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Transforming growth factor-beta does not induce endothelin-1 secretion in primary cultured human tenon's fibroblasts.

Hong S, Kim CY, Lee JB, Yoko I, Seong GJ - Korean J Ophthalmol (2008)

View Article: PubMed Central - PubMed

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Subconjunctival fibrosis is closely related to scarring after glaucoma filtering surgery, and transforming growth factor-β (TGF-β) is known to play a crucial role in this scarring process... Even though fibroblasts usually are not a major source of ET-1, these different cellular responses may implicate the characteristics of Tenon's fibroblasts as being different to fibroblasts from other tissue... Studies concerning other intracellular signaling pathways associated with pro- and anti- fibrosis are also needed to confirm the similarities of fibroblasts from human Tenon's capsule with that from other organs... For several decades, the mechanism associated with subconjunctival fibrosis has been a matter of great concern to glaucoma specialists because scarring affects the outcome after glaucoma filtering surgery... Transforming growth factor-β (TGF-β) is known to play an important role in this scarring process, and many in vitro and in vivo studies reveal topical anti-TGF-β antibodies blocking myofibroblastic transformation of fibroblasts and reducing extracellular matrix (ECM) production in Tenon's capsule. - Fibroblast is a type of cell that synthesizes and maintains the ECM of various tissues... Antibodies against ET-1, collagen type I, fibronectin, and β-actin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA)... Intensity of each band was calculated as compared with that of 0 hr time point, and all procedures were performed in triplicate... This increase was more apparent after 12 hours... However, a band that represents ET-1 was hardly detected on all samples and it did not show any significant change... Beta-actin, an internal control, also was not changed... Our results demonstrate that TGF-β1 has no influence on the synthesis of ET-1 in human Tenon's fibroblasts and the fibroblasts of human Tenon's capsule seem to have no autocrine function for the ET-1... Even though fibroblasts usually are not a major source of ET-1 even in other extraocular tissues, these different cellular responses may implicate that characteristics of Tenon's fibroblasts are different from fibroblasts of other tissue... Further study about their response to exogenous ET-1 is required and other intracellular signaling pathways associated with pro- and anti- fibrotic courses are also needed to be confirmed directly... Understanding the precise mechanisms of the fibrotic process may give promise to more successful glaucoma filtering surgery.

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Representative Western immunoblots. (A) collagen type I, (B) fibronectin, (C) endothelin-1, and (D) beta-actin.
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Figure 1: Representative Western immunoblots. (A) collagen type I, (B) fibronectin, (C) endothelin-1, and (D) beta-actin.

Mentions: Representative Western immunoblots are shown at Figure 1 and the intensity data are demonstrated at Figure 2. As compared with control (at 0 hr), the synthesis of collagen type I and fibronectin increased with time by more than 3 times. This increase was more apparent after 12 hours. However, a band that represents ET-1 was hardly detected on all samples and it did not show any significant change. Beta-actin, an internal control, also was not changed.


Transforming growth factor-beta does not induce endothelin-1 secretion in primary cultured human tenon's fibroblasts.

Hong S, Kim CY, Lee JB, Yoko I, Seong GJ - Korean J Ophthalmol (2008)

Representative Western immunoblots. (A) collagen type I, (B) fibronectin, (C) endothelin-1, and (D) beta-actin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2629916&req=5

Figure 1: Representative Western immunoblots. (A) collagen type I, (B) fibronectin, (C) endothelin-1, and (D) beta-actin.
Mentions: Representative Western immunoblots are shown at Figure 1 and the intensity data are demonstrated at Figure 2. As compared with control (at 0 hr), the synthesis of collagen type I and fibronectin increased with time by more than 3 times. This increase was more apparent after 12 hours. However, a band that represents ET-1 was hardly detected on all samples and it did not show any significant change. Beta-actin, an internal control, also was not changed.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Subconjunctival fibrosis is closely related to scarring after glaucoma filtering surgery, and transforming growth factor-β (TGF-β) is known to play a crucial role in this scarring process... Even though fibroblasts usually are not a major source of ET-1, these different cellular responses may implicate the characteristics of Tenon's fibroblasts as being different to fibroblasts from other tissue... Studies concerning other intracellular signaling pathways associated with pro- and anti- fibrosis are also needed to confirm the similarities of fibroblasts from human Tenon's capsule with that from other organs... For several decades, the mechanism associated with subconjunctival fibrosis has been a matter of great concern to glaucoma specialists because scarring affects the outcome after glaucoma filtering surgery... Transforming growth factor-β (TGF-β) is known to play an important role in this scarring process, and many in vitro and in vivo studies reveal topical anti-TGF-β antibodies blocking myofibroblastic transformation of fibroblasts and reducing extracellular matrix (ECM) production in Tenon's capsule. - Fibroblast is a type of cell that synthesizes and maintains the ECM of various tissues... Antibodies against ET-1, collagen type I, fibronectin, and β-actin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA)... Intensity of each band was calculated as compared with that of 0 hr time point, and all procedures were performed in triplicate... This increase was more apparent after 12 hours... However, a band that represents ET-1 was hardly detected on all samples and it did not show any significant change... Beta-actin, an internal control, also was not changed... Our results demonstrate that TGF-β1 has no influence on the synthesis of ET-1 in human Tenon's fibroblasts and the fibroblasts of human Tenon's capsule seem to have no autocrine function for the ET-1... Even though fibroblasts usually are not a major source of ET-1 even in other extraocular tissues, these different cellular responses may implicate that characteristics of Tenon's fibroblasts are different from fibroblasts of other tissue... Further study about their response to exogenous ET-1 is required and other intracellular signaling pathways associated with pro- and anti- fibrotic courses are also needed to be confirmed directly... Understanding the precise mechanisms of the fibrotic process may give promise to more successful glaucoma filtering surgery.

Show MeSH