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Circulating IGF-binding protein 7 (IGFBP7) levels are elevated in patients with endometriosis or undergoing diabetic hemodialysis.

Kutsukake M, Ishihara R, Momose K, Isaka K, Itokazu O, Higuma C, Matsutani T, Matsuda A, Sasajima K, Hara T, Tamura K - Reprod. Biol. Endocrinol. (2008)

Bottom Line: Decreased IGFBP7 expression has been observed in some cancers and leiomyomata.Using this new ELISA, we demonstrated that cultured uterine cells secrete IGFBP7 into the medium.There were no differences in serum IGFBP7 levels in women at different stages of the menstrual cycle.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Endocrine Pharmacology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 193-0392, Japan. kutsu@ps.toyaku.ac.jp

ABSTRACT

Background: Insulin-like growth factor-binding protein-7 (IGFBP7) is a secretory protein with a molecular mass of approximately 30 kDa. It is abundantly expressed in the uterine endometrium during the secretory phase of the menstrual cycle. Decreased IGFBP7 expression has been observed in some cancers and leiomyomata.

Methods: To determine whether serum IGFBP7 levels reflect changes in uterine IGFBP7 expression in humans during the menstrual cycle, and to examine whether serum IGFBP7 levels are altered in patients with various disorders, we developed a novel, dual-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Firstly, concentrations of IGFBP7 released into the medium were determined in cultured endometrial stromal and glandular cells. Blood samples were collected from women who had normal menstrual cycles and who had been diagnosed with endometriosis. Serum from hemodialysis patients and gastrointestinal cancers was also used to determine the IGFBP7 levels.

Results: Using this new ELISA, we demonstrated that cultured uterine cells secrete IGFBP7 into the medium. Patients with endometriosis and those with type II diabetes mellitus undergoing hemodialysis had significantly higher serum concentrations of IGFBP7 than the relevant control subjects. There were no differences in serum IGFBP7 levels in women at different stages of the menstrual cycle. Furthermore, serum IGFBP7 levels in patients with colorectal, esophageal, or endometrial cancer were not different than normal healthy subjects.

Conclusion: Our observations suggest that IGFBP7 is associated with the pathophysiology of endometriosis and diabetes mellitus, and that serum IGFBP7 levels do not reflect enhanced uterine expression of IGFBP7 mRNA during the menstrual cycle.

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Serum IGFBP7 levels in hemodialysis patients with or without type II diabetes (DM). Serum from 33 hemodialysis patients without DM (15 subjects) and with DM (18 patients) was collected. *P < 0.05 as compared to without DM (Control).
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Figure 3: Serum IGFBP7 levels in hemodialysis patients with or without type II diabetes (DM). Serum from 33 hemodialysis patients without DM (15 subjects) and with DM (18 patients) was collected. *P < 0.05 as compared to without DM (Control).

Mentions: Analysis of serum IGFBP7 levels in patients with endometriosis revealed that these patients had significantly higher average levels of IGFBP7 than control subjects (Fig. 2B). For this study, the 62 female subjects with normal menstrual cycles that were analyzed in Fig. 1 served as control subjects. Based on a previous report by López-Bermejo et al. [16] showing an association of serum IGFBP7 with insulin resistance, we examined the levels of IGFBP7 in non-diabetic and diabetic patients receiving hemodialysis. Patients with type II diabetes mellitus (DM) had significantly higher IGFBP7 levels than non-DM patients (Fig. 3). Analysis of serum IGFBP7 levels of patients with endometrial, colon or esophageal cancer revealed that IGFBP7 levels did not differ significantly from those of control subjects (Fig. 4).


Circulating IGF-binding protein 7 (IGFBP7) levels are elevated in patients with endometriosis or undergoing diabetic hemodialysis.

Kutsukake M, Ishihara R, Momose K, Isaka K, Itokazu O, Higuma C, Matsutani T, Matsuda A, Sasajima K, Hara T, Tamura K - Reprod. Biol. Endocrinol. (2008)

Serum IGFBP7 levels in hemodialysis patients with or without type II diabetes (DM). Serum from 33 hemodialysis patients without DM (15 subjects) and with DM (18 patients) was collected. *P < 0.05 as compared to without DM (Control).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2600820&req=5

Figure 3: Serum IGFBP7 levels in hemodialysis patients with or without type II diabetes (DM). Serum from 33 hemodialysis patients without DM (15 subjects) and with DM (18 patients) was collected. *P < 0.05 as compared to without DM (Control).
Mentions: Analysis of serum IGFBP7 levels in patients with endometriosis revealed that these patients had significantly higher average levels of IGFBP7 than control subjects (Fig. 2B). For this study, the 62 female subjects with normal menstrual cycles that were analyzed in Fig. 1 served as control subjects. Based on a previous report by López-Bermejo et al. [16] showing an association of serum IGFBP7 with insulin resistance, we examined the levels of IGFBP7 in non-diabetic and diabetic patients receiving hemodialysis. Patients with type II diabetes mellitus (DM) had significantly higher IGFBP7 levels than non-DM patients (Fig. 3). Analysis of serum IGFBP7 levels of patients with endometrial, colon or esophageal cancer revealed that IGFBP7 levels did not differ significantly from those of control subjects (Fig. 4).

Bottom Line: Decreased IGFBP7 expression has been observed in some cancers and leiomyomata.Using this new ELISA, we demonstrated that cultured uterine cells secrete IGFBP7 into the medium.There were no differences in serum IGFBP7 levels in women at different stages of the menstrual cycle.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Endocrine Pharmacology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 193-0392, Japan. kutsu@ps.toyaku.ac.jp

ABSTRACT

Background: Insulin-like growth factor-binding protein-7 (IGFBP7) is a secretory protein with a molecular mass of approximately 30 kDa. It is abundantly expressed in the uterine endometrium during the secretory phase of the menstrual cycle. Decreased IGFBP7 expression has been observed in some cancers and leiomyomata.

Methods: To determine whether serum IGFBP7 levels reflect changes in uterine IGFBP7 expression in humans during the menstrual cycle, and to examine whether serum IGFBP7 levels are altered in patients with various disorders, we developed a novel, dual-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Firstly, concentrations of IGFBP7 released into the medium were determined in cultured endometrial stromal and glandular cells. Blood samples were collected from women who had normal menstrual cycles and who had been diagnosed with endometriosis. Serum from hemodialysis patients and gastrointestinal cancers was also used to determine the IGFBP7 levels.

Results: Using this new ELISA, we demonstrated that cultured uterine cells secrete IGFBP7 into the medium. Patients with endometriosis and those with type II diabetes mellitus undergoing hemodialysis had significantly higher serum concentrations of IGFBP7 than the relevant control subjects. There were no differences in serum IGFBP7 levels in women at different stages of the menstrual cycle. Furthermore, serum IGFBP7 levels in patients with colorectal, esophageal, or endometrial cancer were not different than normal healthy subjects.

Conclusion: Our observations suggest that IGFBP7 is associated with the pathophysiology of endometriosis and diabetes mellitus, and that serum IGFBP7 levels do not reflect enhanced uterine expression of IGFBP7 mRNA during the menstrual cycle.

Show MeSH
Related in: MedlinePlus