Limits...
Gene Chip for Viral Discovery

View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Monkey pox... SARS... Researchers led by Joseph DeRisi of the University of California at San Francisco have combined genome databases of sequenced viruses with DNA microarray technology to create such tools... The viral gene chip they created can rapidly identify known viruses and classify new ones based on their genetic makeup... This was validated in March when the viral chip contributed to the identification of the cause for severe acute respiratory syndrome (SARS) as a novel coronavirus... In the article published in this issue, the researchers describe the chip (or microarray), how it was used in the classification of the SARS virus, and how it provides direct access to viral genomic sequence... Microarray technology works by taking advantage of the structural properties of DNA... DNA molecules normally exist as double helices, two complementary strands of nucleotides wrapped around each other... The microarray consists of a large number of single DNA strands attached to a solid base... These probes (which in case of the viral chip represent sequences from all fully sequenced reference viruses) can be used to interrogate unknown sequences: if a solution containing such sequences is passed over the chip, similar sequences will “hybridize,” or bond in a signature double helix... To quickly obtain more information on a novel virus, it is then possible to “syphon off” those viral sequences that stuck to their respective counterparts on the chip and to use the material to determine part of the genomic sequence.

No MeSH data available.


Extraction, amplification, and decoding of viral sequences
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC261871&req=5

pbio.0000003-g001: Extraction, amplification, and decoding of viral sequences


Gene Chip for Viral Discovery
Extraction, amplification, and decoding of viral sequences
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC261871&req=5

pbio.0000003-g001: Extraction, amplification, and decoding of viral sequences

View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Monkey pox... SARS... Researchers led by Joseph DeRisi of the University of California at San Francisco have combined genome databases of sequenced viruses with DNA microarray technology to create such tools... The viral gene chip they created can rapidly identify known viruses and classify new ones based on their genetic makeup... This was validated in March when the viral chip contributed to the identification of the cause for severe acute respiratory syndrome (SARS) as a novel coronavirus... In the article published in this issue, the researchers describe the chip (or microarray), how it was used in the classification of the SARS virus, and how it provides direct access to viral genomic sequence... Microarray technology works by taking advantage of the structural properties of DNA... DNA molecules normally exist as double helices, two complementary strands of nucleotides wrapped around each other... The microarray consists of a large number of single DNA strands attached to a solid base... These probes (which in case of the viral chip represent sequences from all fully sequenced reference viruses) can be used to interrogate unknown sequences: if a solution containing such sequences is passed over the chip, similar sequences will “hybridize,” or bond in a signature double helix... To quickly obtain more information on a novel virus, it is then possible to “syphon off” those viral sequences that stuck to their respective counterparts on the chip and to use the material to determine part of the genomic sequence.

No MeSH data available.