Limits...
The 5-choice continuous performance test: evidence for a translational test of vigilance for mice.

Young JW, Light GA, Marston HM, Sharp R, Geyer MA - PLoS ONE (2009)

Bottom Line: Although vigilance is assessed frequently using the continuous performance test (CPT) in humans, few tests specifically assess vigilance in rodents.Finally, we observed increased RTs with increased attentional load, such that 1CRT task<5CSR task<5C-CPT, consistent with human performance in simple RT, choice RT, and CPT tasks.Thus we have demonstrated construct validity for the 5C-CPT as a measure of vigilance that is analogous to human CPT studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychiatry, University of California San Diego, La Jolla, California, United States of America. jaredyoung@ucsd.edu

ABSTRACT

Background: Attentional dysfunction is related to functional disability in patients with neuropsychiatric disorders such as schizophrenia, bipolar disorder, and Alzheimer's disease. Indeed, sustained attention/vigilance is among the leading targets for new medications designed to improve cognition in schizophrenia. Although vigilance is assessed frequently using the continuous performance test (CPT) in humans, few tests specifically assess vigilance in rodents.

Methods: We describe the 5-choice CPT (5C-CPT), an elaboration of the 5-choice serial reaction (5CSR) task that includes non-signal trials, thus mimicking task parameters of human CPTs that use signal and non-signal events to assess vigilance. The performances of C57BL/6J and DBA/2J mice were assessed in the 5C-CPT to determine whether this task could differentiate between strains. C57BL/6J mice were also trained in the 5CSR task and a simple reaction-time (RT) task involving only one choice (1CRT task). We hypothesized that: 1) C57BL/6J performance would be superior to DBA/2J mice in the 5C-CPT as measured by the sensitivity index measure from signal detection theory; 2) a vigilance decrement would be observed in both strains; and 3) RTs would increase across tasks with increased attentional load (1CRT task<5CSR task<5C-CPT).

Conclusions: C57BL/6J mice exhibited superior SI levels compared to DBA/2J mice, but with no difference in accuracy. A vigilance decrement was observed in both strains, which was more pronounced in DBA/2J mice and unaffected by response bias. Finally, we observed increased RTs with increased attentional load, such that 1CRT task<5CSR task<5C-CPT, consistent with human performance in simple RT, choice RT, and CPT tasks. Thus we have demonstrated construct validity for the 5C-CPT as a measure of vigilance that is analogous to human CPT studies.

Show MeSH

Related in: MedlinePlus

Comparison of percentage non-responding in no-go vs. go trials in the 5C-CPT.Given the similarity in response type for a correct rejection in a no-go trial to a miss in a go trial (both are represented by a lack of response), it is important to establish that the performance of the mice is dependent upon trial type. C57BL/6J and DBA/2J mice exhibited comparable levels of %Correct Rejection (%Corr Rej) during no-go trials in the extended session 5C-CPT. During go trials however, C57BL/6J mice exhibit significantly lower levels of %Omissions compared to DBA/2J mice. Importantly, both groups exhibited significantly different levels of non-response to each trial type, suggesting that they performed differently during go trials compared to no-go trials, and hence they their response was dependent upon trial type. Data presented as mean+s.e.m., and * denotes p<0.05.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2626630&req=5

pone-0004227-g006: Comparison of percentage non-responding in no-go vs. go trials in the 5C-CPT.Given the similarity in response type for a correct rejection in a no-go trial to a miss in a go trial (both are represented by a lack of response), it is important to establish that the performance of the mice is dependent upon trial type. C57BL/6J and DBA/2J mice exhibited comparable levels of %Correct Rejection (%Corr Rej) during no-go trials in the extended session 5C-CPT. During go trials however, C57BL/6J mice exhibit significantly lower levels of %Omissions compared to DBA/2J mice. Importantly, both groups exhibited significantly different levels of non-response to each trial type, suggesting that they performed differently during go trials compared to no-go trials, and hence they their response was dependent upon trial type. Data presented as mean+s.e.m., and * denotes p<0.05.

Mentions: Given that an omission error in a go trial results from the same behavior as a correct rejection in a no-go trial (i.e. lack of response to a cue stimulus), %Correct Rejections from no-go trials were compared to %Omissions from go trials (from the extended session) to assess whether mice treated the two trial types the same. Significant differences between %Correct Rejection and %Omissions were observed for C57BL/6J (F(1,17) = 66.2, p<0.0001; Fig 6) and DBA/2J mice (F(1,12) = 7.7, p<0.05; Fig 6). The effect sizes for C57BL/6J and DBA/2J mice were 0.796 and 0.391 respectively, suggesting a greater differentiation between scores for C57BL/6J compared to DBA/2J mice.


The 5-choice continuous performance test: evidence for a translational test of vigilance for mice.

Young JW, Light GA, Marston HM, Sharp R, Geyer MA - PLoS ONE (2009)

Comparison of percentage non-responding in no-go vs. go trials in the 5C-CPT.Given the similarity in response type for a correct rejection in a no-go trial to a miss in a go trial (both are represented by a lack of response), it is important to establish that the performance of the mice is dependent upon trial type. C57BL/6J and DBA/2J mice exhibited comparable levels of %Correct Rejection (%Corr Rej) during no-go trials in the extended session 5C-CPT. During go trials however, C57BL/6J mice exhibit significantly lower levels of %Omissions compared to DBA/2J mice. Importantly, both groups exhibited significantly different levels of non-response to each trial type, suggesting that they performed differently during go trials compared to no-go trials, and hence they their response was dependent upon trial type. Data presented as mean+s.e.m., and * denotes p<0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2626630&req=5

pone-0004227-g006: Comparison of percentage non-responding in no-go vs. go trials in the 5C-CPT.Given the similarity in response type for a correct rejection in a no-go trial to a miss in a go trial (both are represented by a lack of response), it is important to establish that the performance of the mice is dependent upon trial type. C57BL/6J and DBA/2J mice exhibited comparable levels of %Correct Rejection (%Corr Rej) during no-go trials in the extended session 5C-CPT. During go trials however, C57BL/6J mice exhibit significantly lower levels of %Omissions compared to DBA/2J mice. Importantly, both groups exhibited significantly different levels of non-response to each trial type, suggesting that they performed differently during go trials compared to no-go trials, and hence they their response was dependent upon trial type. Data presented as mean+s.e.m., and * denotes p<0.05.
Mentions: Given that an omission error in a go trial results from the same behavior as a correct rejection in a no-go trial (i.e. lack of response to a cue stimulus), %Correct Rejections from no-go trials were compared to %Omissions from go trials (from the extended session) to assess whether mice treated the two trial types the same. Significant differences between %Correct Rejection and %Omissions were observed for C57BL/6J (F(1,17) = 66.2, p<0.0001; Fig 6) and DBA/2J mice (F(1,12) = 7.7, p<0.05; Fig 6). The effect sizes for C57BL/6J and DBA/2J mice were 0.796 and 0.391 respectively, suggesting a greater differentiation between scores for C57BL/6J compared to DBA/2J mice.

Bottom Line: Although vigilance is assessed frequently using the continuous performance test (CPT) in humans, few tests specifically assess vigilance in rodents.Finally, we observed increased RTs with increased attentional load, such that 1CRT task<5CSR task<5C-CPT, consistent with human performance in simple RT, choice RT, and CPT tasks.Thus we have demonstrated construct validity for the 5C-CPT as a measure of vigilance that is analogous to human CPT studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychiatry, University of California San Diego, La Jolla, California, United States of America. jaredyoung@ucsd.edu

ABSTRACT

Background: Attentional dysfunction is related to functional disability in patients with neuropsychiatric disorders such as schizophrenia, bipolar disorder, and Alzheimer's disease. Indeed, sustained attention/vigilance is among the leading targets for new medications designed to improve cognition in schizophrenia. Although vigilance is assessed frequently using the continuous performance test (CPT) in humans, few tests specifically assess vigilance in rodents.

Methods: We describe the 5-choice CPT (5C-CPT), an elaboration of the 5-choice serial reaction (5CSR) task that includes non-signal trials, thus mimicking task parameters of human CPTs that use signal and non-signal events to assess vigilance. The performances of C57BL/6J and DBA/2J mice were assessed in the 5C-CPT to determine whether this task could differentiate between strains. C57BL/6J mice were also trained in the 5CSR task and a simple reaction-time (RT) task involving only one choice (1CRT task). We hypothesized that: 1) C57BL/6J performance would be superior to DBA/2J mice in the 5C-CPT as measured by the sensitivity index measure from signal detection theory; 2) a vigilance decrement would be observed in both strains; and 3) RTs would increase across tasks with increased attentional load (1CRT task<5CSR task<5C-CPT).

Conclusions: C57BL/6J mice exhibited superior SI levels compared to DBA/2J mice, but with no difference in accuracy. A vigilance decrement was observed in both strains, which was more pronounced in DBA/2J mice and unaffected by response bias. Finally, we observed increased RTs with increased attentional load, such that 1CRT task<5CSR task<5C-CPT, consistent with human performance in simple RT, choice RT, and CPT tasks. Thus we have demonstrated construct validity for the 5C-CPT as a measure of vigilance that is analogous to human CPT studies.

Show MeSH
Related in: MedlinePlus