Limits...
Isolation of novel adenovirus from fruit bat (Pteropus dasymallus yayeyamae).

Maeda K, Hondo E, Terakawa J, Kiso Y, Nakaichi N, Endoh D, Sakai K, Morikawa S, Mizutani T - Emerging Infect. Dis. (2008)

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

To identify the virus, RV1, we applied the rapid determination of viral RNA (RDV) system version 1.0... For detection of viral DNA, we developed a system for rapid determination of viral DNA sequences (RDV-D) by minor modification to the RDV system for RNA viruses... The results indicated that 2 of the fragments were homologous to the gene encoding the precursor of terminal protein (pTP) of adenoviruses... AB302971) were homologous to the gene encoding the precursor of protein VI (pVI) of adenoviruses... These results indicated that RV1 must belong to the family Adenoviridae... To further confirm that RV1 isolate was an adenovirus, we used PCR and sequencing... We performed the first reaction with the outer primer pair (polFouter and polRouter) of a nested PCR method, targeting the viral DNA polymerase gene with highly degenerate consensus primers that have been described recently... A fragment of ≈550 bp was amplified from RV1 as well as from human adenoviruses-1, -3, -4, and -7 (data not shown)... AB303301) showed that RV1 was homologous to tree shrew adenovirus 1 (70.0% amino acid sequence identity), porcine adenovirus 5 (69.2%), canine adenovirus 1 (68.9%), human adenoviruses-3, -16, -21 and -50 (68.9%), and other viruses (>64.8%) in genus Mastadenovirus, but less homologous (46.7%–57.8%) to viruses in other genuses, Siadenovirus, Aviadenovirus, and Atadenovirus... Restriction endonuclease analysis of the RV1 genome indicated that the genome was ≈20–30 kbp (data not shown)... These features are consistent with RV1 being an adenovirus... The isolation of the novel adenovirus seems to be possible because of usage of the primary cells originated from the host; DNA viruses might have more restricted host range than RNA viruses and require host-originated cells for the growth... In addition, our success in DNA virus isolation might have resulted from usage of the adult animal latently and persistently infected with DNA viruses such as adenovirus and herpesvirus.

Show MeSH

Related in: MedlinePlus

Phylogeny of adenoviruses based on analysis of partial amino acid sequences of DNA polymerase protein. Trees were estimated by using the neighbor-joining method based on the amino acid pairwise distance and MEGA 4.0 software (www.megasoftware.net). Numbers represent percentage bootstrap support (100 replicates).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2630045&req=5

Figure 1: Phylogeny of adenoviruses based on analysis of partial amino acid sequences of DNA polymerase protein. Trees were estimated by using the neighbor-joining method based on the amino acid pairwise distance and MEGA 4.0 software (www.megasoftware.net). Numbers represent percentage bootstrap support (100 replicates).

Mentions: To further confirm that RV1 isolate was an adenovirus, we used PCR and sequencing. We performed the first reaction with the outer primer pair (polFouter and polRouter) of a nested PCR method, targeting the viral DNA polymerase gene with highly degenerate consensus primers that have been described recently (5). A fragment of ≈550 bp was amplified from RV1 as well as from human adenoviruses-1, -3, -4, and -7 (data not shown). Sequence analysis of the amplified product (DDBJ/EMBL/GenBank accession no. AB303301) showed that RV1 was homologous to tree shrew adenovirus 1 (70.0% amino acid sequence identity), porcine adenovirus 5 (69.2%), canine adenovirus 1 (68.9%), human adenoviruses-3, -16, -21 and -50 (68.9%), and other viruses (>64.8%) in genus Mastadenovirus, but less homologous (46.7%–57.8%) to viruses in other genuses, Siadenovirus, Aviadenovirus, and Atadenovirus. In addition, a phylogenic tree based on amino acid sequences indicated that RV1 belongs to family Adenoviridae, genus Mastadenovirus (Figure).


Isolation of novel adenovirus from fruit bat (Pteropus dasymallus yayeyamae).

Maeda K, Hondo E, Terakawa J, Kiso Y, Nakaichi N, Endoh D, Sakai K, Morikawa S, Mizutani T - Emerging Infect. Dis. (2008)

Phylogeny of adenoviruses based on analysis of partial amino acid sequences of DNA polymerase protein. Trees were estimated by using the neighbor-joining method based on the amino acid pairwise distance and MEGA 4.0 software (www.megasoftware.net). Numbers represent percentage bootstrap support (100 replicates).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2630045&req=5

Figure 1: Phylogeny of adenoviruses based on analysis of partial amino acid sequences of DNA polymerase protein. Trees were estimated by using the neighbor-joining method based on the amino acid pairwise distance and MEGA 4.0 software (www.megasoftware.net). Numbers represent percentage bootstrap support (100 replicates).
Mentions: To further confirm that RV1 isolate was an adenovirus, we used PCR and sequencing. We performed the first reaction with the outer primer pair (polFouter and polRouter) of a nested PCR method, targeting the viral DNA polymerase gene with highly degenerate consensus primers that have been described recently (5). A fragment of ≈550 bp was amplified from RV1 as well as from human adenoviruses-1, -3, -4, and -7 (data not shown). Sequence analysis of the amplified product (DDBJ/EMBL/GenBank accession no. AB303301) showed that RV1 was homologous to tree shrew adenovirus 1 (70.0% amino acid sequence identity), porcine adenovirus 5 (69.2%), canine adenovirus 1 (68.9%), human adenoviruses-3, -16, -21 and -50 (68.9%), and other viruses (>64.8%) in genus Mastadenovirus, but less homologous (46.7%–57.8%) to viruses in other genuses, Siadenovirus, Aviadenovirus, and Atadenovirus. In addition, a phylogenic tree based on amino acid sequences indicated that RV1 belongs to family Adenoviridae, genus Mastadenovirus (Figure).

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

To identify the virus, RV1, we applied the rapid determination of viral RNA (RDV) system version 1.0... For detection of viral DNA, we developed a system for rapid determination of viral DNA sequences (RDV-D) by minor modification to the RDV system for RNA viruses... The results indicated that 2 of the fragments were homologous to the gene encoding the precursor of terminal protein (pTP) of adenoviruses... AB302971) were homologous to the gene encoding the precursor of protein VI (pVI) of adenoviruses... These results indicated that RV1 must belong to the family Adenoviridae... To further confirm that RV1 isolate was an adenovirus, we used PCR and sequencing... We performed the first reaction with the outer primer pair (polFouter and polRouter) of a nested PCR method, targeting the viral DNA polymerase gene with highly degenerate consensus primers that have been described recently... A fragment of ≈550 bp was amplified from RV1 as well as from human adenoviruses-1, -3, -4, and -7 (data not shown)... AB303301) showed that RV1 was homologous to tree shrew adenovirus 1 (70.0% amino acid sequence identity), porcine adenovirus 5 (69.2%), canine adenovirus 1 (68.9%), human adenoviruses-3, -16, -21 and -50 (68.9%), and other viruses (>64.8%) in genus Mastadenovirus, but less homologous (46.7%–57.8%) to viruses in other genuses, Siadenovirus, Aviadenovirus, and Atadenovirus... Restriction endonuclease analysis of the RV1 genome indicated that the genome was ≈20–30 kbp (data not shown)... These features are consistent with RV1 being an adenovirus... The isolation of the novel adenovirus seems to be possible because of usage of the primary cells originated from the host; DNA viruses might have more restricted host range than RNA viruses and require host-originated cells for the growth... In addition, our success in DNA virus isolation might have resulted from usage of the adult animal latently and persistently infected with DNA viruses such as adenovirus and herpesvirus.

Show MeSH
Related in: MedlinePlus