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Short-term Efficacy of Topical Immunosuppressive Agents on the Survival of Cultivated Allo-Conjunctival Equivalents.

Shin YJ, Kim MK, Oh JY, Wee WR, Lee JH, Ko JH, Lee HJ, Lee JL, Min BM, Sohn YS - Korean J Ophthalmol (2008)

Bottom Line: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes.In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7.Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Dongguk University College of Medicine, Goyang, Korea.

ABSTRACT

Purpose: To investigate the short-term efficacy of topical immunosuppressive agents on the survival of cultivated allo-conjunctival equivalents.

Methods: Twenty-five eyes of New Zealand white rabbits were included. Temporal conjunctivae were trephined to a diameter of 7.5 mm, and then cultured allo-conjunctival epithelial cells on amniotic membrane were transplanted onto them. Various immunosuppressants including steroid, cyclosporine, and rapamycin were applied topically four times a day for a week. Epithelial defects and graft edema were graded daily. Numbers of inflammatory cells were measured in H&E. PKH26 and cytokeratin 4 and 7 were immunostained.

Results: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes. In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7. Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes.

Conclusions: Topical steroid or rapamycin can help to suppress acute inflammation and enhance the acute survival of transplanted conjunctival cells.

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Related in: MedlinePlus

PKH26 positive cells as transplanted donor cells in untreated (A), and in 0.5% cyclosporine (B), 1% steroid (C), 0.01% rapamycin (D) treated eyes in the cultivated conjunctival equivalent transplanted group. Hoechst staining in steroid (E) or 0.01% rapamycin (F) treated eyes in the cultivated conjunctival equivalent transplanted group. Magnification; ×200.
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Figure 4: PKH26 positive cells as transplanted donor cells in untreated (A), and in 0.5% cyclosporine (B), 1% steroid (C), 0.01% rapamycin (D) treated eyes in the cultivated conjunctival equivalent transplanted group. Hoechst staining in steroid (E) or 0.01% rapamycin (F) treated eyes in the cultivated conjunctival equivalent transplanted group. Magnification; ×200.

Mentions: PKH26 staining showed no donor cells in the control without any immune suppressant or in 0.5% CsA treated eyes (Fig. 4A, B). PKH26 positive cells were found in 1% steroid, 0.01% rapamycin applied eyes (Fig. 4C, D), and 1% steroid or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7 (Fig. 5A~D). We confirmed the cells using a DNA-binding dye Hoechst staining (Fig. 4E, F). Taken together, treatment with topical steroid or rapamycin enhanced grafted epithelial cell survival.


Short-term Efficacy of Topical Immunosuppressive Agents on the Survival of Cultivated Allo-Conjunctival Equivalents.

Shin YJ, Kim MK, Oh JY, Wee WR, Lee JH, Ko JH, Lee HJ, Lee JL, Min BM, Sohn YS - Korean J Ophthalmol (2008)

PKH26 positive cells as transplanted donor cells in untreated (A), and in 0.5% cyclosporine (B), 1% steroid (C), 0.01% rapamycin (D) treated eyes in the cultivated conjunctival equivalent transplanted group. Hoechst staining in steroid (E) or 0.01% rapamycin (F) treated eyes in the cultivated conjunctival equivalent transplanted group. Magnification; ×200.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2629933&req=5

Figure 4: PKH26 positive cells as transplanted donor cells in untreated (A), and in 0.5% cyclosporine (B), 1% steroid (C), 0.01% rapamycin (D) treated eyes in the cultivated conjunctival equivalent transplanted group. Hoechst staining in steroid (E) or 0.01% rapamycin (F) treated eyes in the cultivated conjunctival equivalent transplanted group. Magnification; ×200.
Mentions: PKH26 staining showed no donor cells in the control without any immune suppressant or in 0.5% CsA treated eyes (Fig. 4A, B). PKH26 positive cells were found in 1% steroid, 0.01% rapamycin applied eyes (Fig. 4C, D), and 1% steroid or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7 (Fig. 5A~D). We confirmed the cells using a DNA-binding dye Hoechst staining (Fig. 4E, F). Taken together, treatment with topical steroid or rapamycin enhanced grafted epithelial cell survival.

Bottom Line: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes.In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7.Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Dongguk University College of Medicine, Goyang, Korea.

ABSTRACT

Purpose: To investigate the short-term efficacy of topical immunosuppressive agents on the survival of cultivated allo-conjunctival equivalents.

Methods: Twenty-five eyes of New Zealand white rabbits were included. Temporal conjunctivae were trephined to a diameter of 7.5 mm, and then cultured allo-conjunctival epithelial cells on amniotic membrane were transplanted onto them. Various immunosuppressants including steroid, cyclosporine, and rapamycin were applied topically four times a day for a week. Epithelial defects and graft edema were graded daily. Numbers of inflammatory cells were measured in H&E. PKH26 and cytokeratin 4 and 7 were immunostained.

Results: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes. In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7. Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes.

Conclusions: Topical steroid or rapamycin can help to suppress acute inflammation and enhance the acute survival of transplanted conjunctival cells.

Show MeSH
Related in: MedlinePlus