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Short-term Efficacy of Topical Immunosuppressive Agents on the Survival of Cultivated Allo-Conjunctival Equivalents.

Shin YJ, Kim MK, Oh JY, Wee WR, Lee JH, Ko JH, Lee HJ, Lee JL, Min BM, Sohn YS - Korean J Ophthalmol (2008)

Bottom Line: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes.In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7.Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Dongguk University College of Medicine, Goyang, Korea.

ABSTRACT

Purpose: To investigate the short-term efficacy of topical immunosuppressive agents on the survival of cultivated allo-conjunctival equivalents.

Methods: Twenty-five eyes of New Zealand white rabbits were included. Temporal conjunctivae were trephined to a diameter of 7.5 mm, and then cultured allo-conjunctival epithelial cells on amniotic membrane were transplanted onto them. Various immunosuppressants including steroid, cyclosporine, and rapamycin were applied topically four times a day for a week. Epithelial defects and graft edema were graded daily. Numbers of inflammatory cells were measured in H&E. PKH26 and cytokeratin 4 and 7 were immunostained.

Results: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes. In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7. Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes.

Conclusions: Topical steroid or rapamycin can help to suppress acute inflammation and enhance the acute survival of transplanted conjunctival cells.

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Related in: MedlinePlus

Temporal conjunctivae was trephined to a diameter of 7.5 mm, and cultivated conjunctival equivalent or an allo-conjunctival graft was transplanted.
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Related In: Results  -  Collection

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Figure 1: Temporal conjunctivae was trephined to a diameter of 7.5 mm, and cultivated conjunctival equivalent or an allo-conjunctival graft was transplanted.

Mentions: Cultivated cells on denuded AMs for 2 weeks were used as cultivated conjunctival equivalents. Temporal conjunctivae were trephined to a diameter of 7.5 mm. Cultivated conjunctival equivalents were transplanted to the defected conjunctival lesion in recipients (Fig. 1) and sutured with interrupted and continuous 10.0 nylon. AMs were overlaid using 10.0 nylon to help graft stability.


Short-term Efficacy of Topical Immunosuppressive Agents on the Survival of Cultivated Allo-Conjunctival Equivalents.

Shin YJ, Kim MK, Oh JY, Wee WR, Lee JH, Ko JH, Lee HJ, Lee JL, Min BM, Sohn YS - Korean J Ophthalmol (2008)

Temporal conjunctivae was trephined to a diameter of 7.5 mm, and cultivated conjunctival equivalent or an allo-conjunctival graft was transplanted.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2629933&req=5

Figure 1: Temporal conjunctivae was trephined to a diameter of 7.5 mm, and cultivated conjunctival equivalent or an allo-conjunctival graft was transplanted.
Mentions: Cultivated cells on denuded AMs for 2 weeks were used as cultivated conjunctival equivalents. Temporal conjunctivae were trephined to a diameter of 7.5 mm. Cultivated conjunctival equivalents were transplanted to the defected conjunctival lesion in recipients (Fig. 1) and sutured with interrupted and continuous 10.0 nylon. AMs were overlaid using 10.0 nylon to help graft stability.

Bottom Line: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes.In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7.Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Dongguk University College of Medicine, Goyang, Korea.

ABSTRACT

Purpose: To investigate the short-term efficacy of topical immunosuppressive agents on the survival of cultivated allo-conjunctival equivalents.

Methods: Twenty-five eyes of New Zealand white rabbits were included. Temporal conjunctivae were trephined to a diameter of 7.5 mm, and then cultured allo-conjunctival epithelial cells on amniotic membrane were transplanted onto them. Various immunosuppressants including steroid, cyclosporine, and rapamycin were applied topically four times a day for a week. Epithelial defects and graft edema were graded daily. Numbers of inflammatory cells were measured in H&E. PKH26 and cytokeratin 4 and 7 were immunostained.

Results: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes. In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7. Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes.

Conclusions: Topical steroid or rapamycin can help to suppress acute inflammation and enhance the acute survival of transplanted conjunctival cells.

Show MeSH
Related in: MedlinePlus