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Mast cells and angiogenesis in oral malignant and premalignant lesions.

Michailidou EZ, Markopoulos AK, Antoniades DZ - Open Dent J (2008)

Bottom Line: Toluidine blue counterstaining revealed mast cells.MVD was found to depend on MCD (p=0.000) in a percent 28.3% (power curve fit model).Mast cells are attracted at the lesion site and may turn on an angiogenic switch during tumorigenesis in OSCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Medicine and Maxillofacial Pathology, Aristotle University, Thessaloniki, Greece.

ABSTRACT

Unlabelled: Mast cell contribution to neoangiogenesis during tumorigenesis in oral squamous cell carcinoma is not determined yet.

Objectives: To associate numerical mast cell density (MCD) to numerical microvessel density (MVD) during the progression of oral leukoplakia without dysplasia and leukoplakia with dysplasia to squamous cell carcinoma (OSCC).

Materials and methods: MVD was analysed immunohistochemically (mouse monoclonal anti-human CD34) in 49 paraffin-embedded specimens, 35 OSCCs, 9 leukoplakias and 5 normal oral tissues. Toluidine blue counterstaining revealed mast cells. MCD and MVD were assessed at the same optical field.

Results: MVD increased between: normal oral mucosa, dysplasia (p=0.004), OSCC (p=0.001), leukoplakia and OSCC (p=0.041). MCD increased between: normal oral mucosa, dysplasia (p=0.003), OSCC (p=0.000), leukoplakia and OSCC (p=0.007). MVD was found to depend on MCD (p=0.000) in a percent 28.3% (power curve fit model).

Conclusions: Mast cells are attracted at the lesion site and may turn on an angiogenic switch during tumorigenesis in OSCC.

No MeSH data available.


Related in: MedlinePlus

MVD (red arrow) and MCD (green arrow) were low in oral leukoplakia without dysplasia similar to those of normal oral epithelium. (CD34 & toluidine staining, original magnification X200).
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Figure 2: MVD (red arrow) and MCD (green arrow) were low in oral leukoplakia without dysplasia similar to those of normal oral epithelium. (CD34 & toluidine staining, original magnification X200).

Mentions: In normal oral epithelium, leukoplakia without dysplasia and leukoplakia with dysplasia lesions, microvessels are mainly located just underneath the epithelium and were stained as brown spots, lines or lumens. MVD was counted in every tissue. In normal oral tissues the mean MVD was 14.08, in leukoplakia without dysplasia 18.18, in leukoplakia with dysplasia 21.79 and in OSCC 24.78. The number of microvessels was found to be increased significantly between: Normal oral mucosa and leukoplakia with mild (p=0.022), and severe dysplasia (p=0.04) and also between normal oral mucosa and OSCC (p=0.000). The number of microvessels was also found to be increased significantly between: leukoplakia without dysplasia (p=0.042), leukoplakia with mild (p=0.033), moderate (p=0.010), severe dysplasia (p=0.04) and OSCC, but it was not significantly increased between leukoplakia with severe dysplasia and OSCC (p=0.262) (Table 1, Figs. 1,2,3,4). No correlation was found between the microvessel density and the age of the patients (mean= 22.8 and p=0.30) or the site of the primary tumor (mean=23 and p=0.247).


Mast cells and angiogenesis in oral malignant and premalignant lesions.

Michailidou EZ, Markopoulos AK, Antoniades DZ - Open Dent J (2008)

MVD (red arrow) and MCD (green arrow) were low in oral leukoplakia without dysplasia similar to those of normal oral epithelium. (CD34 & toluidine staining, original magnification X200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2606660&req=5

Figure 2: MVD (red arrow) and MCD (green arrow) were low in oral leukoplakia without dysplasia similar to those of normal oral epithelium. (CD34 & toluidine staining, original magnification X200).
Mentions: In normal oral epithelium, leukoplakia without dysplasia and leukoplakia with dysplasia lesions, microvessels are mainly located just underneath the epithelium and were stained as brown spots, lines or lumens. MVD was counted in every tissue. In normal oral tissues the mean MVD was 14.08, in leukoplakia without dysplasia 18.18, in leukoplakia with dysplasia 21.79 and in OSCC 24.78. The number of microvessels was found to be increased significantly between: Normal oral mucosa and leukoplakia with mild (p=0.022), and severe dysplasia (p=0.04) and also between normal oral mucosa and OSCC (p=0.000). The number of microvessels was also found to be increased significantly between: leukoplakia without dysplasia (p=0.042), leukoplakia with mild (p=0.033), moderate (p=0.010), severe dysplasia (p=0.04) and OSCC, but it was not significantly increased between leukoplakia with severe dysplasia and OSCC (p=0.262) (Table 1, Figs. 1,2,3,4). No correlation was found between the microvessel density and the age of the patients (mean= 22.8 and p=0.30) or the site of the primary tumor (mean=23 and p=0.247).

Bottom Line: Toluidine blue counterstaining revealed mast cells.MVD was found to depend on MCD (p=0.000) in a percent 28.3% (power curve fit model).Mast cells are attracted at the lesion site and may turn on an angiogenic switch during tumorigenesis in OSCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Medicine and Maxillofacial Pathology, Aristotle University, Thessaloniki, Greece.

ABSTRACT

Unlabelled: Mast cell contribution to neoangiogenesis during tumorigenesis in oral squamous cell carcinoma is not determined yet.

Objectives: To associate numerical mast cell density (MCD) to numerical microvessel density (MVD) during the progression of oral leukoplakia without dysplasia and leukoplakia with dysplasia to squamous cell carcinoma (OSCC).

Materials and methods: MVD was analysed immunohistochemically (mouse monoclonal anti-human CD34) in 49 paraffin-embedded specimens, 35 OSCCs, 9 leukoplakias and 5 normal oral tissues. Toluidine blue counterstaining revealed mast cells. MCD and MVD were assessed at the same optical field.

Results: MVD increased between: normal oral mucosa, dysplasia (p=0.004), OSCC (p=0.001), leukoplakia and OSCC (p=0.041). MCD increased between: normal oral mucosa, dysplasia (p=0.003), OSCC (p=0.000), leukoplakia and OSCC (p=0.007). MVD was found to depend on MCD (p=0.000) in a percent 28.3% (power curve fit model).

Conclusions: Mast cells are attracted at the lesion site and may turn on an angiogenic switch during tumorigenesis in OSCC.

No MeSH data available.


Related in: MedlinePlus