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EphrinA I-targeted nanoshells for photothermal ablation of prostate cancer cells.

Gobin AM, Moon JJ, West JL - Int J Nanomedicine (2008)

Bottom Line: In this work, we sought to improve their specificity by targeting them to prostate tumor cells.We report selective targeting of PC-3 cells with nanoshells conjugated to ephrinA I, a ligand for EphA2 receptor that is overexpressed on PC-3 cells.We demonstrate selective photo-thermal destruction of these cells upon application of the NIR laser.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, TX 77251-1892, USA.

ABSTRACT
Gold-coated silica nanoshells are a class of nanoparticles that can be designed to possess strong absorption of light in the near infrared (NIR) wavelength region. When injected intravenously, these nanoshells have been shown to accumulate in tumors and subsequently mediate photothermal treatment, leading to tumor regression. In this work, we sought to improve their specificity by targeting them to prostate tumor cells. We report selective targeting of PC-3 cells with nanoshells conjugated to ephrinA I, a ligand for EphA2 receptor that is overexpressed on PC-3 cells. We demonstrate selective photo-thermal destruction of these cells upon application of the NIR laser.

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Immunofluorescence staining of human prostate cancer cells. A) A model prostate cancer cell line, PC-3, stained with anti-EphA2 antibody, shows abundant expression level of EphA2 receptor C) whereas there is no detectable level in HDF cells. B), D) PC-3 and HDF cells incubated with only secondary antibody conjugated with Alex fluor 488 had minimal fluorescence background. DAPI is shown in blue.
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f1-ijn-3-351: Immunofluorescence staining of human prostate cancer cells. A) A model prostate cancer cell line, PC-3, stained with anti-EphA2 antibody, shows abundant expression level of EphA2 receptor C) whereas there is no detectable level in HDF cells. B), D) PC-3 and HDF cells incubated with only secondary antibody conjugated with Alex fluor 488 had minimal fluorescence background. DAPI is shown in blue.

Mentions: EphA2 are known to be overexpressed in prostate, melanoma, breast, and lung cancers (Walker-Daniels et al 1999; Zelinski et al 2001; Hess et al 2001; Brantley et al 2002; Kinch et al 2003; Ireton and Chen 2005). In order to confirm high expression levels of EphA2 in prostate cell lines, we performed immunofluorescence staining of EphA2 on PC-3 cells. As shown in Figure 1A and 1C, PC-3 cells incubated with antibody against the EphA2 receptor showed strong fluorescence (green) whereas HDF had minimal fluorescence signal. There was minimal background signal as shown with PC-3 cells and HDF incubated only with the secondary antibody conjugated with fluorophores (Figure 1B and 1D).


EphrinA I-targeted nanoshells for photothermal ablation of prostate cancer cells.

Gobin AM, Moon JJ, West JL - Int J Nanomedicine (2008)

Immunofluorescence staining of human prostate cancer cells. A) A model prostate cancer cell line, PC-3, stained with anti-EphA2 antibody, shows abundant expression level of EphA2 receptor C) whereas there is no detectable level in HDF cells. B), D) PC-3 and HDF cells incubated with only secondary antibody conjugated with Alex fluor 488 had minimal fluorescence background. DAPI is shown in blue.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2626934&req=5

f1-ijn-3-351: Immunofluorescence staining of human prostate cancer cells. A) A model prostate cancer cell line, PC-3, stained with anti-EphA2 antibody, shows abundant expression level of EphA2 receptor C) whereas there is no detectable level in HDF cells. B), D) PC-3 and HDF cells incubated with only secondary antibody conjugated with Alex fluor 488 had minimal fluorescence background. DAPI is shown in blue.
Mentions: EphA2 are known to be overexpressed in prostate, melanoma, breast, and lung cancers (Walker-Daniels et al 1999; Zelinski et al 2001; Hess et al 2001; Brantley et al 2002; Kinch et al 2003; Ireton and Chen 2005). In order to confirm high expression levels of EphA2 in prostate cell lines, we performed immunofluorescence staining of EphA2 on PC-3 cells. As shown in Figure 1A and 1C, PC-3 cells incubated with antibody against the EphA2 receptor showed strong fluorescence (green) whereas HDF had minimal fluorescence signal. There was minimal background signal as shown with PC-3 cells and HDF incubated only with the secondary antibody conjugated with fluorophores (Figure 1B and 1D).

Bottom Line: In this work, we sought to improve their specificity by targeting them to prostate tumor cells.We report selective targeting of PC-3 cells with nanoshells conjugated to ephrinA I, a ligand for EphA2 receptor that is overexpressed on PC-3 cells.We demonstrate selective photo-thermal destruction of these cells upon application of the NIR laser.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, TX 77251-1892, USA.

ABSTRACT
Gold-coated silica nanoshells are a class of nanoparticles that can be designed to possess strong absorption of light in the near infrared (NIR) wavelength region. When injected intravenously, these nanoshells have been shown to accumulate in tumors and subsequently mediate photothermal treatment, leading to tumor regression. In this work, we sought to improve their specificity by targeting them to prostate tumor cells. We report selective targeting of PC-3 cells with nanoshells conjugated to ephrinA I, a ligand for EphA2 receptor that is overexpressed on PC-3 cells. We demonstrate selective photo-thermal destruction of these cells upon application of the NIR laser.

Show MeSH
Related in: MedlinePlus