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A comprehensive characterisation of the fibre composition and properties of a limb (flexor digitorum superficialis, membri thoraci) and a trunk (psoas major) muscle in cattle.

Moreno-Sánchez N, Díaz C, Carabaño MJ, Rueda J, Rivero JL - BMC Cell Biol. (2008)

Bottom Line: All fibre types were found in PM, the IIX type being the most frequent.Correlations among contractile, metabolic and histological features on individual fibres were significantly different from zero (r values varied between -0.31 and 0.78).These results support the concept that, to some extent, muscle plasticity can be explained by the fibre type composition, and by the properties derived from their metabolic and histological profiles.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.

ABSTRACT

Background: The fibre type attributes and the relationships among their properties play an important role in the differences in muscle capabilities and features. Comprehensive characterisation of the skeletal muscles should study the degree of association between them and their involvement in muscle functionality. The purposes of the present study were to characterise the fibre type composition of a trunk (Psoas major, PM) and a limb (Flexor digitorum, membri thoraci, FD) muscle in the bovine species and to study the degree of coordination among contractile, metabolic and histological properties of fibre types. Immunohistochemical, histochemical and histological techniques were used.

Results: The fibre type composition was delineated immunohistochemically in calf muscle samples, identifying three pure (I, IIA, and IIX) and two hybrid type fibres (I+IIA, and IIAX). Most of the fibres in FD were types I and IIA, while pure IIX were absent. All fibre types were found in PM, the IIX type being the most frequent. Compared to other species, small populations of hybrid fibres were detected. The five fibre types, previously identified, were ascribed to three different acid and alkaline mATPase activity patterns. Type I fibres had the highest oxidative capacity and the lowest glycolytic capacity. The reverse was true for the IIX fibres, whereas the type IIA fibres showed intermediate properties. Regarding the histological properties, type I fibres tended to be more capillarised than the II types. Correlations among contractile, metabolic and histological features on individual fibres were significantly different from zero (r values varied between -0.31 and 0.78). Hybrid fibre values were positioned between their corresponding pure types, and their positions were different regarding their metabolic and contractile properties.

Conclusion: Coordination among the contractile, metabolic and histological properties of fibres has been observed. However, the magnitude of the correlation among them is always below 0.8, suggesting that the properties of muscles are not fully explained by the fibre composition. These results support the concept that, to some extent, muscle plasticity can be explained by the fibre type composition, and by the properties derived from their metabolic and histological profiles.

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Serial sections of the PM muscle stained for immunohistochemistry, enzyme histochemistry and histology. A-D: Sections were stained with a battery of MAbs against specific MyHC isoforms: BAF8 anti MyHC I (A), SC71 anti MyHC IIA and IIX (B), BF35 anti MyHC I and IIA (C), and S58H2 anti MyHC I and IIX (D). E-F: Sections assayed for mATPase activity after acid (pH 4.42, E) and alkaline (pH 10.35, F) preincubations. G-I: Sections assayed for SDH (G), GPDH (H), and PAS for selective staining of glycogen (I). J-K: Histological staining with PAS after digestion with α-amylase to reveal capillaries (J) and haematoxylin-eosin to show total nuclei (K).
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Figure 1: Serial sections of the PM muscle stained for immunohistochemistry, enzyme histochemistry and histology. A-D: Sections were stained with a battery of MAbs against specific MyHC isoforms: BAF8 anti MyHC I (A), SC71 anti MyHC IIA and IIX (B), BF35 anti MyHC I and IIA (C), and S58H2 anti MyHC I and IIX (D). E-F: Sections assayed for mATPase activity after acid (pH 4.42, E) and alkaline (pH 10.35, F) preincubations. G-I: Sections assayed for SDH (G), GPDH (H), and PAS for selective staining of glycogen (I). J-K: Histological staining with PAS after digestion with α-amylase to reveal capillaries (J) and haematoxylin-eosin to show total nuclei (K).

Mentions: The fibre types were identified by visual inspection of sections stained with the anti-MyHCs MAbs. Three of them were pure fibre types expressing a unique MyHC isoform, either I, IIA or IIX, and two others were hybrid types co-expressing two MyHC isoforms, I plus IIA (I+IIA), and IIA plus IIX (IIAX). Type I fibres reacted with all MAbs except SC71 (e.g. fibre 1 in Figure 1A–D). Type IIA reacted with MAbs SC71 and BF35 but not with the remaining MAbs (e.g. fibre 3 in Figure 1A–D). Type IIX fibres were negative for MAbs BAF8 and BF35, and positive for S58H2 and SC71 MAbs (e.g. fibre 5 in Figure 1A–D). Hybrid I+IIA fibres reacted with all four MAbs (e.g. fibre 2 in Figure 1A–D), while IIAX fibres were labelled with all MAbs, except BAF8 (e.g. fibre 4 in Figure 1A–D). The fibre immunostaining with the specific anti-MyHC MAbs showed a wide range of reactions, not just positive or negative. In the hybrid types, the staining showed a continuous variation, possibly due to the differential contents of the MyHCs they are co-expressing.


A comprehensive characterisation of the fibre composition and properties of a limb (flexor digitorum superficialis, membri thoraci) and a trunk (psoas major) muscle in cattle.

Moreno-Sánchez N, Díaz C, Carabaño MJ, Rueda J, Rivero JL - BMC Cell Biol. (2008)

Serial sections of the PM muscle stained for immunohistochemistry, enzyme histochemistry and histology. A-D: Sections were stained with a battery of MAbs against specific MyHC isoforms: BAF8 anti MyHC I (A), SC71 anti MyHC IIA and IIX (B), BF35 anti MyHC I and IIA (C), and S58H2 anti MyHC I and IIX (D). E-F: Sections assayed for mATPase activity after acid (pH 4.42, E) and alkaline (pH 10.35, F) preincubations. G-I: Sections assayed for SDH (G), GPDH (H), and PAS for selective staining of glycogen (I). J-K: Histological staining with PAS after digestion with α-amylase to reveal capillaries (J) and haematoxylin-eosin to show total nuclei (K).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2630315&req=5

Figure 1: Serial sections of the PM muscle stained for immunohistochemistry, enzyme histochemistry and histology. A-D: Sections were stained with a battery of MAbs against specific MyHC isoforms: BAF8 anti MyHC I (A), SC71 anti MyHC IIA and IIX (B), BF35 anti MyHC I and IIA (C), and S58H2 anti MyHC I and IIX (D). E-F: Sections assayed for mATPase activity after acid (pH 4.42, E) and alkaline (pH 10.35, F) preincubations. G-I: Sections assayed for SDH (G), GPDH (H), and PAS for selective staining of glycogen (I). J-K: Histological staining with PAS after digestion with α-amylase to reveal capillaries (J) and haematoxylin-eosin to show total nuclei (K).
Mentions: The fibre types were identified by visual inspection of sections stained with the anti-MyHCs MAbs. Three of them were pure fibre types expressing a unique MyHC isoform, either I, IIA or IIX, and two others were hybrid types co-expressing two MyHC isoforms, I plus IIA (I+IIA), and IIA plus IIX (IIAX). Type I fibres reacted with all MAbs except SC71 (e.g. fibre 1 in Figure 1A–D). Type IIA reacted with MAbs SC71 and BF35 but not with the remaining MAbs (e.g. fibre 3 in Figure 1A–D). Type IIX fibres were negative for MAbs BAF8 and BF35, and positive for S58H2 and SC71 MAbs (e.g. fibre 5 in Figure 1A–D). Hybrid I+IIA fibres reacted with all four MAbs (e.g. fibre 2 in Figure 1A–D), while IIAX fibres were labelled with all MAbs, except BAF8 (e.g. fibre 4 in Figure 1A–D). The fibre immunostaining with the specific anti-MyHC MAbs showed a wide range of reactions, not just positive or negative. In the hybrid types, the staining showed a continuous variation, possibly due to the differential contents of the MyHCs they are co-expressing.

Bottom Line: All fibre types were found in PM, the IIX type being the most frequent.Correlations among contractile, metabolic and histological features on individual fibres were significantly different from zero (r values varied between -0.31 and 0.78).These results support the concept that, to some extent, muscle plasticity can be explained by the fibre type composition, and by the properties derived from their metabolic and histological profiles.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.

ABSTRACT

Background: The fibre type attributes and the relationships among their properties play an important role in the differences in muscle capabilities and features. Comprehensive characterisation of the skeletal muscles should study the degree of association between them and their involvement in muscle functionality. The purposes of the present study were to characterise the fibre type composition of a trunk (Psoas major, PM) and a limb (Flexor digitorum, membri thoraci, FD) muscle in the bovine species and to study the degree of coordination among contractile, metabolic and histological properties of fibre types. Immunohistochemical, histochemical and histological techniques were used.

Results: The fibre type composition was delineated immunohistochemically in calf muscle samples, identifying three pure (I, IIA, and IIX) and two hybrid type fibres (I+IIA, and IIAX). Most of the fibres in FD were types I and IIA, while pure IIX were absent. All fibre types were found in PM, the IIX type being the most frequent. Compared to other species, small populations of hybrid fibres were detected. The five fibre types, previously identified, were ascribed to three different acid and alkaline mATPase activity patterns. Type I fibres had the highest oxidative capacity and the lowest glycolytic capacity. The reverse was true for the IIX fibres, whereas the type IIA fibres showed intermediate properties. Regarding the histological properties, type I fibres tended to be more capillarised than the II types. Correlations among contractile, metabolic and histological features on individual fibres were significantly different from zero (r values varied between -0.31 and 0.78). Hybrid fibre values were positioned between their corresponding pure types, and their positions were different regarding their metabolic and contractile properties.

Conclusion: Coordination among the contractile, metabolic and histological properties of fibres has been observed. However, the magnitude of the correlation among them is always below 0.8, suggesting that the properties of muscles are not fully explained by the fibre composition. These results support the concept that, to some extent, muscle plasticity can be explained by the fibre type composition, and by the properties derived from their metabolic and histological profiles.

Show MeSH
Related in: MedlinePlus