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Endemic and epidemic lineages of Escherichia coli that cause urinary tract infections.

Manges AR, Tabor H, Tellis P, Vincent C, Tellier PP - Emerging Infect. Dis. (2008)

Bottom Line: We compared strains of UTI-causing E. coli in California with strains causing such infections in Montréal, Québec, Canada.Two clonal groups were identified in both locations.Epidemic transmission followed by endemic transmission of UTI-causing clonal groups may explain these clusters of UTI cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Epidemiology, Biostatistics and Occupational Health, McGill University, Montréal, Québec, Canada. amee.manges@mcgill.ca

ABSTRACT
Women with urinary tract infections (UTIs) in California, USA (1999-2001), were infected with closely related or indistinguishable strains of Escherichia coli (clonal groups), which suggests point source dissemination. We compared strains of UTI-causing E. coli in California with strains causing such infections in Montréal, Québec, Canada. Urine specimens from women with community-acquired UTIs in Montréal (2006) were cultured for E. coli. Isolates that caused 256 consecutive episodes of UTI were characterized by antimicrobial drug susceptibility profile, enterobacterial repetitive intergenic consensus 2 PCR, serotyping, XbaI and NotI pulsed-field gel electrophoresis, multilocus sequence typing, and phylogenetic typing. We confirmed the presence of drug-resistant, genetically related, and temporally clustered E. coli clonal groups that caused community-acquired UTIs in unrelated women in 2 locations and 2 different times. Two clonal groups were identified in both locations. Epidemic transmission followed by endemic transmission of UTI-causing clonal groups may explain these clusters of UTI cases.

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Related in: MedlinePlus

XbaI and NotI pulsed-field gel electrophoresis patterns for clonal group I Escherichia coli isolated from women with urinary tract infections in Montréal, Québec, Canada, 2006. The 6 isolates shown were resistant to ciprofloxacin and in serogroup O25:H4. First and last lanes, bacteriophage λ; lane +, positive control.
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Figure 2: XbaI and NotI pulsed-field gel electrophoresis patterns for clonal group I Escherichia coli isolated from women with urinary tract infections in Montréal, Québec, Canada, 2006. The 6 isolates shown were resistant to ciprofloxacin and in serogroup O25:H4. First and last lanes, bacteriophage λ; lane +, positive control.

Mentions: PFGE confirmed the presence of 4 clonal groups among the Montréal isolates. CgH was found only in Montréal and showed indistinguishable XbaI and NotI PFGE patterns (Figure 1). CgI was also found only in Montréal and could be considered possibly related by the criteria of Tenover et al. (26) (Figure 2). Patterns of CgC isolates (Figure 3) identified in California and Montréal differed by <6 bands, regardless of restriction enzyme used. The PFGE results for CgA varied the most among all clonal groups from Montréal; in some cases, the PFGE patterns showed >6 band differences (Figure 4).


Endemic and epidemic lineages of Escherichia coli that cause urinary tract infections.

Manges AR, Tabor H, Tellis P, Vincent C, Tellier PP - Emerging Infect. Dis. (2008)

XbaI and NotI pulsed-field gel electrophoresis patterns for clonal group I Escherichia coli isolated from women with urinary tract infections in Montréal, Québec, Canada, 2006. The 6 isolates shown were resistant to ciprofloxacin and in serogroup O25:H4. First and last lanes, bacteriophage λ; lane +, positive control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2609861&req=5

Figure 2: XbaI and NotI pulsed-field gel electrophoresis patterns for clonal group I Escherichia coli isolated from women with urinary tract infections in Montréal, Québec, Canada, 2006. The 6 isolates shown were resistant to ciprofloxacin and in serogroup O25:H4. First and last lanes, bacteriophage λ; lane +, positive control.
Mentions: PFGE confirmed the presence of 4 clonal groups among the Montréal isolates. CgH was found only in Montréal and showed indistinguishable XbaI and NotI PFGE patterns (Figure 1). CgI was also found only in Montréal and could be considered possibly related by the criteria of Tenover et al. (26) (Figure 2). Patterns of CgC isolates (Figure 3) identified in California and Montréal differed by <6 bands, regardless of restriction enzyme used. The PFGE results for CgA varied the most among all clonal groups from Montréal; in some cases, the PFGE patterns showed >6 band differences (Figure 4).

Bottom Line: We compared strains of UTI-causing E. coli in California with strains causing such infections in Montréal, Québec, Canada.Two clonal groups were identified in both locations.Epidemic transmission followed by endemic transmission of UTI-causing clonal groups may explain these clusters of UTI cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Epidemiology, Biostatistics and Occupational Health, McGill University, Montréal, Québec, Canada. amee.manges@mcgill.ca

ABSTRACT
Women with urinary tract infections (UTIs) in California, USA (1999-2001), were infected with closely related or indistinguishable strains of Escherichia coli (clonal groups), which suggests point source dissemination. We compared strains of UTI-causing E. coli in California with strains causing such infections in Montréal, Québec, Canada. Urine specimens from women with community-acquired UTIs in Montréal (2006) were cultured for E. coli. Isolates that caused 256 consecutive episodes of UTI were characterized by antimicrobial drug susceptibility profile, enterobacterial repetitive intergenic consensus 2 PCR, serotyping, XbaI and NotI pulsed-field gel electrophoresis, multilocus sequence typing, and phylogenetic typing. We confirmed the presence of drug-resistant, genetically related, and temporally clustered E. coli clonal groups that caused community-acquired UTIs in unrelated women in 2 locations and 2 different times. Two clonal groups were identified in both locations. Epidemic transmission followed by endemic transmission of UTI-causing clonal groups may explain these clusters of UTI cases.

Show MeSH
Related in: MedlinePlus