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Orientation-dependent interaction between Drosophila insulators is a property of this class of regulatory elements.

Kyrchanova O, Chetverina D, Maksimenko O, Kullyev A, Georgiev P - Nucleic Acids Res. (2008)

Bottom Line: As shown previously, Zw5, Su(Hw) and dCTCF proteins are required for the functioning of different insulators that do not interact with each other.Here, strong functional interactions have been revealed between DNA fragments containing binding sites for either Zw5 or Su(Hw) or dCTCF protein but not between heterologous binding sites [Zw5-Su(Hw), dCTCF-Su(Hw), or dCTCF-Zw5].These results suggest that insulator proteins can support selective interactions between distant regulatory elements.

View Article: PubMed Central - PubMed

Affiliation: Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.

ABSTRACT
Insulators are defined as a class of regulatory elements that delimit independent transcriptional domains within eukaryotic genomes. According to previous data, an interaction (pairing) between some Drosophila insulators can support distant activation of a promoter by an enhancer. Here, we have demonstrated that pairs of well-studied insulators such as scs-scs, scs'-scs', 1A2-1A2 and Wari-Wari support distant activation of the white promoter by the yeast GAL4 activator in an orientation-dependent manner. The same is true for the efficiency of the enhancer that stimulates white expression in the eyes. In all insulator pairs tested, stimulation of the white gene was stronger when insulators were inserted between the eye enhancer or GAL4 and the white promoter in opposite orientations relative to each other. As shown previously, Zw5, Su(Hw) and dCTCF proteins are required for the functioning of different insulators that do not interact with each other. Here, strong functional interactions have been revealed between DNA fragments containing binding sites for either Zw5 or Su(Hw) or dCTCF protein but not between heterologous binding sites [Zw5-Su(Hw), dCTCF-Su(Hw), or dCTCF-Zw5]. These results suggest that insulator proteins can support selective interactions between distant regulatory elements.

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Related in: MedlinePlus

Testing the functional interaction between (A and B) two scs’ or (C and D) two 1A2 insulators. The scs’ insulator is shown as a gray box with the black and white arrows indicating binding sites for the BEAF protein. The 1A2 insulator is shown as a black box with white rectangles indicating Su(Hw) binding sites. For other designations, see Figures 2 and 3.
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Figure 5: Testing the functional interaction between (A and B) two scs’ or (C and D) two 1A2 insulators. The scs’ insulator is shown as a gray box with the black and white arrows indicating binding sites for the BEAF protein. The 1A2 insulator is shown as a black box with white rectangles indicating Su(Hw) binding sites. For other designations, see Figures 2 and 3.

Mentions: The scs’ insulators were inserted either in opposite orientations (Figure 5A) or in the same orientation (Figure 5B). In both cases, the scs’ insulators markedly enhanced white activation by GAL4, confirming their ability to interact with each other. Once again, the relative orientation of the scs’ insulators proved to influence the level of white stimulation by GAL4.Figure 5.


Orientation-dependent interaction between Drosophila insulators is a property of this class of regulatory elements.

Kyrchanova O, Chetverina D, Maksimenko O, Kullyev A, Georgiev P - Nucleic Acids Res. (2008)

Testing the functional interaction between (A and B) two scs’ or (C and D) two 1A2 insulators. The scs’ insulator is shown as a gray box with the black and white arrows indicating binding sites for the BEAF protein. The 1A2 insulator is shown as a black box with white rectangles indicating Su(Hw) binding sites. For other designations, see Figures 2 and 3.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2602758&req=5

Figure 5: Testing the functional interaction between (A and B) two scs’ or (C and D) two 1A2 insulators. The scs’ insulator is shown as a gray box with the black and white arrows indicating binding sites for the BEAF protein. The 1A2 insulator is shown as a black box with white rectangles indicating Su(Hw) binding sites. For other designations, see Figures 2 and 3.
Mentions: The scs’ insulators were inserted either in opposite orientations (Figure 5A) or in the same orientation (Figure 5B). In both cases, the scs’ insulators markedly enhanced white activation by GAL4, confirming their ability to interact with each other. Once again, the relative orientation of the scs’ insulators proved to influence the level of white stimulation by GAL4.Figure 5.

Bottom Line: As shown previously, Zw5, Su(Hw) and dCTCF proteins are required for the functioning of different insulators that do not interact with each other.Here, strong functional interactions have been revealed between DNA fragments containing binding sites for either Zw5 or Su(Hw) or dCTCF protein but not between heterologous binding sites [Zw5-Su(Hw), dCTCF-Su(Hw), or dCTCF-Zw5].These results suggest that insulator proteins can support selective interactions between distant regulatory elements.

View Article: PubMed Central - PubMed

Affiliation: Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.

ABSTRACT
Insulators are defined as a class of regulatory elements that delimit independent transcriptional domains within eukaryotic genomes. According to previous data, an interaction (pairing) between some Drosophila insulators can support distant activation of a promoter by an enhancer. Here, we have demonstrated that pairs of well-studied insulators such as scs-scs, scs'-scs', 1A2-1A2 and Wari-Wari support distant activation of the white promoter by the yeast GAL4 activator in an orientation-dependent manner. The same is true for the efficiency of the enhancer that stimulates white expression in the eyes. In all insulator pairs tested, stimulation of the white gene was stronger when insulators were inserted between the eye enhancer or GAL4 and the white promoter in opposite orientations relative to each other. As shown previously, Zw5, Su(Hw) and dCTCF proteins are required for the functioning of different insulators that do not interact with each other. Here, strong functional interactions have been revealed between DNA fragments containing binding sites for either Zw5 or Su(Hw) or dCTCF protein but not between heterologous binding sites [Zw5-Su(Hw), dCTCF-Su(Hw), or dCTCF-Zw5]. These results suggest that insulator proteins can support selective interactions between distant regulatory elements.

Show MeSH
Related in: MedlinePlus