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Variability in interbeat duration influences myocardial contractility in rat cardiac trabeculae.

Torres CA, Varian KD, Janssen PM - Open Cardiovasc Med J (2008)

Bottom Line: Fixed rate response was measured before and after each variable period and average force was calculated.We observed no significant change in force at 4 Hz (n=17), and 6 Hz (n=6) between fixed and variable pacing but observed a significant, 10% increase in contractile strength at 8 Hz (from 15.1 to 16.5 mN/mm(2), p<0.05, n=6).Our results show that under certain conditions, by simply introducing variation in the beat-to-beat duration without affecting the number of beats per minute, a positive inotropic effect with corresponding changes in the calcium transients can be generated.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Cell Biology, and Department of Emergency Medicine, 304 Hamilton Hall, 1645 Neil Avenue, The Ohio State University, Columbus, OH, USA.

ABSTRACT
There is an intense search for positive inotropic strategies. It is well known that the interbeat duration is a critical determinant of cardiac contractility. Generally, when frequency increases, so does contractile strength. We hypothesize that the beat-to-beat variability at a given heart rate also modulates cardiac contractility. To test this hypothesis, thin, uniform rat cardiac trabeculae were isolated from the right ventricle and stimulated to isometrically contract, alternating between fixed steady state versus variable inter-beat intervals (same total number of beats in each period). Trabeculae were stimulated at 4 Hz with interbeat variation between 20 and 120% (n=17). In a second series of experiments trabeculae were stimulated at 3 different physiologic frequencies with a 40% interbeat variation. Fixed rate response was measured before and after each variable period and average force was calculated. In order to investigate the mechanism underlying the changes in contractility we used iontophoretically loaded bis-fura-2 salt to monitor intracellular calcium transients. We observed no significant change in force at 4 Hz (n=17), and 6 Hz (n=6) between fixed and variable pacing but observed a significant, 10% increase in contractile strength at 8 Hz (from 15.1 to 16.5 mN/mm(2), p<0.05, n=6). Our results show that under certain conditions, by simply introducing variation in the beat-to-beat duration without affecting the number of beats per minute, a positive inotropic effect with corresponding changes in the calcium transients can be generated.

No MeSH data available.


Related in: MedlinePlus

Force development is dependent on duration of the pre-beat interval; when previous beat-duration increases, so does peak twitch force development (Fdev). In the depicted example, at a frequency of 8 Hz a 40% variation was imposed. Thus, inter-beat duration varied from 100 to 150 ms, with an average of 125 ms (=8 Hz).
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Figure 1: Force development is dependent on duration of the pre-beat interval; when previous beat-duration increases, so does peak twitch force development (Fdev). In the depicted example, at a frequency of 8 Hz a 40% variation was imposed. Thus, inter-beat duration varied from 100 to 150 ms, with an average of 125 ms (=8 Hz).

Mentions: In Fig. (1) we demonstrate that when pre-beat duration increased, so did contractile strength. In the depicted example, at a frequency of 8 Hz (cycle time of 125 ms), a 40% variation was imposed, making the shortest beat-to-beat interval 100 ms, and the longest interval 150 ms. The highest contractile force was observed after the longest pre-beat duration (i.e. after the longest diastolic interval), whereas the shortest intervals were followed by the weakest beats.


Variability in interbeat duration influences myocardial contractility in rat cardiac trabeculae.

Torres CA, Varian KD, Janssen PM - Open Cardiovasc Med J (2008)

Force development is dependent on duration of the pre-beat interval; when previous beat-duration increases, so does peak twitch force development (Fdev). In the depicted example, at a frequency of 8 Hz a 40% variation was imposed. Thus, inter-beat duration varied from 100 to 150 ms, with an average of 125 ms (=8 Hz).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2627530&req=5

Figure 1: Force development is dependent on duration of the pre-beat interval; when previous beat-duration increases, so does peak twitch force development (Fdev). In the depicted example, at a frequency of 8 Hz a 40% variation was imposed. Thus, inter-beat duration varied from 100 to 150 ms, with an average of 125 ms (=8 Hz).
Mentions: In Fig. (1) we demonstrate that when pre-beat duration increased, so did contractile strength. In the depicted example, at a frequency of 8 Hz (cycle time of 125 ms), a 40% variation was imposed, making the shortest beat-to-beat interval 100 ms, and the longest interval 150 ms. The highest contractile force was observed after the longest pre-beat duration (i.e. after the longest diastolic interval), whereas the shortest intervals were followed by the weakest beats.

Bottom Line: Fixed rate response was measured before and after each variable period and average force was calculated.We observed no significant change in force at 4 Hz (n=17), and 6 Hz (n=6) between fixed and variable pacing but observed a significant, 10% increase in contractile strength at 8 Hz (from 15.1 to 16.5 mN/mm(2), p<0.05, n=6).Our results show that under certain conditions, by simply introducing variation in the beat-to-beat duration without affecting the number of beats per minute, a positive inotropic effect with corresponding changes in the calcium transients can be generated.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Cell Biology, and Department of Emergency Medicine, 304 Hamilton Hall, 1645 Neil Avenue, The Ohio State University, Columbus, OH, USA.

ABSTRACT
There is an intense search for positive inotropic strategies. It is well known that the interbeat duration is a critical determinant of cardiac contractility. Generally, when frequency increases, so does contractile strength. We hypothesize that the beat-to-beat variability at a given heart rate also modulates cardiac contractility. To test this hypothesis, thin, uniform rat cardiac trabeculae were isolated from the right ventricle and stimulated to isometrically contract, alternating between fixed steady state versus variable inter-beat intervals (same total number of beats in each period). Trabeculae were stimulated at 4 Hz with interbeat variation between 20 and 120% (n=17). In a second series of experiments trabeculae were stimulated at 3 different physiologic frequencies with a 40% interbeat variation. Fixed rate response was measured before and after each variable period and average force was calculated. In order to investigate the mechanism underlying the changes in contractility we used iontophoretically loaded bis-fura-2 salt to monitor intracellular calcium transients. We observed no significant change in force at 4 Hz (n=17), and 6 Hz (n=6) between fixed and variable pacing but observed a significant, 10% increase in contractile strength at 8 Hz (from 15.1 to 16.5 mN/mm(2), p<0.05, n=6). Our results show that under certain conditions, by simply introducing variation in the beat-to-beat duration without affecting the number of beats per minute, a positive inotropic effect with corresponding changes in the calcium transients can be generated.

No MeSH data available.


Related in: MedlinePlus