and alkaline mATPase activity patterns. Type I fibres had the highest oxidative capacity and the lowest glycolytic capacity. The reverse was true for the IIX fibres, whereas the type IIA fibres showed intermediate properties. Regarding the histological properties, type I fibres tended to be more capillarised than the II types. Correlations among contractile, metabolic and histological features on individual fibres were significantly different from zero (r values varied between -0.31 and 0.78). Hybrid fibre values were positioned between their corresponding pure types, and their positions were different regarding their metabolic and contractile properties.

Conclusion: Coordination among the contractile, metabolic and histological properties of fibres has been observed. However, the magnitude of the correlation among them is always below 0.8, suggesting that the properties of muscles are not fully explained by the fibre composition. These results support the concept that, to some extent, muscle plasticity can be explained by the fibre type composition, and by the properties derived from their metabolic and histological profiles.

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Normalized mean OD of SDH and GPDH activities and their relationships to the fibre types. A, B: OD standardised to Z scores of SDH and GPDH activities of the MyHC-based fibre types. Mean OD for SDH and GPDH (A). SDH:GPDH ratio (B). Values are pooled means of the 814 fibres analysed in the two skeletal muscles (PM and FD). Vertical bars represent the 0.95 confidence intervals. C, D: Fibre-to-fibre relationships between OD of the immunostaining and SDH and GPDH histochemical activities in various subsets of hybrid fibres. Relationship between the S58H2 MAb (anti MyHC I and IIX) and both SDH and GPDH staining of all I+IIA hybrid fibres identified in the present study (n = 42 fibres) (C). Relationship between the BF35 MAb (anti MyHC I and IIA) and both SDH and GPDH staining of all IIAX hybrid fibres identified in the study (n = 59 fibres) (D). r: Pearson coefficient of correlation; #, *: p < 0.001 and p < 0.05 levels of significance, respectively.
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Figure 4: Normalized mean OD of SDH and GPDH activities and their relationships to the fibre types. A, B: OD standardised to Z scores of SDH and GPDH activities of the MyHC-based fibre types. Mean OD for SDH and GPDH (A). SDH:GPDH ratio (B). Values are pooled means of the 814 fibres analysed in the two skeletal muscles (PM and FD). Vertical bars represent the 0.95 confidence intervals. C, D: Fibre-to-fibre relationships between OD of the immunostaining and SDH and GPDH histochemical activities in various subsets of hybrid fibres. Relationship between the S58H2 MAb (anti MyHC I and IIX) and both SDH and GPDH staining of all I+IIA hybrid fibres identified in the present study (n = 42 fibres) (C). Relationship between the BF35 MAb (anti MyHC I and IIA) and both SDH and GPDH staining of all IIAX hybrid fibres identified in the study (n = 59 fibres) (D). r: Pearson coefficient of correlation; #, *: p < 0.001 and p < 0.05 levels of significance, respectively.

Mentions: The visual examination of SDH and GPDH histochemical reactions revealed a continuous variation in the staining intensities of all fibre types (Figure 1G–1H). Significant differences were detected among fibre types for SDH and GPDH histochemical activities (Figure 4). On average, SDH mean activities tended to decrease significantly from type I to type IIX fibres, whereas GPDH mean activities showed the reverse tendency (Figure 4A). Hybrid I+IIA fibres were slightly closer to IIA than to I, and IIAX were closer to IIA than to IIX (Figure 4A). Therefore, the ratio SDH:GPDH, which is commonly used as an indicator of the rate between oxidative and glycolytic metabolisms of myofibres, decreased consistently, as shown in Figure 4B. SDH activity was higher than GPDH activity in all fibre types but IIX, which showed higher GPDH than SDH activities. A negative correlation (r = -0.49) was found between SDH and GPDH activities.


A comprehensive characterisation of the fibre composition and properties of a limb (flexor digitorum superficialis, membri thoraci) and a trunk (psoas major) muscle in cattle.

Moreno-Sánchez N, Díaz C, Carabaño MJ, Rueda J, Rivero JL - BMC Cell Biol. (2008)

Normalized mean OD of SDH and GPDH activities and their relationships to the fibre types. A, B: OD standardised to Z scores of SDH and GPDH activities of the MyHC-based fibre types. Mean OD for SDH and GPDH (A). SDH:GPDH ratio (B). Values are pooled means of the 814 fibres analysed in the two skeletal muscles (PM and FD). Vertical bars represent the 0.95 confidence intervals. C, D: Fibre-to-fibre relationships between OD of the immunostaining and SDH and GPDH histochemical activities in various subsets of hybrid fibres. Relationship between the S58H2 MAb (anti MyHC I and IIX) and both SDH and GPDH staining of all I+IIA hybrid fibres identified in the present study (n = 42 fibres) (C). Relationship between the BF35 MAb (anti MyHC I and IIA) and both SDH and GPDH staining of all IIAX hybrid fibres identified in the study (n = 59 fibres) (D). r: Pearson coefficient of correlation; #, *: p < 0.001 and p < 0.05 levels of significance, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2630315&req=5

Figure 4: Normalized mean OD of SDH and GPDH activities and their relationships to the fibre types. A, B: OD standardised to Z scores of SDH and GPDH activities of the MyHC-based fibre types. Mean OD for SDH and GPDH (A). SDH:GPDH ratio (B). Values are pooled means of the 814 fibres analysed in the two skeletal muscles (PM and FD). Vertical bars represent the 0.95 confidence intervals. C, D: Fibre-to-fibre relationships between OD of the immunostaining and SDH and GPDH histochemical activities in various subsets of hybrid fibres. Relationship between the S58H2 MAb (anti MyHC I and IIX) and both SDH and GPDH staining of all I+IIA hybrid fibres identified in the present study (n = 42 fibres) (C). Relationship between the BF35 MAb (anti MyHC I and IIA) and both SDH and GPDH staining of all IIAX hybrid fibres identified in the study (n = 59 fibres) (D). r: Pearson coefficient of correlation; #, *: p < 0.001 and p < 0.05 levels of significance, respectively.
Mentions: The visual examination of SDH and GPDH histochemical reactions revealed a continuous variation in the staining intensities of all fibre types (Figure 1G–1H). Significant differences were detected among fibre types for SDH and GPDH histochemical activities (Figure 4). On average, SDH mean activities tended to decrease significantly from type I to type IIX fibres, whereas GPDH mean activities showed the reverse tendency (Figure 4A). Hybrid I+IIA fibres were slightly closer to IIA than to I, and IIAX were closer to IIA than to IIX (Figure 4A). Therefore, the ratio SDH:GPDH, which is commonly used as an indicator of the rate between oxidative and glycolytic metabolisms of myofibres, decreased consistently, as shown in Figure 4B. SDH activity was higher than GPDH activity in all fibre types but IIX, which showed higher GPDH than SDH activities. A negative correlation (r = -0.49) was found between SDH and GPDH activities.

Bottom Line: All fibre types were found in PM, the IIX type being the most frequent.Correlations among contractile, metabolic and histological features on individual fibres were significantly different from zero (r values varied between -0.31 and 0.78).These results support the concept that, to some extent, muscle plasticity can be explained by the fibre type composition, and by the properties derived from their metabolic and histological profiles.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.

ABSTRACT

Background: The fibre type attributes and the relationships among their properties play an important role in the differences in muscle capabilities and features. Comprehensive characterisation of the skeletal muscles should study the degree of association between them and their involvement in muscle functionality. The purposes of the present study were to characterise the fibre type composition of a trunk (Psoas major, PM) and a limb (Flexor digitorum, membri thoraci, FD) muscle in the bovine species and to study the degree of coordination among contractile, metabolic and histological properties of fibre types. Immunohistochemical, histochemical and histological techniques were used.

Results: The fibre type composition was delineated immunohistochemically in calf muscle samples, identifying three pure (I, IIA, and IIX) and two hybrid type fibres (I+IIA, and IIAX). Most of the fibres in FD were types I and IIA, while pure IIX were absent. All fibre types were found in PM, the IIX type being the most frequent. Compared to other species, small populations of hybrid fibres were detected. The five fibre types, previously identified, were ascribed to three different acid and alkaline mATPase activity patterns. Type I fibres had the highest oxidative capacity and the lowest glycolytic capacity. The reverse was true for the IIX fibres, whereas the type IIA fibres showed intermediate properties. Regarding the histological properties, type I fibres tended to be more capillarised than the II types. Correlations among contractile, metabolic and histological features on individual fibres were significantly different from zero (r values varied between -0.31 and 0.78). Hybrid fibre values were positioned between their corresponding pure types, and their positions were different regarding their metabolic and contractile properties.

Conclusion: Coordination among the contractile, metabolic and histological properties of fibres has been observed. However, the magnitude of the correlation among them is always below 0.8, suggesting that the properties of muscles are not fully explained by the fibre composition. These results support the concept that, to some extent, muscle plasticity can be explained by the fibre type composition, and by the properties derived from their metabolic and histological profiles.

Show MeSH