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Improved Insulin Resistance and Lipid Metabolism by Cinnamon Extract through Activation of Peroxisome Proliferator-Activated Receptors.

Sheng X, Zhang Y, Gong Z, Huang C, Zang YQ - PPAR Res (2008)

Bottom Line: Cinnamon, a widely used spice in food preparation and traditional antidiabetic remedy, is found to activate PPARgamma and alpha, resulting in improved insulin resistance, reduced fasted glucose, FFA, LDL-c, and AST levels in high-caloric diet-induced obesity (DIO) and db/db mice in its water extract form.The transactivities of both full length and ligand-binding domain (LBD) of PPARgamma and PPARalpha are activated by cinnamon as evidenced by reporter gene assays.These data suggest that cinnamon in its water extract form can act as a dual activator of PPARgamma and alpha, and may be an alternative to PPARgamma activator in managing obesity-related diabetes and hyperlipidemia.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of CAS, Chinese Academy of Sciences, 319 Yue Yang Road, Shanghai 200031, China.

ABSTRACT
Peroxisome proliferator-activated receptors (PPARs) are transcriptional factors involved in the regulation of insulin resistance and adipogenesis. Cinnamon, a widely used spice in food preparation and traditional antidiabetic remedy, is found to activate PPARgamma and alpha, resulting in improved insulin resistance, reduced fasted glucose, FFA, LDL-c, and AST levels in high-caloric diet-induced obesity (DIO) and db/db mice in its water extract form. In vitro studies demonstrate that cinnamon increases the expression of peroxisome proliferator-activated receptors gamma and alpha (PPARgamma/alpha) and their target genes such as LPL, CD36, GLUT4, and ACO in 3T3-L1 adipocyte. The transactivities of both full length and ligand-binding domain (LBD) of PPARgamma and PPARalpha are activated by cinnamon as evidenced by reporter gene assays. These data suggest that cinnamon in its water extract form can act as a dual activator of PPARgamma and alpha, and may be an alternative to PPARgamma activator in managing obesity-related diabetes and hyperlipidemia.

No MeSH data available.


Related in: MedlinePlus

CE increasedexpression of PPARs and their target genes in differentiated 3T3-L1 cells. 3T3-L1 cells weredifferentiated in 24-well plate and CE 0.6 mg/mL was added at the same time. Onday 5, cells were collected and total RNA was extracted and reverselytranscribed into the first strand cDNA with random hexamer primers using cDNA synthesis kit.The gene expression levels were analyzed by quantitative real-time RT-PCR. (a) PPARγand its target genes; (b) PPARα and its target gene; (c) Western blot of CE-treated3T3-L1 differentiated cells from day 5. 1: Control; 2: DM; 3: Rosiglitazone 1 μM; 4: DM + CE 0.2 mg/mL; 5: DM + CE 0.6 mg/mL. For real-time PCR, the resultswere repeated in at least 3 independent experiments, and β-actin mRNA was usedas an internal control. Data are presented as mean ± SE. *P < .001.
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fig3: CE increasedexpression of PPARs and their target genes in differentiated 3T3-L1 cells. 3T3-L1 cells weredifferentiated in 24-well plate and CE 0.6 mg/mL was added at the same time. Onday 5, cells were collected and total RNA was extracted and reverselytranscribed into the first strand cDNA with random hexamer primers using cDNA synthesis kit.The gene expression levels were analyzed by quantitative real-time RT-PCR. (a) PPARγand its target genes; (b) PPARα and its target gene; (c) Western blot of CE-treated3T3-L1 differentiated cells from day 5. 1: Control; 2: DM; 3: Rosiglitazone 1 μM; 4: DM + CE 0.2 mg/mL; 5: DM + CE 0.6 mg/mL. For real-time PCR, the resultswere repeated in at least 3 independent experiments, and β-actin mRNA was usedas an internal control. Data are presented as mean ± SE. *P < .001.

Mentions: Since PPARγ andPPARα play essential roles in adipocyte differentiation and lipid homeostasis, wefurther studied whether CE increases 3T3-L1 adipocyte differentiation through theactivation of PPARs. The above-mentioned CE-treated cells were collected todetermine the expression levels of PPARγ, PPARα and their target genes such as CD36,FAS, LPL, GLUT4, and ACO. The results showed that CE not only elevated theexpression of PPARγ and its target genes CD36, LPL, FAS, and GLUT4 significantly (see Figure 3(a)), but also increased the expression of PPARα and its target gene ACO markedly (Figure 3(b)). Further examination at the protein level of PPARγ confirmed that CE can significantly increase the PPARγ proteinlevel in 3T3-L1 adipocyte during differentiation (Figure 3(c)).


Improved Insulin Resistance and Lipid Metabolism by Cinnamon Extract through Activation of Peroxisome Proliferator-Activated Receptors.

Sheng X, Zhang Y, Gong Z, Huang C, Zang YQ - PPAR Res (2008)

CE increasedexpression of PPARs and their target genes in differentiated 3T3-L1 cells. 3T3-L1 cells weredifferentiated in 24-well plate and CE 0.6 mg/mL was added at the same time. Onday 5, cells were collected and total RNA was extracted and reverselytranscribed into the first strand cDNA with random hexamer primers using cDNA synthesis kit.The gene expression levels were analyzed by quantitative real-time RT-PCR. (a) PPARγand its target genes; (b) PPARα and its target gene; (c) Western blot of CE-treated3T3-L1 differentiated cells from day 5. 1: Control; 2: DM; 3: Rosiglitazone 1 μM; 4: DM + CE 0.2 mg/mL; 5: DM + CE 0.6 mg/mL. For real-time PCR, the resultswere repeated in at least 3 independent experiments, and β-actin mRNA was usedas an internal control. Data are presented as mean ± SE. *P < .001.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2602825&req=5

fig3: CE increasedexpression of PPARs and their target genes in differentiated 3T3-L1 cells. 3T3-L1 cells weredifferentiated in 24-well plate and CE 0.6 mg/mL was added at the same time. Onday 5, cells were collected and total RNA was extracted and reverselytranscribed into the first strand cDNA with random hexamer primers using cDNA synthesis kit.The gene expression levels were analyzed by quantitative real-time RT-PCR. (a) PPARγand its target genes; (b) PPARα and its target gene; (c) Western blot of CE-treated3T3-L1 differentiated cells from day 5. 1: Control; 2: DM; 3: Rosiglitazone 1 μM; 4: DM + CE 0.2 mg/mL; 5: DM + CE 0.6 mg/mL. For real-time PCR, the resultswere repeated in at least 3 independent experiments, and β-actin mRNA was usedas an internal control. Data are presented as mean ± SE. *P < .001.
Mentions: Since PPARγ andPPARα play essential roles in adipocyte differentiation and lipid homeostasis, wefurther studied whether CE increases 3T3-L1 adipocyte differentiation through theactivation of PPARs. The above-mentioned CE-treated cells were collected todetermine the expression levels of PPARγ, PPARα and their target genes such as CD36,FAS, LPL, GLUT4, and ACO. The results showed that CE not only elevated theexpression of PPARγ and its target genes CD36, LPL, FAS, and GLUT4 significantly (see Figure 3(a)), but also increased the expression of PPARα and its target gene ACO markedly (Figure 3(b)). Further examination at the protein level of PPARγ confirmed that CE can significantly increase the PPARγ proteinlevel in 3T3-L1 adipocyte during differentiation (Figure 3(c)).

Bottom Line: Cinnamon, a widely used spice in food preparation and traditional antidiabetic remedy, is found to activate PPARgamma and alpha, resulting in improved insulin resistance, reduced fasted glucose, FFA, LDL-c, and AST levels in high-caloric diet-induced obesity (DIO) and db/db mice in its water extract form.The transactivities of both full length and ligand-binding domain (LBD) of PPARgamma and PPARalpha are activated by cinnamon as evidenced by reporter gene assays.These data suggest that cinnamon in its water extract form can act as a dual activator of PPARgamma and alpha, and may be an alternative to PPARgamma activator in managing obesity-related diabetes and hyperlipidemia.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of CAS, Chinese Academy of Sciences, 319 Yue Yang Road, Shanghai 200031, China.

ABSTRACT
Peroxisome proliferator-activated receptors (PPARs) are transcriptional factors involved in the regulation of insulin resistance and adipogenesis. Cinnamon, a widely used spice in food preparation and traditional antidiabetic remedy, is found to activate PPARgamma and alpha, resulting in improved insulin resistance, reduced fasted glucose, FFA, LDL-c, and AST levels in high-caloric diet-induced obesity (DIO) and db/db mice in its water extract form. In vitro studies demonstrate that cinnamon increases the expression of peroxisome proliferator-activated receptors gamma and alpha (PPARgamma/alpha) and their target genes such as LPL, CD36, GLUT4, and ACO in 3T3-L1 adipocyte. The transactivities of both full length and ligand-binding domain (LBD) of PPARgamma and PPARalpha are activated by cinnamon as evidenced by reporter gene assays. These data suggest that cinnamon in its water extract form can act as a dual activator of PPARgamma and alpha, and may be an alternative to PPARgamma activator in managing obesity-related diabetes and hyperlipidemia.

No MeSH data available.


Related in: MedlinePlus