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Detection of Enterococcus faecalis in Necrotic Teeth Root Canals by Culture and Polymerase Chain Reaction Methods.

Cogulu D, Uzel A, Oncag O, Aksoy SC, Eronat C - Eur J Dent (2007)

Bottom Line: A total of 145 children aged 5-13 years old were involved in this study.Statistically significant difference in the presence of E. faecalis in deciduous and permanent teeth was found by culture and PCR methods (P=0.03 and 0.02, respectively).The difference in the presence of E. faecalis between two different methods was not statistically significant (P>.05).

View Article: PubMed Central - PubMed

Affiliation: Ege University, School of Dentistry, Department of Pedodontics, Bornova-Izmir,TURKEY.

ABSTRACT

Objectives: The aim of this study was to investigate the presence of Enterococcus faecalis in endodontic infections in both deciduous and permanent teeth by culture and polymerase chain reaction (PCR) methods.

Methods: A total of 145 children aged 5-13 years old were involved in this study. The presence of E. faecalis in necrotic deciduous and permanent teeth root canals was studied using culture and polymerase chain reaction methods.

Results: Among 145 molar teeth, 57% (n=83) presented necrotic asymptomatic pulp tissues and were included in this study. Culture and PCR methods detected the test species in 18 and 22 of 83 teeth involved, respectively. E. faecalis was cultured from 8 (18%) of 45 necrotic deciduous teeth and from 10 (26%) of 38 necrotic permanent teeth. PCR detection identified the target species in 10 (22%) and 12 (32%) of necrotic deciduous and permanent teeth respectively. Statistically significant difference in the presence of E. faecalis in deciduous and permanent teeth was found by culture and PCR methods (P=0.03 and 0.02, respectively). The difference in the presence of E. faecalis between two different methods was not statistically significant (P>.05).

Conclusions: The results of the present study confirm that both culture and PCR methods are sensitive to detect E. faecalis in root canals.

No MeSH data available.


Related in: MedlinePlus

Detection of E. faecalis by PCR.M: Marker DNA (GeneRuler®DNA Ladder Mix (Fermantas))1–10: E.faecalis positive samples
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f1-0010216: Detection of E. faecalis by PCR.M: Marker DNA (GeneRuler®DNA Ladder Mix (Fermantas))1–10: E.faecalis positive samples

Mentions: DNA amplification was performed in a thermal cycler Gene Amp®PCR system (Applied Biosystems). Amplicons were stored at −20°C. The amplification products were analyzed through the use of electrophoresis in a 1.5% agarose gel conducted at 4V/cm in Tris-borate EDTA buffer. The gels were stained with 0.5 μg/ml ethidium bromide and the PCR products were visualized under 300 nm ultraviolet light. GeneRuler®DNA Ladder Mix (Fermentas GmbH, Germany).served as the molecular weight marker. The identity of each band was determined by visual comparison with a molecular weight ladder. Reactions were deemed positive in the presence of bands of the appropriate size (Figure 1).


Detection of Enterococcus faecalis in Necrotic Teeth Root Canals by Culture and Polymerase Chain Reaction Methods.

Cogulu D, Uzel A, Oncag O, Aksoy SC, Eronat C - Eur J Dent (2007)

Detection of E. faecalis by PCR.M: Marker DNA (GeneRuler®DNA Ladder Mix (Fermantas))1–10: E.faecalis positive samples
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2609913&req=5

f1-0010216: Detection of E. faecalis by PCR.M: Marker DNA (GeneRuler®DNA Ladder Mix (Fermantas))1–10: E.faecalis positive samples
Mentions: DNA amplification was performed in a thermal cycler Gene Amp®PCR system (Applied Biosystems). Amplicons were stored at −20°C. The amplification products were analyzed through the use of electrophoresis in a 1.5% agarose gel conducted at 4V/cm in Tris-borate EDTA buffer. The gels were stained with 0.5 μg/ml ethidium bromide and the PCR products were visualized under 300 nm ultraviolet light. GeneRuler®DNA Ladder Mix (Fermentas GmbH, Germany).served as the molecular weight marker. The identity of each band was determined by visual comparison with a molecular weight ladder. Reactions were deemed positive in the presence of bands of the appropriate size (Figure 1).

Bottom Line: A total of 145 children aged 5-13 years old were involved in this study.Statistically significant difference in the presence of E. faecalis in deciduous and permanent teeth was found by culture and PCR methods (P=0.03 and 0.02, respectively).The difference in the presence of E. faecalis between two different methods was not statistically significant (P>.05).

View Article: PubMed Central - PubMed

Affiliation: Ege University, School of Dentistry, Department of Pedodontics, Bornova-Izmir,TURKEY.

ABSTRACT

Objectives: The aim of this study was to investigate the presence of Enterococcus faecalis in endodontic infections in both deciduous and permanent teeth by culture and polymerase chain reaction (PCR) methods.

Methods: A total of 145 children aged 5-13 years old were involved in this study. The presence of E. faecalis in necrotic deciduous and permanent teeth root canals was studied using culture and polymerase chain reaction methods.

Results: Among 145 molar teeth, 57% (n=83) presented necrotic asymptomatic pulp tissues and were included in this study. Culture and PCR methods detected the test species in 18 and 22 of 83 teeth involved, respectively. E. faecalis was cultured from 8 (18%) of 45 necrotic deciduous teeth and from 10 (26%) of 38 necrotic permanent teeth. PCR detection identified the target species in 10 (22%) and 12 (32%) of necrotic deciduous and permanent teeth respectively. Statistically significant difference in the presence of E. faecalis in deciduous and permanent teeth was found by culture and PCR methods (P=0.03 and 0.02, respectively). The difference in the presence of E. faecalis between two different methods was not statistically significant (P>.05).

Conclusions: The results of the present study confirm that both culture and PCR methods are sensitive to detect E. faecalis in root canals.

No MeSH data available.


Related in: MedlinePlus