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Sandfly fever Sicilian virus, Algeria.

Izri A, Temmam S, Moureau G, Hamrioui B, de Lamballerie X, Charrel RN - Emerging Infect. Dis. (2008)

Bottom Line: A sequence closely related to that of SFSV was detected in a Phlebotomus ariasi sandfly.Of 60 human serum samples, 3 contained immunoglobulin G against SFSV.These data suggest SFSV is present in Algeria.

View Article: PubMed Central - PubMed

Affiliation: Université Paris 13, Bobigny, France.

ABSTRACT
To determine whether sandfly fever Sicilian virus (SFSV) is present in Algeria, we tested sandflies for phlebovirus RNA. A sequence closely related to that of SFSV was detected in a Phlebotomus ariasi sandfly. Of 60 human serum samples, 3 contained immunoglobulin G against SFSV. These data suggest SFSV is present in Algeria.

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Phylogenetic analysis of Sandfly fever Sicilian virus, Algeria, based on A) 207-nt or B) 67-aa sequence in the polymerase gene. Distances and groupings were determined by the pairwise or Kimura 2-parameter algorithm and neighbor-joining method with the MEGA v2 software program (www.megasoftware.net). Bootstrap values are indicated and correspond to 500 replications. Boldface indicates virus names that correspond to sequences determined in this study. Scale bars indicate pairwise nucleotide distances (0.1 = 10%) and Kimura 2-parameter amino acid distances (0.05 = 5%).
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Figure 2: Phylogenetic analysis of Sandfly fever Sicilian virus, Algeria, based on A) 207-nt or B) 67-aa sequence in the polymerase gene. Distances and groupings were determined by the pairwise or Kimura 2-parameter algorithm and neighbor-joining method with the MEGA v2 software program (www.megasoftware.net). Bootstrap values are indicated and correspond to 500 replications. Boldface indicates virus names that correspond to sequences determined in this study. Scale bars indicate pairwise nucleotide distances (0.1 = 10%) and Kimura 2-parameter amino acid distances (0.05 = 5%).

Mentions: Together with homologous sequences of selected phleboviruses, the 3 sequences determined in this study were used to perform genetic distance comparison and phylogenetic analysis. Nucleotide and amino acid distances are presented in the Table. Distance analysis unambiguously indicated that Algeria virus is a variant genotype of SFSV. The same conclusion applied to Cyprus phlebovirus. These 3 viruses exhibited amino acid and nucleotide distances of <9.3% and <7.5%, respectively. Phylogenetic analyses (Figure 2) indicated that Algeria formed a strong cluster (100% bootstrap support) with SFSV strain Sabin and the Cyprus phlebovirus. Therefore, we propose that they can be considered as 3 variant strains of the tentative species SFSV. Because sandfly material was stored in a chaotropic solution, virus isolation was not possible, which will necessitate field work with storage conditions suitable for virus isolation attempts.


Sandfly fever Sicilian virus, Algeria.

Izri A, Temmam S, Moureau G, Hamrioui B, de Lamballerie X, Charrel RN - Emerging Infect. Dis. (2008)

Phylogenetic analysis of Sandfly fever Sicilian virus, Algeria, based on A) 207-nt or B) 67-aa sequence in the polymerase gene. Distances and groupings were determined by the pairwise or Kimura 2-parameter algorithm and neighbor-joining method with the MEGA v2 software program (www.megasoftware.net). Bootstrap values are indicated and correspond to 500 replications. Boldface indicates virus names that correspond to sequences determined in this study. Scale bars indicate pairwise nucleotide distances (0.1 = 10%) and Kimura 2-parameter amino acid distances (0.05 = 5%).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600228&req=5

Figure 2: Phylogenetic analysis of Sandfly fever Sicilian virus, Algeria, based on A) 207-nt or B) 67-aa sequence in the polymerase gene. Distances and groupings were determined by the pairwise or Kimura 2-parameter algorithm and neighbor-joining method with the MEGA v2 software program (www.megasoftware.net). Bootstrap values are indicated and correspond to 500 replications. Boldface indicates virus names that correspond to sequences determined in this study. Scale bars indicate pairwise nucleotide distances (0.1 = 10%) and Kimura 2-parameter amino acid distances (0.05 = 5%).
Mentions: Together with homologous sequences of selected phleboviruses, the 3 sequences determined in this study were used to perform genetic distance comparison and phylogenetic analysis. Nucleotide and amino acid distances are presented in the Table. Distance analysis unambiguously indicated that Algeria virus is a variant genotype of SFSV. The same conclusion applied to Cyprus phlebovirus. These 3 viruses exhibited amino acid and nucleotide distances of <9.3% and <7.5%, respectively. Phylogenetic analyses (Figure 2) indicated that Algeria formed a strong cluster (100% bootstrap support) with SFSV strain Sabin and the Cyprus phlebovirus. Therefore, we propose that they can be considered as 3 variant strains of the tentative species SFSV. Because sandfly material was stored in a chaotropic solution, virus isolation was not possible, which will necessitate field work with storage conditions suitable for virus isolation attempts.

Bottom Line: A sequence closely related to that of SFSV was detected in a Phlebotomus ariasi sandfly.Of 60 human serum samples, 3 contained immunoglobulin G against SFSV.These data suggest SFSV is present in Algeria.

View Article: PubMed Central - PubMed

Affiliation: Université Paris 13, Bobigny, France.

ABSTRACT
To determine whether sandfly fever Sicilian virus (SFSV) is present in Algeria, we tested sandflies for phlebovirus RNA. A sequence closely related to that of SFSV was detected in a Phlebotomus ariasi sandfly. Of 60 human serum samples, 3 contained immunoglobulin G against SFSV. These data suggest SFSV is present in Algeria.

Show MeSH
Related in: MedlinePlus