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Q fever in young children, Ghana.

Kobbe R, Kramme S, Kreuels B, Adjei S, Kreuzberg C, Panning M, Adjei O, Fleischer B, May J - Emerging Infect. Dis. (2008)

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To identify all children with Q fever titers, we regarded the following as positive fluorescence reactions to plasma dilutions: >1:64 for phase II immunoglobulin (Ig) G and >1:24 for phase II IgM with sensitivity (specificity) of 97.2% (100%) and 100% (56.3%), respectively... IgM testing was only performed on IgG-positive children... On the day of the serosurvey 71 (32.4%) of 219 children had fever (measured body temperature >38°C or reported fever within the previous 48 hours)... Test outcome did not appear to be influenced by P. falciparum infection, since 4 of 37 IgG-positive children (23 of 182 IgG-negative children) had clinical malaria, 11/37 (62/182) had asymptomatic parasitemia, and 6/37 (38/182) had fever without parasitemia, and there were no significant differences between groups... The frequency of prior malaria episodes also did not influence antibody response... Three aparasitemic children had positive phase II IgM titers (24, 96, and 1,536; phase II IgG 64, 64, and 4,096, respectively)... The child with the high IgM and IgG titers was clinically ill with nonsevere C. burnetii pneumonia... This child was among 10 (27%) of 37 phase II IgG-positive children with detectable anti–C. burnetii phase I antibodies... Children, especially those of illiterate mothers, could also be more frequently exposed to the pathogen... Consumption of unpasteurized dairy products can result in infection or seroconversion without clinical disease... However, because consumption of raw milk in the Ashanti region is regarded as being uncommon by local health authorities, we consider dairy products an unlikely source of the disease... Although participants were intensively exposed to P. falciparum, which causes polyclonal B-cell stimulation, malaria episodes and parasitemia with and without symptoms at time of the serosurvey did not influence testing... This finding is important because commercially available test kits have only been evaluated in Europeans not exposed to parasites... We cannot completely rule out the possibility that other infectious agents, which are either only prevalent or more prevalent in African populations, could have resulted in false-positive results.

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Seroprevalence of immunoglobulin (Ig) G antibodies against Coxiella burnetii phase II tested by microimmunofluorescence assays (IFA). A) Results of serologic tests of children, a cutoff titer of >64 for C. burnetii phase II IgG was applied; B) results of serologic tests of healthy adults (cutoff >64); C) distribution of C. burnetii phase II IgG titers in all positive children; D) distribution of IgG titers in all positive healthy adults.
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Fa: Seroprevalence of immunoglobulin (Ig) G antibodies against Coxiella burnetii phase II tested by microimmunofluorescence assays (IFA). A) Results of serologic tests of children, a cutoff titer of >64 for C. burnetii phase II IgG was applied; B) results of serologic tests of healthy adults (cutoff >64); C) distribution of C. burnetii phase II IgG titers in all positive children; D) distribution of IgG titers in all positive healthy adults.


Q fever in young children, Ghana.

Kobbe R, Kramme S, Kreuels B, Adjei S, Kreuzberg C, Panning M, Adjei O, Fleischer B, May J - Emerging Infect. Dis. (2008)

Seroprevalence of immunoglobulin (Ig) G antibodies against Coxiella burnetii phase II tested by microimmunofluorescence assays (IFA). A) Results of serologic tests of children, a cutoff titer of >64 for C. burnetii phase II IgG was applied; B) results of serologic tests of healthy adults (cutoff >64); C) distribution of C. burnetii phase II IgG titers in all positive children; D) distribution of IgG titers in all positive healthy adults.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2630046&req=5

Fa: Seroprevalence of immunoglobulin (Ig) G antibodies against Coxiella burnetii phase II tested by microimmunofluorescence assays (IFA). A) Results of serologic tests of children, a cutoff titer of >64 for C. burnetii phase II IgG was applied; B) results of serologic tests of healthy adults (cutoff >64); C) distribution of C. burnetii phase II IgG titers in all positive children; D) distribution of IgG titers in all positive healthy adults.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

To identify all children with Q fever titers, we regarded the following as positive fluorescence reactions to plasma dilutions: >1:64 for phase II immunoglobulin (Ig) G and >1:24 for phase II IgM with sensitivity (specificity) of 97.2% (100%) and 100% (56.3%), respectively... IgM testing was only performed on IgG-positive children... On the day of the serosurvey 71 (32.4%) of 219 children had fever (measured body temperature >38°C or reported fever within the previous 48 hours)... Test outcome did not appear to be influenced by P. falciparum infection, since 4 of 37 IgG-positive children (23 of 182 IgG-negative children) had clinical malaria, 11/37 (62/182) had asymptomatic parasitemia, and 6/37 (38/182) had fever without parasitemia, and there were no significant differences between groups... The frequency of prior malaria episodes also did not influence antibody response... Three aparasitemic children had positive phase II IgM titers (24, 96, and 1,536; phase II IgG 64, 64, and 4,096, respectively)... The child with the high IgM and IgG titers was clinically ill with nonsevere C. burnetii pneumonia... This child was among 10 (27%) of 37 phase II IgG-positive children with detectable anti–C. burnetii phase I antibodies... Children, especially those of illiterate mothers, could also be more frequently exposed to the pathogen... Consumption of unpasteurized dairy products can result in infection or seroconversion without clinical disease... However, because consumption of raw milk in the Ashanti region is regarded as being uncommon by local health authorities, we consider dairy products an unlikely source of the disease... Although participants were intensively exposed to P. falciparum, which causes polyclonal B-cell stimulation, malaria episodes and parasitemia with and without symptoms at time of the serosurvey did not influence testing... This finding is important because commercially available test kits have only been evaluated in Europeans not exposed to parasites... We cannot completely rule out the possibility that other infectious agents, which are either only prevalent or more prevalent in African populations, could have resulted in false-positive results.

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