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Biomimetic helical rosette nanotubes and nanocrystalline hydroxyapatite coatings on titanium for improving orthopedic implants.

Zhang L, Chen Y, Rodriguez J, Fenniri H, Webster TJ - Int J Nanomedicine (2008)

Bottom Line: Some of the nanocrystalline HA formed dense coatings with HRNs on titanium.More importantly, results demonstrated enhanced osteoblast adhesion on the HRN/nanocrystalline HA-coated titanium compared with conventional uncoated titanium.Among all the HRN/nanocrystalline HA coatings tested, osteoblast adhesion was the greatest when HA nanometer particle size was the smallest.

View Article: PubMed Central - PubMed

Affiliation: Division of Engineering, Brown University, Providence, RI 02912, USA.

ABSTRACT
Natural bone consists of hard nanostructured hydroxyapatite (HA) in a nanostructured protein-based soft hydrogel template (ie, mostly collagen). For this reason, nanostructured HA has been an intriguing coating material on traditionally used titanium for improving orthopedic applications. In addition, helical rosette nanotubes (HRNs), newly developed materials which form through the self-assembly process of DNA base pair building blocks in body solutions, are soft nanotubes with a helical architecture that mimics natural collagen. Thus, the objective of this in vitro study was for the first time to combine the promising attributes of HRNs and nanocrystalline HA on titanium and assess osteoblast (bone-forming cell) functions. Different sizes of nanocrystalline HA were synthesized in this study through a wet chemical precipitation process following either hydrothermal treatment or sintering. Transmission electron microscopy images showed that HRNs aligned with nanocrystalline HA, which indicates a high affinity between both components. Some of the nanocrystalline HA formed dense coatings with HRNs on titanium. More importantly, results demonstrated enhanced osteoblast adhesion on the HRN/nanocrystalline HA-coated titanium compared with conventional uncoated titanium. Among all the HRN/nanocrystalline HA coatings tested, osteoblast adhesion was the greatest when HA nanometer particle size was the smallest. In this manner, this study demonstrated for the first time that biomimetic HRN/nanocrystalline HA coatings on titanium were cytocompatible for osteoblasts and, thus, should be further studied for improving orthopedic implants.

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The comparison of average grain sizes of nanocrystalline HA under different synthesis treatments. Data are mean ± SEM; n = 50. *p < 0.0001 when compared with small grain size nanocrystalline HA (prepared by hydrothermal treatment), and **p < 0.0001 when compared with large grain size nanocrystalline HA (prepared by sintering at 700 °C).
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f3-ijn-3-323: The comparison of average grain sizes of nanocrystalline HA under different synthesis treatments. Data are mean ± SEM; n = 50. *p < 0.0001 when compared with small grain size nanocrystalline HA (prepared by hydrothermal treatment), and **p < 0.0001 when compared with large grain size nanocrystalline HA (prepared by sintering at 700 °C).

Mentions: Furthermore, by measuring the sizes of nanocrystalline HA in TEM images, Figure 3 provided quantified results of the average HA grain sizes after hydrothermally treatment and sintering which demonstrated that hydrothermal treatment can yield much smaller crystallite sizes than other methods. Specifically, nanocrystalline HA prepared by the hydrothermal treatment had an average grain size of 82.84 nm (in length) while nanocrystalline HA particles prepared by sintering at either 550 °C or 700 °C had average grain sizes of 90.01nm and 129.81nm, respectively.


Biomimetic helical rosette nanotubes and nanocrystalline hydroxyapatite coatings on titanium for improving orthopedic implants.

Zhang L, Chen Y, Rodriguez J, Fenniri H, Webster TJ - Int J Nanomedicine (2008)

The comparison of average grain sizes of nanocrystalline HA under different synthesis treatments. Data are mean ± SEM; n = 50. *p < 0.0001 when compared with small grain size nanocrystalline HA (prepared by hydrothermal treatment), and **p < 0.0001 when compared with large grain size nanocrystalline HA (prepared by sintering at 700 °C).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2626930&req=5

f3-ijn-3-323: The comparison of average grain sizes of nanocrystalline HA under different synthesis treatments. Data are mean ± SEM; n = 50. *p < 0.0001 when compared with small grain size nanocrystalline HA (prepared by hydrothermal treatment), and **p < 0.0001 when compared with large grain size nanocrystalline HA (prepared by sintering at 700 °C).
Mentions: Furthermore, by measuring the sizes of nanocrystalline HA in TEM images, Figure 3 provided quantified results of the average HA grain sizes after hydrothermally treatment and sintering which demonstrated that hydrothermal treatment can yield much smaller crystallite sizes than other methods. Specifically, nanocrystalline HA prepared by the hydrothermal treatment had an average grain size of 82.84 nm (in length) while nanocrystalline HA particles prepared by sintering at either 550 °C or 700 °C had average grain sizes of 90.01nm and 129.81nm, respectively.

Bottom Line: Some of the nanocrystalline HA formed dense coatings with HRNs on titanium.More importantly, results demonstrated enhanced osteoblast adhesion on the HRN/nanocrystalline HA-coated titanium compared with conventional uncoated titanium.Among all the HRN/nanocrystalline HA coatings tested, osteoblast adhesion was the greatest when HA nanometer particle size was the smallest.

View Article: PubMed Central - PubMed

Affiliation: Division of Engineering, Brown University, Providence, RI 02912, USA.

ABSTRACT
Natural bone consists of hard nanostructured hydroxyapatite (HA) in a nanostructured protein-based soft hydrogel template (ie, mostly collagen). For this reason, nanostructured HA has been an intriguing coating material on traditionally used titanium for improving orthopedic applications. In addition, helical rosette nanotubes (HRNs), newly developed materials which form through the self-assembly process of DNA base pair building blocks in body solutions, are soft nanotubes with a helical architecture that mimics natural collagen. Thus, the objective of this in vitro study was for the first time to combine the promising attributes of HRNs and nanocrystalline HA on titanium and assess osteoblast (bone-forming cell) functions. Different sizes of nanocrystalline HA were synthesized in this study through a wet chemical precipitation process following either hydrothermal treatment or sintering. Transmission electron microscopy images showed that HRNs aligned with nanocrystalline HA, which indicates a high affinity between both components. Some of the nanocrystalline HA formed dense coatings with HRNs on titanium. More importantly, results demonstrated enhanced osteoblast adhesion on the HRN/nanocrystalline HA-coated titanium compared with conventional uncoated titanium. Among all the HRN/nanocrystalline HA coatings tested, osteoblast adhesion was the greatest when HA nanometer particle size was the smallest. In this manner, this study demonstrated for the first time that biomimetic HRN/nanocrystalline HA coatings on titanium were cytocompatible for osteoblasts and, thus, should be further studied for improving orthopedic implants.

Show MeSH
Related in: MedlinePlus