Limits...
EphrinA I-targeted nanoshells for photothermal ablation of prostate cancer cells.

Gobin AM, Moon JJ, West JL - Int J Nanomedicine (2008)

Bottom Line: In this work, we sought to improve their specificity by targeting them to prostate tumor cells.We report selective targeting of PC-3 cells with nanoshells conjugated to ephrinA I, a ligand for EphA2 receptor that is overexpressed on PC-3 cells.We demonstrate selective photo-thermal destruction of these cells upon application of the NIR laser.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, TX 77251-1892, USA.

ABSTRACT
Gold-coated silica nanoshells are a class of nanoparticles that can be designed to possess strong absorption of light in the near infrared (NIR) wavelength region. When injected intravenously, these nanoshells have been shown to accumulate in tumors and subsequently mediate photothermal treatment, leading to tumor regression. In this work, we sought to improve their specificity by targeting them to prostate tumor cells. We report selective targeting of PC-3 cells with nanoshells conjugated to ephrinA I, a ligand for EphA2 receptor that is overexpressed on PC-3 cells. We demonstrate selective photo-thermal destruction of these cells upon application of the NIR laser.

Show MeSH

Related in: MedlinePlus

Darkfield images of nanoshells bound to cells. A) EphrinAl-nanoshells bound to the PC-3 cells with high expression of EphA2 receptor. B) shows higher magnification of A). C) PEG-nanoshells had minimal binding whereas D) bare nanoshells bound indiscriminately to PC-3 cells and the substrate. E) EphrinAl-nanoshells had minimal binding to HDF. Scale bars in A), C) and E) = 50 μm and B) and D) = 10 μm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2626934&req=5

f4-ijn-3-351: Darkfield images of nanoshells bound to cells. A) EphrinAl-nanoshells bound to the PC-3 cells with high expression of EphA2 receptor. B) shows higher magnification of A). C) PEG-nanoshells had minimal binding whereas D) bare nanoshells bound indiscriminately to PC-3 cells and the substrate. E) EphrinAl-nanoshells had minimal binding to HDF. Scale bars in A), C) and E) = 50 μm and B) and D) = 10 μm.

Mentions: Darkfield microscopy was also used to visualize the nanoshells. Figure 4A shows EphrinAl-nanoshells bound to PC-3 cells as bright spots against the darker background of cell bodies and substrates. Images in higher magnification provide better details of the bound nanoshells (Figure 4B). There was good coverage of EphrinAl-nanoshells over the entire surface of PC-3 cells. PEG-nanoshells did not bind to PC-3 cells (Figure 4C) whereas bare nanoshells bound to both the cells and substrates nonspecifically (Figure 4D). HDF had minimal number of EphrinAl-nanoshells (Figure 4E).


EphrinA I-targeted nanoshells for photothermal ablation of prostate cancer cells.

Gobin AM, Moon JJ, West JL - Int J Nanomedicine (2008)

Darkfield images of nanoshells bound to cells. A) EphrinAl-nanoshells bound to the PC-3 cells with high expression of EphA2 receptor. B) shows higher magnification of A). C) PEG-nanoshells had minimal binding whereas D) bare nanoshells bound indiscriminately to PC-3 cells and the substrate. E) EphrinAl-nanoshells had minimal binding to HDF. Scale bars in A), C) and E) = 50 μm and B) and D) = 10 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2626934&req=5

f4-ijn-3-351: Darkfield images of nanoshells bound to cells. A) EphrinAl-nanoshells bound to the PC-3 cells with high expression of EphA2 receptor. B) shows higher magnification of A). C) PEG-nanoshells had minimal binding whereas D) bare nanoshells bound indiscriminately to PC-3 cells and the substrate. E) EphrinAl-nanoshells had minimal binding to HDF. Scale bars in A), C) and E) = 50 μm and B) and D) = 10 μm.
Mentions: Darkfield microscopy was also used to visualize the nanoshells. Figure 4A shows EphrinAl-nanoshells bound to PC-3 cells as bright spots against the darker background of cell bodies and substrates. Images in higher magnification provide better details of the bound nanoshells (Figure 4B). There was good coverage of EphrinAl-nanoshells over the entire surface of PC-3 cells. PEG-nanoshells did not bind to PC-3 cells (Figure 4C) whereas bare nanoshells bound to both the cells and substrates nonspecifically (Figure 4D). HDF had minimal number of EphrinAl-nanoshells (Figure 4E).

Bottom Line: In this work, we sought to improve their specificity by targeting them to prostate tumor cells.We report selective targeting of PC-3 cells with nanoshells conjugated to ephrinA I, a ligand for EphA2 receptor that is overexpressed on PC-3 cells.We demonstrate selective photo-thermal destruction of these cells upon application of the NIR laser.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, TX 77251-1892, USA.

ABSTRACT
Gold-coated silica nanoshells are a class of nanoparticles that can be designed to possess strong absorption of light in the near infrared (NIR) wavelength region. When injected intravenously, these nanoshells have been shown to accumulate in tumors and subsequently mediate photothermal treatment, leading to tumor regression. In this work, we sought to improve their specificity by targeting them to prostate tumor cells. We report selective targeting of PC-3 cells with nanoshells conjugated to ephrinA I, a ligand for EphA2 receptor that is overexpressed on PC-3 cells. We demonstrate selective photo-thermal destruction of these cells upon application of the NIR laser.

Show MeSH
Related in: MedlinePlus