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Improved Insulin Resistance and Lipid Metabolism by Cinnamon Extract through Activation of Peroxisome Proliferator-Activated Receptors.

Sheng X, Zhang Y, Gong Z, Huang C, Zang YQ - PPAR Res (2008)

Bottom Line: Cinnamon, a widely used spice in food preparation and traditional antidiabetic remedy, is found to activate PPARgamma and alpha, resulting in improved insulin resistance, reduced fasted glucose, FFA, LDL-c, and AST levels in high-caloric diet-induced obesity (DIO) and db/db mice in its water extract form.The transactivities of both full length and ligand-binding domain (LBD) of PPARgamma and PPARalpha are activated by cinnamon as evidenced by reporter gene assays.These data suggest that cinnamon in its water extract form can act as a dual activator of PPARgamma and alpha, and may be an alternative to PPARgamma activator in managing obesity-related diabetes and hyperlipidemia.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of CAS, Chinese Academy of Sciences, 319 Yue Yang Road, Shanghai 200031, China.

ABSTRACT
Peroxisome proliferator-activated receptors (PPARs) are transcriptional factors involved in the regulation of insulin resistance and adipogenesis. Cinnamon, a widely used spice in food preparation and traditional antidiabetic remedy, is found to activate PPARgamma and alpha, resulting in improved insulin resistance, reduced fasted glucose, FFA, LDL-c, and AST levels in high-caloric diet-induced obesity (DIO) and db/db mice in its water extract form. In vitro studies demonstrate that cinnamon increases the expression of peroxisome proliferator-activated receptors gamma and alpha (PPARgamma/alpha) and their target genes such as LPL, CD36, GLUT4, and ACO in 3T3-L1 adipocyte. The transactivities of both full length and ligand-binding domain (LBD) of PPARgamma and PPARalpha are activated by cinnamon as evidenced by reporter gene assays. These data suggest that cinnamon in its water extract form can act as a dual activator of PPARgamma and alpha, and may be an alternative to PPARgamma activator in managing obesity-related diabetes and hyperlipidemia.

No MeSH data available.


Related in: MedlinePlus

CE activated transactivities of PPARγ and PPARα. Full-length PPARγ or PPARα wascotransfected with PPRE-J3-TK-Luc reporter construct to 293T cell and treatedwith CE (0.2 mg/mL, 0.6 mg/mL), 1 μM of troglitazone or WY-14643 for 24 hours as positivecontrols. Rellina luc was used as a transfection efficiency control and therelative luciferase activities were measured against renilla luciferaseactivities. For LBD activity assay, pMCX-GAL4-LBD of PPARγ or α expressionconstructs were cotransfected with USAG × 4-TK-Luc into 293T usingthe same protocol as described above. The empty vectors were used as control. (a) Full-lengthPPARγ. (b) Full-length PPARα. (c) LBD PPARγ. (d) LBD PPARα. The results represent three independent experiments.Data are presented as mean ± SE. *P < .05, **P < .01.Tro: troglitazone; WY: WY14643.
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fig4: CE activated transactivities of PPARγ and PPARα. Full-length PPARγ or PPARα wascotransfected with PPRE-J3-TK-Luc reporter construct to 293T cell and treatedwith CE (0.2 mg/mL, 0.6 mg/mL), 1 μM of troglitazone or WY-14643 for 24 hours as positivecontrols. Rellina luc was used as a transfection efficiency control and therelative luciferase activities were measured against renilla luciferaseactivities. For LBD activity assay, pMCX-GAL4-LBD of PPARγ or α expressionconstructs were cotransfected with USAG × 4-TK-Luc into 293T usingthe same protocol as described above. The empty vectors were used as control. (a) Full-lengthPPARγ. (b) Full-length PPARα. (c) LBD PPARγ. (d) LBD PPARα. The results represent three independent experiments.Data are presented as mean ± SE. *P < .05, **P < .01.Tro: troglitazone; WY: WY14643.

Mentions: To test whetherCE could increase the transactivities of PPARγ and PPARα, full-length PPARγ and PPARαexpression plasmids were transfected into 293T cells for the reporter geneassays. We found that CE increased the transactivities of both PPARγ (P < .05) and PPARα (P < .01) in a dose-dependentmanner (see Figures 4(a) and 4(b)). In order to exclude the endogenous factors,ligand-binding domains (LBD) of PPARγ and PPARα were used to evaluate the reportergene activities. Results showed that the LBD activities of both PPARγ and PPARαwere also elevated (P < .05) (see Figures 4(c) and 4(d)). These datasuggest that CE is a dual activator of both PPARγ and PPARα.


Improved Insulin Resistance and Lipid Metabolism by Cinnamon Extract through Activation of Peroxisome Proliferator-Activated Receptors.

Sheng X, Zhang Y, Gong Z, Huang C, Zang YQ - PPAR Res (2008)

CE activated transactivities of PPARγ and PPARα. Full-length PPARγ or PPARα wascotransfected with PPRE-J3-TK-Luc reporter construct to 293T cell and treatedwith CE (0.2 mg/mL, 0.6 mg/mL), 1 μM of troglitazone or WY-14643 for 24 hours as positivecontrols. Rellina luc was used as a transfection efficiency control and therelative luciferase activities were measured against renilla luciferaseactivities. For LBD activity assay, pMCX-GAL4-LBD of PPARγ or α expressionconstructs were cotransfected with USAG × 4-TK-Luc into 293T usingthe same protocol as described above. The empty vectors were used as control. (a) Full-lengthPPARγ. (b) Full-length PPARα. (c) LBD PPARγ. (d) LBD PPARα. The results represent three independent experiments.Data are presented as mean ± SE. *P < .05, **P < .01.Tro: troglitazone; WY: WY14643.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2602825&req=5

fig4: CE activated transactivities of PPARγ and PPARα. Full-length PPARγ or PPARα wascotransfected with PPRE-J3-TK-Luc reporter construct to 293T cell and treatedwith CE (0.2 mg/mL, 0.6 mg/mL), 1 μM of troglitazone or WY-14643 for 24 hours as positivecontrols. Rellina luc was used as a transfection efficiency control and therelative luciferase activities were measured against renilla luciferaseactivities. For LBD activity assay, pMCX-GAL4-LBD of PPARγ or α expressionconstructs were cotransfected with USAG × 4-TK-Luc into 293T usingthe same protocol as described above. The empty vectors were used as control. (a) Full-lengthPPARγ. (b) Full-length PPARα. (c) LBD PPARγ. (d) LBD PPARα. The results represent three independent experiments.Data are presented as mean ± SE. *P < .05, **P < .01.Tro: troglitazone; WY: WY14643.
Mentions: To test whetherCE could increase the transactivities of PPARγ and PPARα, full-length PPARγ and PPARαexpression plasmids were transfected into 293T cells for the reporter geneassays. We found that CE increased the transactivities of both PPARγ (P < .05) and PPARα (P < .01) in a dose-dependentmanner (see Figures 4(a) and 4(b)). In order to exclude the endogenous factors,ligand-binding domains (LBD) of PPARγ and PPARα were used to evaluate the reportergene activities. Results showed that the LBD activities of both PPARγ and PPARαwere also elevated (P < .05) (see Figures 4(c) and 4(d)). These datasuggest that CE is a dual activator of both PPARγ and PPARα.

Bottom Line: Cinnamon, a widely used spice in food preparation and traditional antidiabetic remedy, is found to activate PPARgamma and alpha, resulting in improved insulin resistance, reduced fasted glucose, FFA, LDL-c, and AST levels in high-caloric diet-induced obesity (DIO) and db/db mice in its water extract form.The transactivities of both full length and ligand-binding domain (LBD) of PPARgamma and PPARalpha are activated by cinnamon as evidenced by reporter gene assays.These data suggest that cinnamon in its water extract form can act as a dual activator of PPARgamma and alpha, and may be an alternative to PPARgamma activator in managing obesity-related diabetes and hyperlipidemia.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of CAS, Chinese Academy of Sciences, 319 Yue Yang Road, Shanghai 200031, China.

ABSTRACT
Peroxisome proliferator-activated receptors (PPARs) are transcriptional factors involved in the regulation of insulin resistance and adipogenesis. Cinnamon, a widely used spice in food preparation and traditional antidiabetic remedy, is found to activate PPARgamma and alpha, resulting in improved insulin resistance, reduced fasted glucose, FFA, LDL-c, and AST levels in high-caloric diet-induced obesity (DIO) and db/db mice in its water extract form. In vitro studies demonstrate that cinnamon increases the expression of peroxisome proliferator-activated receptors gamma and alpha (PPARgamma/alpha) and their target genes such as LPL, CD36, GLUT4, and ACO in 3T3-L1 adipocyte. The transactivities of both full length and ligand-binding domain (LBD) of PPARgamma and PPARalpha are activated by cinnamon as evidenced by reporter gene assays. These data suggest that cinnamon in its water extract form can act as a dual activator of PPARgamma and alpha, and may be an alternative to PPARgamma activator in managing obesity-related diabetes and hyperlipidemia.

No MeSH data available.


Related in: MedlinePlus