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New hosts for equine herpesvirus 9.

Schrenzel MD, Tucker TA, Donovan TA, Busch MD, Wise AG, Maes RK, Kiupel M - Emerging Infect. Dis. (2008)

Bottom Line: Equine herpesvirus 9 was detected in a polar bear with progressive encephalitis; the source was traced to 2 members of a potential equid reservoir species, Grevy's zebras.The virus was also found in an aborted Persian onager.Thus, the natural host range is extended to 6 species in 3 mammalian orders.

View Article: PubMed Central - PubMed

Affiliation: Zoological Society of San Diego, Wildlife Disease Laboratories, Escondido, California 92027, USA. mschrenzel@sandiegozoo.org

ABSTRACT
Equine herpesvirus 9 was detected in a polar bear with progressive encephalitis; the source was traced to 2 members of a potential equid reservoir species, Grevy's zebras. The virus was also found in an aborted Persian onager. Thus, the natural host range is extended to 6 species in 3 mammalian orders.

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Related in: MedlinePlus

Photomicrographs showing A) encephalitis with neuronal necrosis and intranuclear inclusions (arrows) in a polar bear (Ursus maritimus); scale bar = 50 μm; hematoxylin and eosin stain; and B) Grevy’s zebra (Equus grevysi) with acute rhinitis with eosinophilic inclusions in respiratory epithelium; scale bar = 20 μm; hematoxylin and eosin stain.
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Figure 1: Photomicrographs showing A) encephalitis with neuronal necrosis and intranuclear inclusions (arrows) in a polar bear (Ursus maritimus); scale bar = 50 μm; hematoxylin and eosin stain; and B) Grevy’s zebra (Equus grevysi) with acute rhinitis with eosinophilic inclusions in respiratory epithelium; scale bar = 20 μm; hematoxylin and eosin stain.

Mentions: In July, 2007, a 12-year-old polar bear (Ursus maritimus) in a zoological garden in San Diego, California, showed progressive neurologic signs that were refractory to therapy. The animal was housed ≈200 feet from a herd of recently relocated Grevy’s zebras (E. grevysi). Ultimately, the bear was euthanized. Postmortem examination showed nonsuppurative meningoencephalitis with neuronal and glial intranuclear inclusion bodies (Figure 1, panel A). PCR targeting of conserved regions of herpesvirus DNA polymerase genes identified a virus in the brain with 98% homology to EHV-1 in a 165-bp segment of DNA (2,7). Subsequently, 2 other PCRs targeting an additional 742 bp of the DNA polymerase gene (sense primer 5′-GCATYWTCCCCCCGTTKATRAC-3′ and antisense primer 5′-ATAGYSAARRCCACGCCTTY-3′) and 1,181 bp of the glycoprotein B (gB) gene (sense primer 5′-CTTGTGAGATCTAACCGCAC-3′ and antisense primer 5′-GGGTATAGAGCTTTCATGGGG-3′) identified the virus as EHV-9 and enabled more precise strain determination (2). DNA segments of the terminase and gB genes were also characterized to generate additional phylograms and to compare with molecular findings from other animals in this study (8,9).


New hosts for equine herpesvirus 9.

Schrenzel MD, Tucker TA, Donovan TA, Busch MD, Wise AG, Maes RK, Kiupel M - Emerging Infect. Dis. (2008)

Photomicrographs showing A) encephalitis with neuronal necrosis and intranuclear inclusions (arrows) in a polar bear (Ursus maritimus); scale bar = 50 μm; hematoxylin and eosin stain; and B) Grevy’s zebra (Equus grevysi) with acute rhinitis with eosinophilic inclusions in respiratory epithelium; scale bar = 20 μm; hematoxylin and eosin stain.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2609862&req=5

Figure 1: Photomicrographs showing A) encephalitis with neuronal necrosis and intranuclear inclusions (arrows) in a polar bear (Ursus maritimus); scale bar = 50 μm; hematoxylin and eosin stain; and B) Grevy’s zebra (Equus grevysi) with acute rhinitis with eosinophilic inclusions in respiratory epithelium; scale bar = 20 μm; hematoxylin and eosin stain.
Mentions: In July, 2007, a 12-year-old polar bear (Ursus maritimus) in a zoological garden in San Diego, California, showed progressive neurologic signs that were refractory to therapy. The animal was housed ≈200 feet from a herd of recently relocated Grevy’s zebras (E. grevysi). Ultimately, the bear was euthanized. Postmortem examination showed nonsuppurative meningoencephalitis with neuronal and glial intranuclear inclusion bodies (Figure 1, panel A). PCR targeting of conserved regions of herpesvirus DNA polymerase genes identified a virus in the brain with 98% homology to EHV-1 in a 165-bp segment of DNA (2,7). Subsequently, 2 other PCRs targeting an additional 742 bp of the DNA polymerase gene (sense primer 5′-GCATYWTCCCCCCGTTKATRAC-3′ and antisense primer 5′-ATAGYSAARRCCACGCCTTY-3′) and 1,181 bp of the glycoprotein B (gB) gene (sense primer 5′-CTTGTGAGATCTAACCGCAC-3′ and antisense primer 5′-GGGTATAGAGCTTTCATGGGG-3′) identified the virus as EHV-9 and enabled more precise strain determination (2). DNA segments of the terminase and gB genes were also characterized to generate additional phylograms and to compare with molecular findings from other animals in this study (8,9).

Bottom Line: Equine herpesvirus 9 was detected in a polar bear with progressive encephalitis; the source was traced to 2 members of a potential equid reservoir species, Grevy's zebras.The virus was also found in an aborted Persian onager.Thus, the natural host range is extended to 6 species in 3 mammalian orders.

View Article: PubMed Central - PubMed

Affiliation: Zoological Society of San Diego, Wildlife Disease Laboratories, Escondido, California 92027, USA. mschrenzel@sandiegozoo.org

ABSTRACT
Equine herpesvirus 9 was detected in a polar bear with progressive encephalitis; the source was traced to 2 members of a potential equid reservoir species, Grevy's zebras. The virus was also found in an aborted Persian onager. Thus, the natural host range is extended to 6 species in 3 mammalian orders.

Show MeSH
Related in: MedlinePlus