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Labeling efficacy of superparamagnetic iron oxide nanoparticles to human neural stem cells: comparison of ferumoxides, monocrystalline iron oxide, cross-linked iron oxide (CLIO)-NH2 and tat-CLIO.

Song M, Moon WK, Kim Y, Lim D, Song IC, Yoon BW - Korean J Radiol (2007 Sep-Oct)

Bottom Line: The hNSCs labeled with tat-CLIO showed the longest retention, up to 72 hr, and they contained 2.15+/-0.3 pg iron/cell, which are 59 fold, 430 fold and six fold more incorporated iron than that of the hNSCs labeled with ferumoxides, MION or CLIO-NH(2), respectively.However, when PLL was added, the incorporation of ferumoxides, MION or CLIO-NH(2) into the hNSCs was comparable to that of tat-CLIO.For MR imaging, hNSCs can be efficiently labeled with tat-CLIO alone or with a combination of ferumoxides, MION, CLIO-NH(2) and the transfection agent PLL.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Clinical Research Institute, Seoul National University Hospital, Seoul National University, Seoul, Korea.

ABSTRACT

Objective: We wanted to compare the human neural stem cell (hNSC) labeling efficacy of different superparamagnetic iron oxide nanoparticles (SPIONs), namely, ferumoxides, monocrystalline iron oxide (MION), cross-linked iron oxide (CLIO)-NH(2) and tat-CLIO.

Materials and methods: The hNSCs (5 x 10(5) HB1F3 cells/ml) were incubated for 24 hr in cell culture media that contained 25 microg/ml of ferumoxides, MION or CLIO-NH(2), and with or without poly-L-lysine (PLL) and tat-CLIO. The cellular iron uptake was analyzed qualitatively with using a light microscope and this was quantified via atomic absorption spectrophotometry. The visibility of the labeled cells was assessed with MR imaging.

Results: The incorporation of SPIONs into the hNSCs did not affect the cellular proliferations and viabilities. The hNSCs labeled with tat-CLIO showed the longest retention, up to 72 hr, and they contained 2.15+/-0.3 pg iron/cell, which are 59 fold, 430 fold and six fold more incorporated iron than that of the hNSCs labeled with ferumoxides, MION or CLIO-NH(2), respectively. However, when PLL was added, the incorporation of ferumoxides, MION or CLIO-NH(2) into the hNSCs was comparable to that of tat-CLIO.

Conclusion: For MR imaging, hNSCs can be efficiently labeled with tat-CLIO alone or with a combination of ferumoxides, MION, CLIO-NH(2) and the transfection agent PLL.

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Related in: MedlinePlus

Photomicrographs of the hNSCs treated for 24 hr with ferumoxides (A), MION-47 (B), CLIO-NH2 (C) or tat-CLIO (D) at 25 µg/ml. The intracellular uptake of iron oxide nanoparticles (arrows) is seen in cells exposed to ferumoxides (A), CLIO-NH2 (C) or tat-CLIO (D). However, no intracellular uptake of iron oxide was found for the cells incubated with MION-47 (B). (Prussian blue stain, objective magnification: × 40)
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Figure 1: Photomicrographs of the hNSCs treated for 24 hr with ferumoxides (A), MION-47 (B), CLIO-NH2 (C) or tat-CLIO (D) at 25 µg/ml. The intracellular uptake of iron oxide nanoparticles (arrows) is seen in cells exposed to ferumoxides (A), CLIO-NH2 (C) or tat-CLIO (D). However, no intracellular uptake of iron oxide was found for the cells incubated with MION-47 (B). (Prussian blue stain, objective magnification: × 40)

Mentions: The HB1F3 cells exposed to the ferumoxides, MION-47, CLIO-NH2 or tat-CLIO showed intracellular uptake of the iron oxide (Fig. 1). However, no intracellular uptake of the iron oxide was detected in cells incubated with MION-47.


Labeling efficacy of superparamagnetic iron oxide nanoparticles to human neural stem cells: comparison of ferumoxides, monocrystalline iron oxide, cross-linked iron oxide (CLIO)-NH2 and tat-CLIO.

Song M, Moon WK, Kim Y, Lim D, Song IC, Yoon BW - Korean J Radiol (2007 Sep-Oct)

Photomicrographs of the hNSCs treated for 24 hr with ferumoxides (A), MION-47 (B), CLIO-NH2 (C) or tat-CLIO (D) at 25 µg/ml. The intracellular uptake of iron oxide nanoparticles (arrows) is seen in cells exposed to ferumoxides (A), CLIO-NH2 (C) or tat-CLIO (D). However, no intracellular uptake of iron oxide was found for the cells incubated with MION-47 (B). (Prussian blue stain, objective magnification: × 40)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2626816&req=5

Figure 1: Photomicrographs of the hNSCs treated for 24 hr with ferumoxides (A), MION-47 (B), CLIO-NH2 (C) or tat-CLIO (D) at 25 µg/ml. The intracellular uptake of iron oxide nanoparticles (arrows) is seen in cells exposed to ferumoxides (A), CLIO-NH2 (C) or tat-CLIO (D). However, no intracellular uptake of iron oxide was found for the cells incubated with MION-47 (B). (Prussian blue stain, objective magnification: × 40)
Mentions: The HB1F3 cells exposed to the ferumoxides, MION-47, CLIO-NH2 or tat-CLIO showed intracellular uptake of the iron oxide (Fig. 1). However, no intracellular uptake of the iron oxide was detected in cells incubated with MION-47.

Bottom Line: The hNSCs labeled with tat-CLIO showed the longest retention, up to 72 hr, and they contained 2.15+/-0.3 pg iron/cell, which are 59 fold, 430 fold and six fold more incorporated iron than that of the hNSCs labeled with ferumoxides, MION or CLIO-NH(2), respectively.However, when PLL was added, the incorporation of ferumoxides, MION or CLIO-NH(2) into the hNSCs was comparable to that of tat-CLIO.For MR imaging, hNSCs can be efficiently labeled with tat-CLIO alone or with a combination of ferumoxides, MION, CLIO-NH(2) and the transfection agent PLL.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Clinical Research Institute, Seoul National University Hospital, Seoul National University, Seoul, Korea.

ABSTRACT

Objective: We wanted to compare the human neural stem cell (hNSC) labeling efficacy of different superparamagnetic iron oxide nanoparticles (SPIONs), namely, ferumoxides, monocrystalline iron oxide (MION), cross-linked iron oxide (CLIO)-NH(2) and tat-CLIO.

Materials and methods: The hNSCs (5 x 10(5) HB1F3 cells/ml) were incubated for 24 hr in cell culture media that contained 25 microg/ml of ferumoxides, MION or CLIO-NH(2), and with or without poly-L-lysine (PLL) and tat-CLIO. The cellular iron uptake was analyzed qualitatively with using a light microscope and this was quantified via atomic absorption spectrophotometry. The visibility of the labeled cells was assessed with MR imaging.

Results: The incorporation of SPIONs into the hNSCs did not affect the cellular proliferations and viabilities. The hNSCs labeled with tat-CLIO showed the longest retention, up to 72 hr, and they contained 2.15+/-0.3 pg iron/cell, which are 59 fold, 430 fold and six fold more incorporated iron than that of the hNSCs labeled with ferumoxides, MION or CLIO-NH(2), respectively. However, when PLL was added, the incorporation of ferumoxides, MION or CLIO-NH(2) into the hNSCs was comparable to that of tat-CLIO.

Conclusion: For MR imaging, hNSCs can be efficiently labeled with tat-CLIO alone or with a combination of ferumoxides, MION, CLIO-NH(2) and the transfection agent PLL.

Show MeSH
Related in: MedlinePlus