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Differences in the histopathology and matrix metalloproteinase expression in Tenon's tissue of primary open-angle glaucoma and primary angle-closure glaucoma.

Seo JH, Park KH, Kim YJ, Yoo YC, Kang SH, Kim DM - Korean J Ophthalmol (2008)

Bottom Line: The number and instillation period of topical eye drops were reviewed.H&E and Masson's trichrome staining and immunohistochemistry for MMP-1, MMP-2, and MMP-9 were performed.The MMP expression was altered in the Tenon's tissue of glaucoma patients compared to the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: To investigate the differences in the histopathology and matrix metalloproteinase (MMP) expression in the Tenon's tissue of primary open-angle glaucoma (POAG) patients, primary angle-closure glaucoma (PACG) patients, and non-glaucomatous patients.

Methods: POAG and PACG patients, who underwent a trabeculectomy and had no history of ocular disease except glaucoma, were enrolled. The number and instillation period of topical eye drops were reviewed. For the controls, which were patients without glaucoma or a history of ocular surgery, the Tenon's tissue was obtained in the course of retinal detachment surgery. For glaucoma patients, the Tenon's tissue was obtained during the trabeculectomy. H&E and Masson's trichrome staining and immunohistochemistry for MMP-1, MMP-2, and MMP-9 were performed. A total of six eyes of POAG, six eyes of PACG, and four control eyes were evaluated.

Results: The duration of topical anti-glaucoma medication and the mean number of anti-glaucoma medications were similar in the POAG and PACG groups. The levels of MMP-1 and 2 were elevated in the POAG and PACG groups compared to the control group (p=0.03, 0.01, respectively). Compared with the control group, the MMP-2 level was higher in the POAG patients (p=0.01), whereas the MMP-1 was higher in the PACG patients (p=0.04). The levels of MMP-9 in the POAG and PACG patients were not significantly different from that of the control patients (p=0.48, 0.26). The levels of MMP-2 were significantly lower in the PACG patients than in the POAG patients (p=0.02).

Conclusions: The MMP expression was altered in the Tenon's tissue of glaucoma patients compared to the control group. The levels of MMP-2 were lower in the PACG patients than in the POAG patients. These results suggest that there may be histopathological differences in the Tenon's tissue of POAG and PACG patients.

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H&E stain, Masson's Trichrome stain, and immunohistochemistry of the Tenon's tissue of the control group (A), POAG group (B), PACG group (C), Positive Control (D: adenocarcinoma of colon), Negative Control (E: sections were prepared with PBS instead of primary antibody MMP-1, 2, and 9) (magnification ×200)The Tenon's tissue of both the POAG and the PACG patients under H&E and Masson's trichrome staining showed higher subepithelial collagen and fibroblast density than that of the controls. The fibrosis under H&E and Masson's trichrome staining appeared to be more advanced in the POAG patients than in the PACG patients (arrow). Compared with the controls, the level of MMP-1 and 2 were higher in the POAG patients (grade 2, 3, respectively). The expression of MMP-2 was lower in the PACG patients than the POAG patients. The level of MMP-9 was less in POAG and PACG patients than the controls.
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Figure 1: H&E stain, Masson's Trichrome stain, and immunohistochemistry of the Tenon's tissue of the control group (A), POAG group (B), PACG group (C), Positive Control (D: adenocarcinoma of colon), Negative Control (E: sections were prepared with PBS instead of primary antibody MMP-1, 2, and 9) (magnification ×200)The Tenon's tissue of both the POAG and the PACG patients under H&E and Masson's trichrome staining showed higher subepithelial collagen and fibroblast density than that of the controls. The fibrosis under H&E and Masson's trichrome staining appeared to be more advanced in the POAG patients than in the PACG patients (arrow). Compared with the controls, the level of MMP-1 and 2 were higher in the POAG patients (grade 2, 3, respectively). The expression of MMP-2 was lower in the PACG patients than the POAG patients. The level of MMP-9 was less in POAG and PACG patients than the controls.

Mentions: The mean age of the POAG and PACG patients at the time of surgery was 59.0±14.6 years and 68.3±4.5 years, respectively (p=0.31). The preoperative IOP in the POAG and PACG patients was 22.2±4.4 mmHg and 27.5±17.3 mmHg, respectively (p=0.48) The mean age of the control patients at the time of surgery was 53.0±13.1 and their mean preoperative IOP was 13.5±1.3 mmHg (Table 2). The Tenon's tissues of both the POAG and the PACG patients showed significant increases in the subepithelial collagen and fibroblast density under H&E and Masson's trichrome staining. The fibrosis appeared to be more advanced in the POAG patients than in the PACG patients under H&E and Masson's trichrome staining (Fig. 1). The levels of MMP-1 and 2 were elevated in the POAG and PACG groups compared to the control group (p=0.03 and 0.01, respectively, Table 3). However, the level of MMP-9 was not significantly different between the glaucoma groups and control group (p=0.86). Comparing the POAG group and the control group, the level of MMP-2 was higher in the POAG patients (p=0.01), while the levels of MMP-1 and 9 were not different (p=0.11, and 0.48, respectively). Also, comparing the PACG group and control group, the level of MMP-1 was higher in the PACG group (p=0.04), while the levels of MMP-2 and 9 in the PACG group and the control group were not significantly different (p=0.11, and 0.26). Furthermore, comparing POAG and PACG, the level of MMP-2 was significantly lower in the PACG patients than in the POAG patients (p=0.02); however, the levels of MMP-1 and 9 were not significantly different between them (p=0.40, and 0.24).


Differences in the histopathology and matrix metalloproteinase expression in Tenon's tissue of primary open-angle glaucoma and primary angle-closure glaucoma.

Seo JH, Park KH, Kim YJ, Yoo YC, Kang SH, Kim DM - Korean J Ophthalmol (2008)

H&E stain, Masson's Trichrome stain, and immunohistochemistry of the Tenon's tissue of the control group (A), POAG group (B), PACG group (C), Positive Control (D: adenocarcinoma of colon), Negative Control (E: sections were prepared with PBS instead of primary antibody MMP-1, 2, and 9) (magnification ×200)The Tenon's tissue of both the POAG and the PACG patients under H&E and Masson's trichrome staining showed higher subepithelial collagen and fibroblast density than that of the controls. The fibrosis under H&E and Masson's trichrome staining appeared to be more advanced in the POAG patients than in the PACG patients (arrow). Compared with the controls, the level of MMP-1 and 2 were higher in the POAG patients (grade 2, 3, respectively). The expression of MMP-2 was lower in the PACG patients than the POAG patients. The level of MMP-9 was less in POAG and PACG patients than the controls.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2629951&req=5

Figure 1: H&E stain, Masson's Trichrome stain, and immunohistochemistry of the Tenon's tissue of the control group (A), POAG group (B), PACG group (C), Positive Control (D: adenocarcinoma of colon), Negative Control (E: sections were prepared with PBS instead of primary antibody MMP-1, 2, and 9) (magnification ×200)The Tenon's tissue of both the POAG and the PACG patients under H&E and Masson's trichrome staining showed higher subepithelial collagen and fibroblast density than that of the controls. The fibrosis under H&E and Masson's trichrome staining appeared to be more advanced in the POAG patients than in the PACG patients (arrow). Compared with the controls, the level of MMP-1 and 2 were higher in the POAG patients (grade 2, 3, respectively). The expression of MMP-2 was lower in the PACG patients than the POAG patients. The level of MMP-9 was less in POAG and PACG patients than the controls.
Mentions: The mean age of the POAG and PACG patients at the time of surgery was 59.0±14.6 years and 68.3±4.5 years, respectively (p=0.31). The preoperative IOP in the POAG and PACG patients was 22.2±4.4 mmHg and 27.5±17.3 mmHg, respectively (p=0.48) The mean age of the control patients at the time of surgery was 53.0±13.1 and their mean preoperative IOP was 13.5±1.3 mmHg (Table 2). The Tenon's tissues of both the POAG and the PACG patients showed significant increases in the subepithelial collagen and fibroblast density under H&E and Masson's trichrome staining. The fibrosis appeared to be more advanced in the POAG patients than in the PACG patients under H&E and Masson's trichrome staining (Fig. 1). The levels of MMP-1 and 2 were elevated in the POAG and PACG groups compared to the control group (p=0.03 and 0.01, respectively, Table 3). However, the level of MMP-9 was not significantly different between the glaucoma groups and control group (p=0.86). Comparing the POAG group and the control group, the level of MMP-2 was higher in the POAG patients (p=0.01), while the levels of MMP-1 and 9 were not different (p=0.11, and 0.48, respectively). Also, comparing the PACG group and control group, the level of MMP-1 was higher in the PACG group (p=0.04), while the levels of MMP-2 and 9 in the PACG group and the control group were not significantly different (p=0.11, and 0.26). Furthermore, comparing POAG and PACG, the level of MMP-2 was significantly lower in the PACG patients than in the POAG patients (p=0.02); however, the levels of MMP-1 and 9 were not significantly different between them (p=0.40, and 0.24).

Bottom Line: The number and instillation period of topical eye drops were reviewed.H&E and Masson's trichrome staining and immunohistochemistry for MMP-1, MMP-2, and MMP-9 were performed.The MMP expression was altered in the Tenon's tissue of glaucoma patients compared to the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: To investigate the differences in the histopathology and matrix metalloproteinase (MMP) expression in the Tenon's tissue of primary open-angle glaucoma (POAG) patients, primary angle-closure glaucoma (PACG) patients, and non-glaucomatous patients.

Methods: POAG and PACG patients, who underwent a trabeculectomy and had no history of ocular disease except glaucoma, were enrolled. The number and instillation period of topical eye drops were reviewed. For the controls, which were patients without glaucoma or a history of ocular surgery, the Tenon's tissue was obtained in the course of retinal detachment surgery. For glaucoma patients, the Tenon's tissue was obtained during the trabeculectomy. H&E and Masson's trichrome staining and immunohistochemistry for MMP-1, MMP-2, and MMP-9 were performed. A total of six eyes of POAG, six eyes of PACG, and four control eyes were evaluated.

Results: The duration of topical anti-glaucoma medication and the mean number of anti-glaucoma medications were similar in the POAG and PACG groups. The levels of MMP-1 and 2 were elevated in the POAG and PACG groups compared to the control group (p=0.03, 0.01, respectively). Compared with the control group, the MMP-2 level was higher in the POAG patients (p=0.01), whereas the MMP-1 was higher in the PACG patients (p=0.04). The levels of MMP-9 in the POAG and PACG patients were not significantly different from that of the control patients (p=0.48, 0.26). The levels of MMP-2 were significantly lower in the PACG patients than in the POAG patients (p=0.02).

Conclusions: The MMP expression was altered in the Tenon's tissue of glaucoma patients compared to the control group. The levels of MMP-2 were lower in the PACG patients than in the POAG patients. These results suggest that there may be histopathological differences in the Tenon's tissue of POAG and PACG patients.

Show MeSH
Related in: MedlinePlus