Limits...
Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture.

Jacchetti E, Emilitri E, Rodighiero S, Indrieri M, Gianfelice A, Lenardi C, Podestà A, Ranucci E, Ferruti P, Milani P - J Nanobiotechnology (2008)

Bottom Line: The cell adhesion on the agmatine containing substrates was comparable to that on plastic substrates and significantly enhanced with respect to the non-functionalized controls.In order to favor the handling of the samples, a procedure for the production of bi-layered constructs was also developed by means the deposition via spin coating of a thin layer of hydrogel on a pre-treated cover slip.In particular the incorporation of agmatine warrants good potential in the field of cell culturing and the development of supported functionalized hydrogels on cover glass are very promising substrates for applications in cell screening devices.

View Article: PubMed Central - HTML - PubMed

Affiliation: Istituto di Fisiologia Generale e Chimica Biologica, Università di Milano, via Trentacoste 2, 20134 Milano, Italy. cristina.lenardi@mi.infn.it.

ABSTRACT

Background: Poly(amidoamine)s (PAAs) are synthetic polymers endowed with many biologically interesting properties, being highly biocompatible, non toxic and biodegradable. Hydrogels based on PAAs can be easily modified during the synthesis by the introduction of functional co-monomers. Aim of this work is the development and testing of novel amphoteric nanosized poly(amidoamine) hydrogel film incorporating 4-aminobutylguanidine (agmatine) moieties to create RGD-mimicking repeating units for promoting cell adhesion.

Results: A systematic comparative study of the response of an epithelial cell line was performed on hydrogels with agmatine and on non-functionalized amphoteric poly(amidoamine) hydrogels and tissue culture plastic substrates. The cell adhesion on the agmatine containing substrates was comparable to that on plastic substrates and significantly enhanced with respect to the non-functionalized controls. Interestingly, spreading and proliferation on the functionalized supports are slower than on plastic exhibiting the possibility of an easier control of the cell growth kinetics. In order to favor the handling of the samples, a procedure for the production of bi-layered constructs was also developed by means the deposition via spin coating of a thin layer of hydrogel on a pre-treated cover slip.

Conclusion: The obtained results reveal that PAAs hydrogels can be profitably functionalized and, in general, undergo physical and chemical modifications to meet specific requirements. In particular the incorporation of agmatine warrants good potential in the field of cell culturing and the development of supported functionalized hydrogels on cover glass are very promising substrates for applications in cell screening devices.

No MeSH data available.


Related in: MedlinePlus

Cell adhesion inhibition. The presence in the medium of a soluble polymer bearing the agmatine-BAC sequence in the repeating unit is able to prevent cell adhesion on all the tested substrates. 1 mM GRGD peptide and 1 mM AGMA1 (calculated on repeating unit concentration) have the same effect on cell adhesion inhibition, increasing the soluble polymer concentration to 10 mM does not increase significantly the inhibition of cell adhesion. On ISA23-75 the cells do not adhered in the presence of 10 mM AGMA1 in the medium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2605745&req=5

Figure 11: Cell adhesion inhibition. The presence in the medium of a soluble polymer bearing the agmatine-BAC sequence in the repeating unit is able to prevent cell adhesion on all the tested substrates. 1 mM GRGD peptide and 1 mM AGMA1 (calculated on repeating unit concentration) have the same effect on cell adhesion inhibition, increasing the soluble polymer concentration to 10 mM does not increase significantly the inhibition of cell adhesion. On ISA23-75 the cells do not adhered in the presence of 10 mM AGMA1 in the medium.

Mentions: The presence in the medium of a soluble polymer obtained by copolymerization of BAC and agmatine [29] up to a concentration of 1 mM in repeating units, proved to prevent cell adhesion on all the substrates (see Figure 11). Increasing the AGMA1 concentration up to 10 mM did not significantly increase the inhibition of cell adhesion, suggesting that the interested receptors are already almost completely saturated at 1 mM AGMA1. 1 mM GRGD peptide and 1 mM AGMA1 (calculated on repeating unit concentration) have the same effect on cell adhesion inhibition.


Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture.

Jacchetti E, Emilitri E, Rodighiero S, Indrieri M, Gianfelice A, Lenardi C, Podestà A, Ranucci E, Ferruti P, Milani P - J Nanobiotechnology (2008)

Cell adhesion inhibition. The presence in the medium of a soluble polymer bearing the agmatine-BAC sequence in the repeating unit is able to prevent cell adhesion on all the tested substrates. 1 mM GRGD peptide and 1 mM AGMA1 (calculated on repeating unit concentration) have the same effect on cell adhesion inhibition, increasing the soluble polymer concentration to 10 mM does not increase significantly the inhibition of cell adhesion. On ISA23-75 the cells do not adhered in the presence of 10 mM AGMA1 in the medium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2605745&req=5

Figure 11: Cell adhesion inhibition. The presence in the medium of a soluble polymer bearing the agmatine-BAC sequence in the repeating unit is able to prevent cell adhesion on all the tested substrates. 1 mM GRGD peptide and 1 mM AGMA1 (calculated on repeating unit concentration) have the same effect on cell adhesion inhibition, increasing the soluble polymer concentration to 10 mM does not increase significantly the inhibition of cell adhesion. On ISA23-75 the cells do not adhered in the presence of 10 mM AGMA1 in the medium.
Mentions: The presence in the medium of a soluble polymer obtained by copolymerization of BAC and agmatine [29] up to a concentration of 1 mM in repeating units, proved to prevent cell adhesion on all the substrates (see Figure 11). Increasing the AGMA1 concentration up to 10 mM did not significantly increase the inhibition of cell adhesion, suggesting that the interested receptors are already almost completely saturated at 1 mM AGMA1. 1 mM GRGD peptide and 1 mM AGMA1 (calculated on repeating unit concentration) have the same effect on cell adhesion inhibition.

Bottom Line: The cell adhesion on the agmatine containing substrates was comparable to that on plastic substrates and significantly enhanced with respect to the non-functionalized controls.In order to favor the handling of the samples, a procedure for the production of bi-layered constructs was also developed by means the deposition via spin coating of a thin layer of hydrogel on a pre-treated cover slip.In particular the incorporation of agmatine warrants good potential in the field of cell culturing and the development of supported functionalized hydrogels on cover glass are very promising substrates for applications in cell screening devices.

View Article: PubMed Central - HTML - PubMed

Affiliation: Istituto di Fisiologia Generale e Chimica Biologica, Università di Milano, via Trentacoste 2, 20134 Milano, Italy. cristina.lenardi@mi.infn.it.

ABSTRACT

Background: Poly(amidoamine)s (PAAs) are synthetic polymers endowed with many biologically interesting properties, being highly biocompatible, non toxic and biodegradable. Hydrogels based on PAAs can be easily modified during the synthesis by the introduction of functional co-monomers. Aim of this work is the development and testing of novel amphoteric nanosized poly(amidoamine) hydrogel film incorporating 4-aminobutylguanidine (agmatine) moieties to create RGD-mimicking repeating units for promoting cell adhesion.

Results: A systematic comparative study of the response of an epithelial cell line was performed on hydrogels with agmatine and on non-functionalized amphoteric poly(amidoamine) hydrogels and tissue culture plastic substrates. The cell adhesion on the agmatine containing substrates was comparable to that on plastic substrates and significantly enhanced with respect to the non-functionalized controls. Interestingly, spreading and proliferation on the functionalized supports are slower than on plastic exhibiting the possibility of an easier control of the cell growth kinetics. In order to favor the handling of the samples, a procedure for the production of bi-layered constructs was also developed by means the deposition via spin coating of a thin layer of hydrogel on a pre-treated cover slip.

Conclusion: The obtained results reveal that PAAs hydrogels can be profitably functionalized and, in general, undergo physical and chemical modifications to meet specific requirements. In particular the incorporation of agmatine warrants good potential in the field of cell culturing and the development of supported functionalized hydrogels on cover glass are very promising substrates for applications in cell screening devices.

No MeSH data available.


Related in: MedlinePlus