Limits...
Evaluation of cariogenic bacteria.

Nishikawara F, Nomura Y, Imai S, Senda A, Hanada N - Eur J Dent (2007)

Bottom Line: The ratios of MS in detected bacteria were compared by ELISA.The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRT(TM), (P< .01) and Dentocult SM(TM) (P<.05).The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium.

View Article: PubMed Central - PubMed

Affiliation: Department of Preventive Dentistry and Public Health, School of Dental Medicine, Tsurumi University,Japan.

ABSTRACT

Objectives: The evaluation of Mutans streptococci (MS) is one of the index for caries risk. Dentocult(TM) and CRT(TM) are commercial kits to detect and evaluate MS, conveniently. However, the evaluation of MS has also been carried out simply using an instruction manual. But the instruction manual is not easy to use for evaluation of MS. The aim of this study was to examine the utility of modified Mitis-Salivalius Bacitracin (MSB) agar medium compared with MSB agar medium and commercial kits, and to establish a convenient kit (mMSB-kit) using modified MSB agar.

Methods: The MS in stimulated saliva from 27 subjects were detected by MSB, modified MSB agar medium and commercial kits. Laboratory and clinically isolated strains of MS were similarly evaluated. The ratios of MS in detected bacteria were compared by ELISA.

Results: The scores using an mMSB-kit on the basis of modified MSB agar medium were tabulated. Saliva samples showed different levels of MS between culture methods and the commercial kit. Some samples which were full of MS were not detected by the commercial kit. The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRT(TM), (P< .01) and Dentocult SM(TM) (P<.05).

Conclusion: The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium. The mMSB-kit can be used simply, and can be an important contributor for the evaluation of MS as a caries risk factor.

No MeSH data available.


Related in: MedlinePlus

Detection of MS was compared between MSB agar medium and Dentocult SMTM.The detection limits for MS on MSB agar plates were 0.Score for Dentocult SMTM and CRTTM are as follows, score 3: >6 log10CFU/ml, score 2: 6-5 log10CFU/ml, score 0-1: <5 log10CFU/ml.There was a positive association between MSB agar medium and Dentocult SMTM (P<.05, rs=0.53), whereas MSB agar medium and CRTTM showed no correlation (P<.05, rs=0.30) by Spearman.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2612953&req=5

f2a-0010031: Detection of MS was compared between MSB agar medium and Dentocult SMTM.The detection limits for MS on MSB agar plates were 0.Score for Dentocult SMTM and CRTTM are as follows, score 3: >6 log10CFU/ml, score 2: 6-5 log10CFU/ml, score 0-1: <5 log10CFU/ml.There was a positive association between MSB agar medium and Dentocult SMTM (P<.05, rs=0.53), whereas MSB agar medium and CRTTM showed no correlation (P<.05, rs=0.30) by Spearman.

Mentions: All subjects who participated in this study were healthy and without any chronic disease or daily intake of medicines. The characteristics of the subjects and the detection of MS by MSB agar medium, modified MSB agar medium, Dentocult SMTM and CRTTM are shown in Table 1. Experience of dental caries was as follows: number of filled teeth ranged from 0–27, missing teeth from 0–2, decayed teeth 0 (by oral examination). Figure 1 summarizes the estimation of MS which showed the different corresponding values or scores of MSB agar plate, Dentocult SMTM and CRTTM for the three of nine subjects. Levels of MS were compared among MSB agar medium and Dentocult SMTM or CRTTM and this is shown in Figure 2a–b. There was a positive association between MSB agar medium and Dentocult SMTM (P<.05, rs=0.53), whereas MSB agar medium and CRTTM showed no correlation (P<.05, rs=0.30).


Evaluation of cariogenic bacteria.

Nishikawara F, Nomura Y, Imai S, Senda A, Hanada N - Eur J Dent (2007)

Detection of MS was compared between MSB agar medium and Dentocult SMTM.The detection limits for MS on MSB agar plates were 0.Score for Dentocult SMTM and CRTTM are as follows, score 3: >6 log10CFU/ml, score 2: 6-5 log10CFU/ml, score 0-1: <5 log10CFU/ml.There was a positive association between MSB agar medium and Dentocult SMTM (P<.05, rs=0.53), whereas MSB agar medium and CRTTM showed no correlation (P<.05, rs=0.30) by Spearman.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2612953&req=5

f2a-0010031: Detection of MS was compared between MSB agar medium and Dentocult SMTM.The detection limits for MS on MSB agar plates were 0.Score for Dentocult SMTM and CRTTM are as follows, score 3: >6 log10CFU/ml, score 2: 6-5 log10CFU/ml, score 0-1: <5 log10CFU/ml.There was a positive association between MSB agar medium and Dentocult SMTM (P<.05, rs=0.53), whereas MSB agar medium and CRTTM showed no correlation (P<.05, rs=0.30) by Spearman.
Mentions: All subjects who participated in this study were healthy and without any chronic disease or daily intake of medicines. The characteristics of the subjects and the detection of MS by MSB agar medium, modified MSB agar medium, Dentocult SMTM and CRTTM are shown in Table 1. Experience of dental caries was as follows: number of filled teeth ranged from 0–27, missing teeth from 0–2, decayed teeth 0 (by oral examination). Figure 1 summarizes the estimation of MS which showed the different corresponding values or scores of MSB agar plate, Dentocult SMTM and CRTTM for the three of nine subjects. Levels of MS were compared among MSB agar medium and Dentocult SMTM or CRTTM and this is shown in Figure 2a–b. There was a positive association between MSB agar medium and Dentocult SMTM (P<.05, rs=0.53), whereas MSB agar medium and CRTTM showed no correlation (P<.05, rs=0.30).

Bottom Line: The ratios of MS in detected bacteria were compared by ELISA.The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRT(TM), (P< .01) and Dentocult SM(TM) (P<.05).The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium.

View Article: PubMed Central - PubMed

Affiliation: Department of Preventive Dentistry and Public Health, School of Dental Medicine, Tsurumi University,Japan.

ABSTRACT

Objectives: The evaluation of Mutans streptococci (MS) is one of the index for caries risk. Dentocult(TM) and CRT(TM) are commercial kits to detect and evaluate MS, conveniently. However, the evaluation of MS has also been carried out simply using an instruction manual. But the instruction manual is not easy to use for evaluation of MS. The aim of this study was to examine the utility of modified Mitis-Salivalius Bacitracin (MSB) agar medium compared with MSB agar medium and commercial kits, and to establish a convenient kit (mMSB-kit) using modified MSB agar.

Methods: The MS in stimulated saliva from 27 subjects were detected by MSB, modified MSB agar medium and commercial kits. Laboratory and clinically isolated strains of MS were similarly evaluated. The ratios of MS in detected bacteria were compared by ELISA.

Results: The scores using an mMSB-kit on the basis of modified MSB agar medium were tabulated. Saliva samples showed different levels of MS between culture methods and the commercial kit. Some samples which were full of MS were not detected by the commercial kit. The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRT(TM), (P< .01) and Dentocult SM(TM) (P<.05).

Conclusion: The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium. The mMSB-kit can be used simply, and can be an important contributor for the evaluation of MS as a caries risk factor.

No MeSH data available.


Related in: MedlinePlus