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Andrea Ladd: Getting to the heart of alternative splicing control. Interview by Ruth Williams.

Ladd A - J. Cell Biol. (2008)

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Factors that control alternative splicing, such as the CELF proteins that Ladd helped to identify, are starting to be thought about in the same way as transcription factors—as key regulators of gene expression programs... Now, Ladd continues to ask and answer the questions of alternative splicing regulation in her own laboratory at the Cleveland Clinic in Ohio. …I pretty much always wanted to be a biologist... It's constantly pumping, and yet undergoing morphogenesis and differentiation at the same time. …When I was thinking about a postdoc, I was looking at other developmental biology laboratories but felt that I would just be looking at the same kind of problems in another tissue... I had no idea alternative splicing was so prevalent or so important. ” I thought it was something I could go after, because I didn't think the developmental biology community had really looked at this. …When I joined the laboratory, they had just identified a new family of splicing factors called the CELF proteins, which stands for CUG-BP and ETR-3–like factors—those were the first two members of the family to be described... But there hadn't been many studies into what happens within the same cell type over time... I found evidence to suggest that CELF protein expression changes drive the developmental transition between fetal and adult patterns of splicing in the developing heart. …That's a great question... Not just how is splicing regulated, but why is it important? So we're using a variety of whole embryo, primary cell, and embryonic explant assays to go in and manipulate the splicing factors and see what effects we're having on morphogenetic processes. …It was a combination of things... But here in Cleveland, between the Cleveland Clinic and Case Western Reserve University, there's actually a huge RNA community... It's kind of stressful... When I left Tom's laboratory, I remember he told me that being a PI is like being a parent... It's one of those things that, if you had any idea going in how much work it would be or how hard it would be or how stressful it would be, you might not want to do it... But once you're there and you're doing it, it's so rewarding, you're glad you did... I don't know about being a parent, but as a PI, I'd say that's completely true.

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Andrea Ladd
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Andrea Ladd: Getting to the heart of alternative splicing control. Interview by Ruth Williams.

Ladd A - J. Cell Biol. (2008)

Andrea Ladd
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600735&req=5

fig1: Andrea Ladd

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Factors that control alternative splicing, such as the CELF proteins that Ladd helped to identify, are starting to be thought about in the same way as transcription factors—as key regulators of gene expression programs... Now, Ladd continues to ask and answer the questions of alternative splicing regulation in her own laboratory at the Cleveland Clinic in Ohio. …I pretty much always wanted to be a biologist... It's constantly pumping, and yet undergoing morphogenesis and differentiation at the same time. …When I was thinking about a postdoc, I was looking at other developmental biology laboratories but felt that I would just be looking at the same kind of problems in another tissue... I had no idea alternative splicing was so prevalent or so important. ” I thought it was something I could go after, because I didn't think the developmental biology community had really looked at this. …When I joined the laboratory, they had just identified a new family of splicing factors called the CELF proteins, which stands for CUG-BP and ETR-3–like factors—those were the first two members of the family to be described... But there hadn't been many studies into what happens within the same cell type over time... I found evidence to suggest that CELF protein expression changes drive the developmental transition between fetal and adult patterns of splicing in the developing heart. …That's a great question... Not just how is splicing regulated, but why is it important? So we're using a variety of whole embryo, primary cell, and embryonic explant assays to go in and manipulate the splicing factors and see what effects we're having on morphogenetic processes. …It was a combination of things... But here in Cleveland, between the Cleveland Clinic and Case Western Reserve University, there's actually a huge RNA community... It's kind of stressful... When I left Tom's laboratory, I remember he told me that being a PI is like being a parent... It's one of those things that, if you had any idea going in how much work it would be or how hard it would be or how stressful it would be, you might not want to do it... But once you're there and you're doing it, it's so rewarding, you're glad you did... I don't know about being a parent, but as a PI, I'd say that's completely true.

Show MeSH
Related in: MedlinePlus