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Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines.

Lee do K, Jang S, Kim MJ, Kim JH, Chung MJ, Kim KJ, Ha NJ - BMC Cancer (2008)

Bottom Line: Lactic acid bacteria (LAB) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as anti-tumor activity.The effects of B. adolescentis SPM0212 butanol extract on tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) production were tested using the murine macrophage RAW 264.7 cell line.The butanol extract of B. adolescentis SPM0212 increased activity of the host immune system and may improve human health by helping to prevent colon cancer as a biological response modifier.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacy, Sahmyook University, Seoul 139-742, Republic of Korea. 015790@hanmail.net

ABSTRACT

Background: Lactic acid bacteria (LAB) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as anti-tumor activity. The aim of the present work was to study the growth inhibition of tumor cells by butanol extract of Bifidobacterium adolescentis isolated from healthy young Koreans.

Methods: The anti-proliferative activity of B. adolescentis isolates was assessed by XTT assays on three human colon cancer cell lines (Caco-2, HT-29, and SW480). The effects of B. adolescentis SPM0212 butanol extract on tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) production were tested using the murine macrophage RAW 264.7 cell line.

Results: The butanol extract of B. adolescentis SPM0212 dose-dependently inhibited the growth of Caco-2, HT-29, and SW480 cells by 70%, 30%, and 40%, respectively, at 200 microg/mL. Additionally, the butanol extract of B. adolescentis SPM0212 induced macrophage activation and significantly increased the production of TNF-alpha and NO, which regulate immune modulation and are cytotoxic to tumor cells.

Conclusion: The butanol extract of B. adolescentis SPM0212 increased activity of the host immune system and may improve human health by helping to prevent colon cancer as a biological response modifier.

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Related in: MedlinePlus

Characterization of RAW 264.7 cells in response to butanol extract of B. adolescentis SPM0212. RAW 264.7 cells (1 × 104 cells/well) were cultured on cover slips in the presence of different concentrations of butanol extract of B. adolescentis SPM0212 for 48 hr. The cells were fixed and stained in Diff-quick and observed under a light microscope at 400×. (A) Murine macrophage cells. (B) LPS (50 ng/ml). (C) Butanol extract of B. adolescentis SPM0212 (25 μg/ml). (D) Butanol extract of B. adolescentis SPM0212 (50 μg/ml). (E) Butanol extract of B. adolescentis SPM0212 (100 μg/ml). (F) Butanol extract of B. adolescentis SPM0212 (200 μg/ml).
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Figure 4: Characterization of RAW 264.7 cells in response to butanol extract of B. adolescentis SPM0212. RAW 264.7 cells (1 × 104 cells/well) were cultured on cover slips in the presence of different concentrations of butanol extract of B. adolescentis SPM0212 for 48 hr. The cells were fixed and stained in Diff-quick and observed under a light microscope at 400×. (A) Murine macrophage cells. (B) LPS (50 ng/ml). (C) Butanol extract of B. adolescentis SPM0212 (25 μg/ml). (D) Butanol extract of B. adolescentis SPM0212 (50 μg/ml). (E) Butanol extract of B. adolescentis SPM0212 (100 μg/ml). (F) Butanol extract of B. adolescentis SPM0212 (200 μg/ml).

Mentions: Normal RAW 264.7 cells, when cultured in medium alone, look refractile and rounded morphology and do not spread over the surface (Figure 4A). Activated macrophages usually display a distinct morphology, which is similar to the dendritic cell. Exposure to LPS (50 ng/ml; the positive control) induced morphological alteration of the RAW 264.7 cells (Figure 4B). Treatment with B. adolescentis SPM0212 butanol extract caused RAW 264.7 cells to become larger and rougher in a dose-dependent manner, suggesting activation (Figure 4C–F).


Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines.

Lee do K, Jang S, Kim MJ, Kim JH, Chung MJ, Kim KJ, Ha NJ - BMC Cancer (2008)

Characterization of RAW 264.7 cells in response to butanol extract of B. adolescentis SPM0212. RAW 264.7 cells (1 × 104 cells/well) were cultured on cover slips in the presence of different concentrations of butanol extract of B. adolescentis SPM0212 for 48 hr. The cells were fixed and stained in Diff-quick and observed under a light microscope at 400×. (A) Murine macrophage cells. (B) LPS (50 ng/ml). (C) Butanol extract of B. adolescentis SPM0212 (25 μg/ml). (D) Butanol extract of B. adolescentis SPM0212 (50 μg/ml). (E) Butanol extract of B. adolescentis SPM0212 (100 μg/ml). (F) Butanol extract of B. adolescentis SPM0212 (200 μg/ml).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2605768&req=5

Figure 4: Characterization of RAW 264.7 cells in response to butanol extract of B. adolescentis SPM0212. RAW 264.7 cells (1 × 104 cells/well) were cultured on cover slips in the presence of different concentrations of butanol extract of B. adolescentis SPM0212 for 48 hr. The cells were fixed and stained in Diff-quick and observed under a light microscope at 400×. (A) Murine macrophage cells. (B) LPS (50 ng/ml). (C) Butanol extract of B. adolescentis SPM0212 (25 μg/ml). (D) Butanol extract of B. adolescentis SPM0212 (50 μg/ml). (E) Butanol extract of B. adolescentis SPM0212 (100 μg/ml). (F) Butanol extract of B. adolescentis SPM0212 (200 μg/ml).
Mentions: Normal RAW 264.7 cells, when cultured in medium alone, look refractile and rounded morphology and do not spread over the surface (Figure 4A). Activated macrophages usually display a distinct morphology, which is similar to the dendritic cell. Exposure to LPS (50 ng/ml; the positive control) induced morphological alteration of the RAW 264.7 cells (Figure 4B). Treatment with B. adolescentis SPM0212 butanol extract caused RAW 264.7 cells to become larger and rougher in a dose-dependent manner, suggesting activation (Figure 4C–F).

Bottom Line: Lactic acid bacteria (LAB) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as anti-tumor activity.The effects of B. adolescentis SPM0212 butanol extract on tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) production were tested using the murine macrophage RAW 264.7 cell line.The butanol extract of B. adolescentis SPM0212 increased activity of the host immune system and may improve human health by helping to prevent colon cancer as a biological response modifier.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacy, Sahmyook University, Seoul 139-742, Republic of Korea. 015790@hanmail.net

ABSTRACT

Background: Lactic acid bacteria (LAB) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as anti-tumor activity. The aim of the present work was to study the growth inhibition of tumor cells by butanol extract of Bifidobacterium adolescentis isolated from healthy young Koreans.

Methods: The anti-proliferative activity of B. adolescentis isolates was assessed by XTT assays on three human colon cancer cell lines (Caco-2, HT-29, and SW480). The effects of B. adolescentis SPM0212 butanol extract on tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) production were tested using the murine macrophage RAW 264.7 cell line.

Results: The butanol extract of B. adolescentis SPM0212 dose-dependently inhibited the growth of Caco-2, HT-29, and SW480 cells by 70%, 30%, and 40%, respectively, at 200 microg/mL. Additionally, the butanol extract of B. adolescentis SPM0212 induced macrophage activation and significantly increased the production of TNF-alpha and NO, which regulate immune modulation and are cytotoxic to tumor cells.

Conclusion: The butanol extract of B. adolescentis SPM0212 increased activity of the host immune system and may improve human health by helping to prevent colon cancer as a biological response modifier.

Show MeSH
Related in: MedlinePlus