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Expression of the zinc-finger transcription factor Snail in adrenocortical carcinoma is associated with decreased survival.

Waldmann J, Feldmann G, Slater EP, Langer P, Buchholz M, Ramaswamy A, Saeger W, Rothmund M, Fendrich V - Br. J. Cancer (2008)

Bottom Line: Survival rates were significantly decreased in Snail-positive tumours compared to Snail-negative tumours: 10 out of 16 vs one out of eight patients succumbed to disease after a median follow up of 14.5 and 28.5 months, respectively (P=0.03).In conclusion, we describe for the first time that Snail is expressed in a large subset of ACCs.Furthermore, Snail expression is associated with decreased survival, advanced disease and higher risk of developing distant metastases.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Philipps-Universität Marburg, Marburg, Germany.

ABSTRACT
In this study, we evaluate whether Snail is expressed in adrenocortical cancer (ACC) and if its expression is related to patient outcome. One of the best known functions of the zinc-finger transcription factor Snail is to induce epithelial-to-mesenchymal transition (EMT). Increasing evidence suggests that EMT plays a pivotal role in tumour progression and metastatic spread. Snail and E-cadherin expression were assessed by immunohistochemistry in 26 resected ACCs and real-time quantitative RT-PCR expression analysis was performed. Data were correlated with clinical outcome and in particular with overall patient survival. Seventeen of 26 (65%) ACC tumour samples expressed Snail when assessed by immunohistochemistry. Snail expression was neither detected in normal adrenocortical tissue, nor in benign adrenocortical adenomas. Expression levels were confirmed on the mRNA level by Real-Time-PCR. Survival rates were significantly decreased in Snail-positive tumours compared to Snail-negative tumours: 10 out of 16 vs one out of eight patients succumbed to disease after a median follow up of 14.5 and 28.5 months, respectively (P=0.03). Patients with Snail-expressing ACCs presented in advanced disease (11 out of 12 vs 6 out of 14, P=0.01) and tend to develop distant metastases more frequently than patients with negative staining (7 out of 11 vs two out of eight, P=0.19). In conclusion, we describe for the first time that Snail is expressed in a large subset of ACCs. Furthermore, Snail expression is associated with decreased survival, advanced disease and higher risk of developing distant metastases.

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HE staining and IHC staining for Snail and E-cadherin in a human ACC ( × 10). (A) HE staining with tumour invading the surrounding fatty tissue. (B) Consecutive section with IHC staining for Snail showing Snail-expressing cells invading the fatty tissue. (C) Same specimen demonstrating the invasive front at a higher magnification ( × 40).
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fig1: HE staining and IHC staining for Snail and E-cadherin in a human ACC ( × 10). (A) HE staining with tumour invading the surrounding fatty tissue. (B) Consecutive section with IHC staining for Snail showing Snail-expressing cells invading the fatty tissue. (C) Same specimen demonstrating the invasive front at a higher magnification ( × 40).

Mentions: Overall, 17 of 26 (65%) ACC samples revealed positive immunohistochemical staining for Snail. Snail expression was inhomogeneously distributed in all positive tumour samples, revealing focal high expression close to the tumour's capsule in 8 out of 17 ACCs (Figure 1A and B). Seven of 8 of these tumours revealed a strong expression (++) of Snail at the invasive front. As seen in Figure 1B nearly every single cell along the capsular invasion showed a positive staining for Snail.


Expression of the zinc-finger transcription factor Snail in adrenocortical carcinoma is associated with decreased survival.

Waldmann J, Feldmann G, Slater EP, Langer P, Buchholz M, Ramaswamy A, Saeger W, Rothmund M, Fendrich V - Br. J. Cancer (2008)

HE staining and IHC staining for Snail and E-cadherin in a human ACC ( × 10). (A) HE staining with tumour invading the surrounding fatty tissue. (B) Consecutive section with IHC staining for Snail showing Snail-expressing cells invading the fatty tissue. (C) Same specimen demonstrating the invasive front at a higher magnification ( × 40).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600683&req=5

fig1: HE staining and IHC staining for Snail and E-cadherin in a human ACC ( × 10). (A) HE staining with tumour invading the surrounding fatty tissue. (B) Consecutive section with IHC staining for Snail showing Snail-expressing cells invading the fatty tissue. (C) Same specimen demonstrating the invasive front at a higher magnification ( × 40).
Mentions: Overall, 17 of 26 (65%) ACC samples revealed positive immunohistochemical staining for Snail. Snail expression was inhomogeneously distributed in all positive tumour samples, revealing focal high expression close to the tumour's capsule in 8 out of 17 ACCs (Figure 1A and B). Seven of 8 of these tumours revealed a strong expression (++) of Snail at the invasive front. As seen in Figure 1B nearly every single cell along the capsular invasion showed a positive staining for Snail.

Bottom Line: Survival rates were significantly decreased in Snail-positive tumours compared to Snail-negative tumours: 10 out of 16 vs one out of eight patients succumbed to disease after a median follow up of 14.5 and 28.5 months, respectively (P=0.03).In conclusion, we describe for the first time that Snail is expressed in a large subset of ACCs.Furthermore, Snail expression is associated with decreased survival, advanced disease and higher risk of developing distant metastases.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Philipps-Universität Marburg, Marburg, Germany.

ABSTRACT
In this study, we evaluate whether Snail is expressed in adrenocortical cancer (ACC) and if its expression is related to patient outcome. One of the best known functions of the zinc-finger transcription factor Snail is to induce epithelial-to-mesenchymal transition (EMT). Increasing evidence suggests that EMT plays a pivotal role in tumour progression and metastatic spread. Snail and E-cadherin expression were assessed by immunohistochemistry in 26 resected ACCs and real-time quantitative RT-PCR expression analysis was performed. Data were correlated with clinical outcome and in particular with overall patient survival. Seventeen of 26 (65%) ACC tumour samples expressed Snail when assessed by immunohistochemistry. Snail expression was neither detected in normal adrenocortical tissue, nor in benign adrenocortical adenomas. Expression levels were confirmed on the mRNA level by Real-Time-PCR. Survival rates were significantly decreased in Snail-positive tumours compared to Snail-negative tumours: 10 out of 16 vs one out of eight patients succumbed to disease after a median follow up of 14.5 and 28.5 months, respectively (P=0.03). Patients with Snail-expressing ACCs presented in advanced disease (11 out of 12 vs 6 out of 14, P=0.01) and tend to develop distant metastases more frequently than patients with negative staining (7 out of 11 vs two out of eight, P=0.19). In conclusion, we describe for the first time that Snail is expressed in a large subset of ACCs. Furthermore, Snail expression is associated with decreased survival, advanced disease and higher risk of developing distant metastases.

Show MeSH
Related in: MedlinePlus