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Evaluation of cariogenic bacteria.

Nishikawara F, Nomura Y, Imai S, Senda A, Hanada N - Eur J Dent (2007)

Bottom Line: The ratios of MS in detected bacteria were compared by ELISA.The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRT(TM), (P< .01) and Dentocult SM(TM) (P<.05).The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium.

View Article: PubMed Central - PubMed

Affiliation: Department of Preventive Dentistry and Public Health, School of Dental Medicine, Tsurumi University,Japan.

ABSTRACT

Objectives: The evaluation of Mutans streptococci (MS) is one of the index for caries risk. Dentocult(TM) and CRT(TM) are commercial kits to detect and evaluate MS, conveniently. However, the evaluation of MS has also been carried out simply using an instruction manual. But the instruction manual is not easy to use for evaluation of MS. The aim of this study was to examine the utility of modified Mitis-Salivalius Bacitracin (MSB) agar medium compared with MSB agar medium and commercial kits, and to establish a convenient kit (mMSB-kit) using modified MSB agar.

Methods: The MS in stimulated saliva from 27 subjects were detected by MSB, modified MSB agar medium and commercial kits. Laboratory and clinically isolated strains of MS were similarly evaluated. The ratios of MS in detected bacteria were compared by ELISA.

Results: The scores using an mMSB-kit on the basis of modified MSB agar medium were tabulated. Saliva samples showed different levels of MS between culture methods and the commercial kit. Some samples which were full of MS were not detected by the commercial kit. The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRT(TM), (P< .01) and Dentocult SM(TM) (P<.05).

Conclusion: The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium. The mMSB-kit can be used simply, and can be an important contributor for the evaluation of MS as a caries risk factor.

No MeSH data available.


Related in: MedlinePlus

Ratios of S. mutans and S. sobrinus calculated by different methods.Differences in the ratio of S. mutans and S. sobrinus were different for modified MSB agar medium and mMSB-kit compared to the Dentocult SMTM (P<.05: Mann-Whitney test), CRTTM and MSB agar medium (P<.01: Mann-Whitney test). However, differences in the S. mutans and S. sobrinus ratios were not significant between the Dentocult SMTM, CRTTM and MSB agar medium.
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f3-0010031: Ratios of S. mutans and S. sobrinus calculated by different methods.Differences in the ratio of S. mutans and S. sobrinus were different for modified MSB agar medium and mMSB-kit compared to the Dentocult SMTM (P<.05: Mann-Whitney test), CRTTM and MSB agar medium (P<.01: Mann-Whitney test). However, differences in the S. mutans and S. sobrinus ratios were not significant between the Dentocult SMTM, CRTTM and MSB agar medium.

Mentions: Figure 3 summarizes the ratio of cariogenic bacteria to total streptococci when antibodies for S. mutans and S. sobrinus and identification with ELISA were used. Levels of cariogenic bacteria in each subject analyzed with MSB agar medium, modified MSB agar medium, commercialized kits and mMSB-kit for detection of S. mutans and S. sobrinus. Modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium, CRTTM (P<.01) and Dentocult SMTM (P<.05). There was no significant difference between results for modified MSB agar medium and mMSB-kit.


Evaluation of cariogenic bacteria.

Nishikawara F, Nomura Y, Imai S, Senda A, Hanada N - Eur J Dent (2007)

Ratios of S. mutans and S. sobrinus calculated by different methods.Differences in the ratio of S. mutans and S. sobrinus were different for modified MSB agar medium and mMSB-kit compared to the Dentocult SMTM (P<.05: Mann-Whitney test), CRTTM and MSB agar medium (P<.01: Mann-Whitney test). However, differences in the S. mutans and S. sobrinus ratios were not significant between the Dentocult SMTM, CRTTM and MSB agar medium.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2612953&req=5

f3-0010031: Ratios of S. mutans and S. sobrinus calculated by different methods.Differences in the ratio of S. mutans and S. sobrinus were different for modified MSB agar medium and mMSB-kit compared to the Dentocult SMTM (P<.05: Mann-Whitney test), CRTTM and MSB agar medium (P<.01: Mann-Whitney test). However, differences in the S. mutans and S. sobrinus ratios were not significant between the Dentocult SMTM, CRTTM and MSB agar medium.
Mentions: Figure 3 summarizes the ratio of cariogenic bacteria to total streptococci when antibodies for S. mutans and S. sobrinus and identification with ELISA were used. Levels of cariogenic bacteria in each subject analyzed with MSB agar medium, modified MSB agar medium, commercialized kits and mMSB-kit for detection of S. mutans and S. sobrinus. Modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium, CRTTM (P<.01) and Dentocult SMTM (P<.05). There was no significant difference between results for modified MSB agar medium and mMSB-kit.

Bottom Line: The ratios of MS in detected bacteria were compared by ELISA.The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRT(TM), (P< .01) and Dentocult SM(TM) (P<.05).The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium.

View Article: PubMed Central - PubMed

Affiliation: Department of Preventive Dentistry and Public Health, School of Dental Medicine, Tsurumi University,Japan.

ABSTRACT

Objectives: The evaluation of Mutans streptococci (MS) is one of the index for caries risk. Dentocult(TM) and CRT(TM) are commercial kits to detect and evaluate MS, conveniently. However, the evaluation of MS has also been carried out simply using an instruction manual. But the instruction manual is not easy to use for evaluation of MS. The aim of this study was to examine the utility of modified Mitis-Salivalius Bacitracin (MSB) agar medium compared with MSB agar medium and commercial kits, and to establish a convenient kit (mMSB-kit) using modified MSB agar.

Methods: The MS in stimulated saliva from 27 subjects were detected by MSB, modified MSB agar medium and commercial kits. Laboratory and clinically isolated strains of MS were similarly evaluated. The ratios of MS in detected bacteria were compared by ELISA.

Results: The scores using an mMSB-kit on the basis of modified MSB agar medium were tabulated. Saliva samples showed different levels of MS between culture methods and the commercial kit. Some samples which were full of MS were not detected by the commercial kit. The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRT(TM), (P< .01) and Dentocult SM(TM) (P<.05).

Conclusion: The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium. The mMSB-kit can be used simply, and can be an important contributor for the evaluation of MS as a caries risk factor.

No MeSH data available.


Related in: MedlinePlus