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Expression of the zinc-finger transcription factor Snail in adrenocortical carcinoma is associated with decreased survival.

Waldmann J, Feldmann G, Slater EP, Langer P, Buchholz M, Ramaswamy A, Saeger W, Rothmund M, Fendrich V - Br. J. Cancer (2008)

Bottom Line: Survival rates were significantly decreased in Snail-positive tumours compared to Snail-negative tumours: 10 out of 16 vs one out of eight patients succumbed to disease after a median follow up of 14.5 and 28.5 months, respectively (P=0.03).In conclusion, we describe for the first time that Snail is expressed in a large subset of ACCs.Furthermore, Snail expression is associated with decreased survival, advanced disease and higher risk of developing distant metastases.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Philipps-Universit├Ąt Marburg, Marburg, Germany.

ABSTRACT
In this study, we evaluate whether Snail is expressed in adrenocortical cancer (ACC) and if its expression is related to patient outcome. One of the best known functions of the zinc-finger transcription factor Snail is to induce epithelial-to-mesenchymal transition (EMT). Increasing evidence suggests that EMT plays a pivotal role in tumour progression and metastatic spread. Snail and E-cadherin expression were assessed by immunohistochemistry in 26 resected ACCs and real-time quantitative RT-PCR expression analysis was performed. Data were correlated with clinical outcome and in particular with overall patient survival. Seventeen of 26 (65%) ACC tumour samples expressed Snail when assessed by immunohistochemistry. Snail expression was neither detected in normal adrenocortical tissue, nor in benign adrenocortical adenomas. Expression levels were confirmed on the mRNA level by Real-Time-PCR. Survival rates were significantly decreased in Snail-positive tumours compared to Snail-negative tumours: 10 out of 16 vs one out of eight patients succumbed to disease after a median follow up of 14.5 and 28.5 months, respectively (P=0.03). Patients with Snail-expressing ACCs presented in advanced disease (11 out of 12 vs 6 out of 14, P=0.01) and tend to develop distant metastases more frequently than patients with negative staining (7 out of 11 vs two out of eight, P=0.19). In conclusion, we describe for the first time that Snail is expressed in a large subset of ACCs. Furthermore, Snail expression is associated with decreased survival, advanced disease and higher risk of developing distant metastases.

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HE staining and IHC staining for Snail and E-cadherin in a Snail-negative ACC. (A) HE staining showing irregular shaped cells with mitoses. (B) Cells bared any Snail expression in the IHC. (C) Consecutive section of (A) and (B) revealing E-caherin expression in some cells indicated by the dark line associated to the cellular membrane.
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fig2: HE staining and IHC staining for Snail and E-cadherin in a Snail-negative ACC. (A) HE staining showing irregular shaped cells with mitoses. (B) Cells bared any Snail expression in the IHC. (C) Consecutive section of (A) and (B) revealing E-caherin expression in some cells indicated by the dark line associated to the cellular membrane.

Mentions: Nine of 26 ACCs and one LNM were Snail negative (Figure 2B). In normal adrenocortical tissue, Snail expression could not be detected by immunohistochemistry apart from a population of single cells closed to the adrenal capsule. As expected, adjacent normal mesenchymal tissue revealed Snail expression and was used as internal positive control.


Expression of the zinc-finger transcription factor Snail in adrenocortical carcinoma is associated with decreased survival.

Waldmann J, Feldmann G, Slater EP, Langer P, Buchholz M, Ramaswamy A, Saeger W, Rothmund M, Fendrich V - Br. J. Cancer (2008)

HE staining and IHC staining for Snail and E-cadherin in a Snail-negative ACC. (A) HE staining showing irregular shaped cells with mitoses. (B) Cells bared any Snail expression in the IHC. (C) Consecutive section of (A) and (B) revealing E-caherin expression in some cells indicated by the dark line associated to the cellular membrane.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600683&req=5

fig2: HE staining and IHC staining for Snail and E-cadherin in a Snail-negative ACC. (A) HE staining showing irregular shaped cells with mitoses. (B) Cells bared any Snail expression in the IHC. (C) Consecutive section of (A) and (B) revealing E-caherin expression in some cells indicated by the dark line associated to the cellular membrane.
Mentions: Nine of 26 ACCs and one LNM were Snail negative (Figure 2B). In normal adrenocortical tissue, Snail expression could not be detected by immunohistochemistry apart from a population of single cells closed to the adrenal capsule. As expected, adjacent normal mesenchymal tissue revealed Snail expression and was used as internal positive control.

Bottom Line: Survival rates were significantly decreased in Snail-positive tumours compared to Snail-negative tumours: 10 out of 16 vs one out of eight patients succumbed to disease after a median follow up of 14.5 and 28.5 months, respectively (P=0.03).In conclusion, we describe for the first time that Snail is expressed in a large subset of ACCs.Furthermore, Snail expression is associated with decreased survival, advanced disease and higher risk of developing distant metastases.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Philipps-Universit├Ąt Marburg, Marburg, Germany.

ABSTRACT
In this study, we evaluate whether Snail is expressed in adrenocortical cancer (ACC) and if its expression is related to patient outcome. One of the best known functions of the zinc-finger transcription factor Snail is to induce epithelial-to-mesenchymal transition (EMT). Increasing evidence suggests that EMT plays a pivotal role in tumour progression and metastatic spread. Snail and E-cadherin expression were assessed by immunohistochemistry in 26 resected ACCs and real-time quantitative RT-PCR expression analysis was performed. Data were correlated with clinical outcome and in particular with overall patient survival. Seventeen of 26 (65%) ACC tumour samples expressed Snail when assessed by immunohistochemistry. Snail expression was neither detected in normal adrenocortical tissue, nor in benign adrenocortical adenomas. Expression levels were confirmed on the mRNA level by Real-Time-PCR. Survival rates were significantly decreased in Snail-positive tumours compared to Snail-negative tumours: 10 out of 16 vs one out of eight patients succumbed to disease after a median follow up of 14.5 and 28.5 months, respectively (P=0.03). Patients with Snail-expressing ACCs presented in advanced disease (11 out of 12 vs 6 out of 14, P=0.01) and tend to develop distant metastases more frequently than patients with negative staining (7 out of 11 vs two out of eight, P=0.19). In conclusion, we describe for the first time that Snail is expressed in a large subset of ACCs. Furthermore, Snail expression is associated with decreased survival, advanced disease and higher risk of developing distant metastases.

Show MeSH
Related in: MedlinePlus