Limits...
Unique Cryptosporidium population in HIV-infected persons, Jamaica.

Gatei W, Barrett D, Lindo JF, Eldemire-Shearer D, Cama V, Xiao L - Emerging Infect. Dis. (2008)

Bottom Line: A cryptosporidiosis survey showed the presence of Cryptosporidium hominis, C. parvum, C. canis, and C. felis in 25, 7, 1, and 1 HIV-positive persons from Jamaica, respectively; 1 person had both C. hominis and C. felis.Multilocus sequence typing indicated the presence of a homogeneous but geographically distinct C. hominis population in Jamaica.

View Article: PubMed Central - PubMed

Affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA.

ABSTRACT
A cryptosporidiosis survey showed the presence of Cryptosporidium hominis, C. parvum, C. canis, and C. felis in 25, 7, 1, and 1 HIV-positive persons from Jamaica, respectively; 1 person had both C. hominis and C. felis. Multilocus sequence typing indicated the presence of a homogeneous but geographically distinct C. hominis population in Jamaica.

Show MeSH

Related in: MedlinePlus

Relationships among Cryptosporidium hominis and C. parvum multilocus sequence subtypes at 5 genetic loci. Parasite population from Jamaica was compared with that from other regions by neighbor-joining analysis of concatenated sequence of 5 genetic loci, by using GP60 subtype identification in specimen selection. The Kimura 2-parameter model was used in the distance calculations. The sequences reported in this paper are available in the GenBank database under accession nos. EU141710–EU141727.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2600223&req=5

Figure 1: Relationships among Cryptosporidium hominis and C. parvum multilocus sequence subtypes at 5 genetic loci. Parasite population from Jamaica was compared with that from other regions by neighbor-joining analysis of concatenated sequence of 5 genetic loci, by using GP60 subtype identification in specimen selection. The Kimura 2-parameter model was used in the distance calculations. The sequences reported in this paper are available in the GenBank database under accession nos. EU141710–EU141727.

Mentions: To measure the degree of heterogeneity of C. hominis and C. parvum, an MLST analysis was conducted to compare MLST types from Jamaica with those from other geographic areas of similar GP60 subtype families Ib, Ie and IIc (Kenya, Peru, India, and USA). All sequences for each specimen at the 5 loci were concatenated and multilocus subtypes generated. The analysis showed C. hominis multilocus subtypes from Jamaica clustered in 1 group, regardless of their GP60 subtype designation. Specimens of the same GP60 subtypes from other regions clustered in clades separate from the Jamaica specimens, forming largely distinct monophyletic groups defined by geographic origin. Specimens from Kenya and India clustered in 1 clade because of extensive human migration between the 2 countries. Although all C. parvum specimens from Jamaica were identified as IIcA5G3d, 4 MLST types were present, highlighting the extent of genetic diversity of C. parvum in this study. The relationship of the multilocus subtypes based on the 5 loci inferred by neighbor-joining analysis is shown in the Figure.


Unique Cryptosporidium population in HIV-infected persons, Jamaica.

Gatei W, Barrett D, Lindo JF, Eldemire-Shearer D, Cama V, Xiao L - Emerging Infect. Dis. (2008)

Relationships among Cryptosporidium hominis and C. parvum multilocus sequence subtypes at 5 genetic loci. Parasite population from Jamaica was compared with that from other regions by neighbor-joining analysis of concatenated sequence of 5 genetic loci, by using GP60 subtype identification in specimen selection. The Kimura 2-parameter model was used in the distance calculations. The sequences reported in this paper are available in the GenBank database under accession nos. EU141710–EU141727.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600223&req=5

Figure 1: Relationships among Cryptosporidium hominis and C. parvum multilocus sequence subtypes at 5 genetic loci. Parasite population from Jamaica was compared with that from other regions by neighbor-joining analysis of concatenated sequence of 5 genetic loci, by using GP60 subtype identification in specimen selection. The Kimura 2-parameter model was used in the distance calculations. The sequences reported in this paper are available in the GenBank database under accession nos. EU141710–EU141727.
Mentions: To measure the degree of heterogeneity of C. hominis and C. parvum, an MLST analysis was conducted to compare MLST types from Jamaica with those from other geographic areas of similar GP60 subtype families Ib, Ie and IIc (Kenya, Peru, India, and USA). All sequences for each specimen at the 5 loci were concatenated and multilocus subtypes generated. The analysis showed C. hominis multilocus subtypes from Jamaica clustered in 1 group, regardless of their GP60 subtype designation. Specimens of the same GP60 subtypes from other regions clustered in clades separate from the Jamaica specimens, forming largely distinct monophyletic groups defined by geographic origin. Specimens from Kenya and India clustered in 1 clade because of extensive human migration between the 2 countries. Although all C. parvum specimens from Jamaica were identified as IIcA5G3d, 4 MLST types were present, highlighting the extent of genetic diversity of C. parvum in this study. The relationship of the multilocus subtypes based on the 5 loci inferred by neighbor-joining analysis is shown in the Figure.

Bottom Line: A cryptosporidiosis survey showed the presence of Cryptosporidium hominis, C. parvum, C. canis, and C. felis in 25, 7, 1, and 1 HIV-positive persons from Jamaica, respectively; 1 person had both C. hominis and C. felis.Multilocus sequence typing indicated the presence of a homogeneous but geographically distinct C. hominis population in Jamaica.

View Article: PubMed Central - PubMed

Affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA.

ABSTRACT
A cryptosporidiosis survey showed the presence of Cryptosporidium hominis, C. parvum, C. canis, and C. felis in 25, 7, 1, and 1 HIV-positive persons from Jamaica, respectively; 1 person had both C. hominis and C. felis. Multilocus sequence typing indicated the presence of a homogeneous but geographically distinct C. hominis population in Jamaica.

Show MeSH
Related in: MedlinePlus