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Enhanced osteoblast adhesion on nanostructured selenium compacts for anti-cancer orthopedic applications.

Tran P, Webster TJ - Int J Nanomedicine (2008)

Bottom Line: Through these etching techniques, results of this study showed that biologically inspired surface roughness values were created on selenium compacts to match that of natural bone roughness.Moreover, results showed that healthy bone cell adhesion increased with greater nanometer selenium roughness (more closely matching that of titanium).In this manner, this study suggests that nano-rough selenium should be further tested for orthopedic applications involving bone cancer treatment.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, Brown University, Providence, RI 02912, USA.

ABSTRACT
Metallic bone implants possess numerous problems limiting their long-term efficacy, such as poor prolonged osseointegration, stress shielding, and corrosion under in vivo environments. Such problems are compounded for bone cancer patients since numerous patients receive orthopedic implants after cancerous bone resection. Unfortunately, current orthopedic materials were not originally developed to simultaneously increase healthy bone growth (as in traditional orthopedic implant applications) while inhibiting cancerous bone growth. The long-term objective of the present research is to investigate the use of nano-rough selenium to prevent bone cancer from re-occurring while promoting healthy bone growth for this select group of cancer patients. Selenium is a well known anti-cancer chemical. However, what is not known is how healthy bone cells interact with selenium. To determine this, selenium, spherical or semispherical shots, were pressed into cylindrical compacts and these compacts were then etched using 1N NaOH to obtain various surface structures ranging from the micron, submicron to nano scales. Changes in surface chemistry were also analyzed. Through these etching techniques, results of this study showed that biologically inspired surface roughness values were created on selenium compacts to match that of natural bone roughness. Moreover, results showed that healthy bone cell adhesion increased with greater nanometer selenium roughness (more closely matching that of titanium). In this manner, this study suggests that nano-rough selenium should be further tested for orthopedic applications involving bone cancer treatment.

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Fluorescence microscopy images of osteoblasts on: (A) Untreated selenium compacts; (B) selenium compacts treated with 1N NaOH for 10 min; (C) selenium compacts treated with 1N NaOH for 30 min; and Titanium (Ti) substrates.Note: Time = 24 h.
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f3-ijn-3-391: Fluorescence microscopy images of osteoblasts on: (A) Untreated selenium compacts; (B) selenium compacts treated with 1N NaOH for 10 min; (C) selenium compacts treated with 1N NaOH for 30 min; and Titanium (Ti) substrates.Note: Time = 24 h.

Mentions: When these substrates were used as substrates for culturing osteoblasts, after 1 day, significantly increased cell densities were observed on the increasing nano-rough surfaces (ie, compacts treated with 1N NaOH for 10 and 30 min) (Figures 3, 4). Especially, the compacts treated with 1N NaOH for 30 min, which had the largest amount of nanometer surface features, had the highest osteoblast density. However, although closer, these selenium compacts (ie, compacts treated with 1N NaOH for 30 min) had osteoblast densities lower than that of titanium. Future studies will need to utilize greater times and/or concentrations of NaOH etching to determine if osteoblast adhesion can be matched between selenium and titanium. Moreover, future studies will need to examine primary osteoblast cells (since that was not accomplished here) as well as the multitude of cancerous cells that can cause bone cancer.


Enhanced osteoblast adhesion on nanostructured selenium compacts for anti-cancer orthopedic applications.

Tran P, Webster TJ - Int J Nanomedicine (2008)

Fluorescence microscopy images of osteoblasts on: (A) Untreated selenium compacts; (B) selenium compacts treated with 1N NaOH for 10 min; (C) selenium compacts treated with 1N NaOH for 30 min; and Titanium (Ti) substrates.Note: Time = 24 h.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2626931&req=5

f3-ijn-3-391: Fluorescence microscopy images of osteoblasts on: (A) Untreated selenium compacts; (B) selenium compacts treated with 1N NaOH for 10 min; (C) selenium compacts treated with 1N NaOH for 30 min; and Titanium (Ti) substrates.Note: Time = 24 h.
Mentions: When these substrates were used as substrates for culturing osteoblasts, after 1 day, significantly increased cell densities were observed on the increasing nano-rough surfaces (ie, compacts treated with 1N NaOH for 10 and 30 min) (Figures 3, 4). Especially, the compacts treated with 1N NaOH for 30 min, which had the largest amount of nanometer surface features, had the highest osteoblast density. However, although closer, these selenium compacts (ie, compacts treated with 1N NaOH for 30 min) had osteoblast densities lower than that of titanium. Future studies will need to utilize greater times and/or concentrations of NaOH etching to determine if osteoblast adhesion can be matched between selenium and titanium. Moreover, future studies will need to examine primary osteoblast cells (since that was not accomplished here) as well as the multitude of cancerous cells that can cause bone cancer.

Bottom Line: Through these etching techniques, results of this study showed that biologically inspired surface roughness values were created on selenium compacts to match that of natural bone roughness.Moreover, results showed that healthy bone cell adhesion increased with greater nanometer selenium roughness (more closely matching that of titanium).In this manner, this study suggests that nano-rough selenium should be further tested for orthopedic applications involving bone cancer treatment.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, Brown University, Providence, RI 02912, USA.

ABSTRACT
Metallic bone implants possess numerous problems limiting their long-term efficacy, such as poor prolonged osseointegration, stress shielding, and corrosion under in vivo environments. Such problems are compounded for bone cancer patients since numerous patients receive orthopedic implants after cancerous bone resection. Unfortunately, current orthopedic materials were not originally developed to simultaneously increase healthy bone growth (as in traditional orthopedic implant applications) while inhibiting cancerous bone growth. The long-term objective of the present research is to investigate the use of nano-rough selenium to prevent bone cancer from re-occurring while promoting healthy bone growth for this select group of cancer patients. Selenium is a well known anti-cancer chemical. However, what is not known is how healthy bone cells interact with selenium. To determine this, selenium, spherical or semispherical shots, were pressed into cylindrical compacts and these compacts were then etched using 1N NaOH to obtain various surface structures ranging from the micron, submicron to nano scales. Changes in surface chemistry were also analyzed. Through these etching techniques, results of this study showed that biologically inspired surface roughness values were created on selenium compacts to match that of natural bone roughness. Moreover, results showed that healthy bone cell adhesion increased with greater nanometer selenium roughness (more closely matching that of titanium). In this manner, this study suggests that nano-rough selenium should be further tested for orthopedic applications involving bone cancer treatment.

Show MeSH
Related in: MedlinePlus