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Synergistic effect of the combination of nanoparticulate Fe3O4 and Au with daunomycin on K562/A02 cells.

Chen BA, Dai YY, Wang XM, Zhang RY, Xu WL, Shen HL, Gao F, Sun Q, Deng XJ, Ding JH, Gao C, Sun YY, Cheng J, Wang J, Zhao G, Chen NN - Int J Nanomedicine (2008)

Bottom Line: The MDR1 gene expression of the K562/A02 cells was also studied by RT-PCR method.Moreover, our observations illustrate that although these two kinds of nanoparticles themselves could not lower the MDRI gene expression of the K562/A02 cells, yet they could degrade the MDR1 gene level when combining with anticancer drug DNR.This raises the possibility to combine the nano-Fe3O4 or nano-Au with DNR to reverse the drug resistance of K562/A02 cells, which could offer a new strategy for the promising efficient chemotherapy of the leukemia patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, P.R. China. cba8888@hotmail.com

ABSTRACT
In this study, we have explored the possibility of the combination of the high reactivity of nano Fe3O4 or Au nanoparticles and daunomycin, one of the most important antitumor drugs in the treatment of acute leukemia clinically, to inhibit MDR of K562/A02 cells. Initially, to determine whether the magnetic nanoparticle Fe3O4 and Au can facilitate the anticancer drug to reverse the resistance of cancer cells, we have explored the cytotoxic effect of daunomycin (DNR) with and without the magnetic nano-Fe3O4 or nano-Au on K562 and K562/A02 cells by MTT assay. Besides, the intracellular DNR concentration and apoptosis of the K562/A02 cells was further investigated by flow cytometry and confocal fluorescence microscopic studies. The MDR1 gene expression of the K562/A02 cells was also studied by RT-PCR method. Our results indicate that 5.0 x 10(-7) M nano-Fe3O4 or 2.0 x 10(-8) M nano-Au is biocompatible and can apparently raise the intracellular DNR accumulation of the K562/A02 cells and increase the apoptosis of tumor cells. Moreover, our observations illustrate that although these two kinds of nanoparticles themselves could not lower the MDRI gene expression of the K562/A02 cells, yet they could degrade the MDR1 gene level when combining with anticancer drug DNR. This raises the possibility to combine the nano-Fe3O4 or nano-Au with DNR to reverse the drug resistance of K562/A02 cells, which could offer a new strategy for the promising efficient chemotherapy of the leukemia patients.

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Related in: MedlinePlus

Effect of nanoparticle on the intracellular accumulation of daunomycin (DNR) in K562/A02 cells (×1000). K562/A02 (A–C) and K562 (D–F) cells were incubated with DNR (50 mg/L) in the absence (A, D) or the presence of Nano-Fe3O4 (5.0×10−7 M) (B, E) or Nano-Au (2.0 × 10−8 M) (C, F) at 37 °C for 30 min. The cells were observed and photographed under a confocal fluorescent microscope.
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f5-ijn-3-343: Effect of nanoparticle on the intracellular accumulation of daunomycin (DNR) in K562/A02 cells (×1000). K562/A02 (A–C) and K562 (D–F) cells were incubated with DNR (50 mg/L) in the absence (A, D) or the presence of Nano-Fe3O4 (5.0×10−7 M) (B, E) or Nano-Au (2.0 × 10−8 M) (C, F) at 37 °C for 30 min. The cells were observed and photographed under a confocal fluorescent microscope.

Mentions: The intracellular DNR concentration of the K562/A02 cells was explored by flow cytometry. Compared with that treated with DNR alone, the intracellular DNR concentration of the K562/A02 cells were found to increase by 71% and 51%, respectively when the K562/A02 cells were treated with DNR in combination with nano-Fe3O4 and nano-Au, respectively. Similar observations were also illustrated through confocal fluorescent microscope (Figure 5). Considering that the anticancer drug daunorubicin is a fluorescent molecule while the relevant nanoparticles have no fluorescence, our observations of the laser confocal fluorescence microscopy further confirm the synergistic effect of these nanoparticles on the cellular uptake of daunorubicin.


Synergistic effect of the combination of nanoparticulate Fe3O4 and Au with daunomycin on K562/A02 cells.

Chen BA, Dai YY, Wang XM, Zhang RY, Xu WL, Shen HL, Gao F, Sun Q, Deng XJ, Ding JH, Gao C, Sun YY, Cheng J, Wang J, Zhao G, Chen NN - Int J Nanomedicine (2008)

Effect of nanoparticle on the intracellular accumulation of daunomycin (DNR) in K562/A02 cells (×1000). K562/A02 (A–C) and K562 (D–F) cells were incubated with DNR (50 mg/L) in the absence (A, D) or the presence of Nano-Fe3O4 (5.0×10−7 M) (B, E) or Nano-Au (2.0 × 10−8 M) (C, F) at 37 °C for 30 min. The cells were observed and photographed under a confocal fluorescent microscope.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2626936&req=5

f5-ijn-3-343: Effect of nanoparticle on the intracellular accumulation of daunomycin (DNR) in K562/A02 cells (×1000). K562/A02 (A–C) and K562 (D–F) cells were incubated with DNR (50 mg/L) in the absence (A, D) or the presence of Nano-Fe3O4 (5.0×10−7 M) (B, E) or Nano-Au (2.0 × 10−8 M) (C, F) at 37 °C for 30 min. The cells were observed and photographed under a confocal fluorescent microscope.
Mentions: The intracellular DNR concentration of the K562/A02 cells was explored by flow cytometry. Compared with that treated with DNR alone, the intracellular DNR concentration of the K562/A02 cells were found to increase by 71% and 51%, respectively when the K562/A02 cells were treated with DNR in combination with nano-Fe3O4 and nano-Au, respectively. Similar observations were also illustrated through confocal fluorescent microscope (Figure 5). Considering that the anticancer drug daunorubicin is a fluorescent molecule while the relevant nanoparticles have no fluorescence, our observations of the laser confocal fluorescence microscopy further confirm the synergistic effect of these nanoparticles on the cellular uptake of daunorubicin.

Bottom Line: The MDR1 gene expression of the K562/A02 cells was also studied by RT-PCR method.Moreover, our observations illustrate that although these two kinds of nanoparticles themselves could not lower the MDRI gene expression of the K562/A02 cells, yet they could degrade the MDR1 gene level when combining with anticancer drug DNR.This raises the possibility to combine the nano-Fe3O4 or nano-Au with DNR to reverse the drug resistance of K562/A02 cells, which could offer a new strategy for the promising efficient chemotherapy of the leukemia patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, P.R. China. cba8888@hotmail.com

ABSTRACT
In this study, we have explored the possibility of the combination of the high reactivity of nano Fe3O4 or Au nanoparticles and daunomycin, one of the most important antitumor drugs in the treatment of acute leukemia clinically, to inhibit MDR of K562/A02 cells. Initially, to determine whether the magnetic nanoparticle Fe3O4 and Au can facilitate the anticancer drug to reverse the resistance of cancer cells, we have explored the cytotoxic effect of daunomycin (DNR) with and without the magnetic nano-Fe3O4 or nano-Au on K562 and K562/A02 cells by MTT assay. Besides, the intracellular DNR concentration and apoptosis of the K562/A02 cells was further investigated by flow cytometry and confocal fluorescence microscopic studies. The MDR1 gene expression of the K562/A02 cells was also studied by RT-PCR method. Our results indicate that 5.0 x 10(-7) M nano-Fe3O4 or 2.0 x 10(-8) M nano-Au is biocompatible and can apparently raise the intracellular DNR accumulation of the K562/A02 cells and increase the apoptosis of tumor cells. Moreover, our observations illustrate that although these two kinds of nanoparticles themselves could not lower the MDRI gene expression of the K562/A02 cells, yet they could degrade the MDR1 gene level when combining with anticancer drug DNR. This raises the possibility to combine the nano-Fe3O4 or nano-Au with DNR to reverse the drug resistance of K562/A02 cells, which could offer a new strategy for the promising efficient chemotherapy of the leukemia patients.

Show MeSH
Related in: MedlinePlus