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Saksenaea vasiformis infection, French Guiana.

Blanchet D, Dannaoui E, Fior A, Huber F, Couppié P, Salhab N, Hoinard D, Aznar C - Emerging Infect. Dis. (2008)

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We report a case of a cutaneous lesion caused by S. vasiformis in French Guiana... Microscopy examination showed extensive superficial mycotic proliferation, with wide and irregular ribbonlike nonseptate hyphae and right-angle branching... These hyphae extended toward the hypodermic fat tissues and were associated with a break in the cell membrane of adipocytes and with crystals inside the adipocytes... These lesions were associated with massive nonsuppurative vascular thrombosis... Sporulation also occurred on Czapek agar after 7 days’ incubation at 37°C... Sporulation on Czapek agar enabled preparation of a sporangiospore suspension used for antifungal susceptibility testing... MICs, determined by the EUCAST reference microdilution method, after 48 h of incubation were >8, 2, >8, 0.5, and >8 µg/mL for amphotericin B, itraconazole, voriconazole, posaconazole, and caspofungin, respectively... This fungus has been rarely responsible for human infections... A recent review, which did not include infrequently cited articles, found only 30 human cases... This scarcity may occur because the diagnosis is often based on histologic features and S. vasiformis does not sporulate in routine mycology media... Rare Zygomycetes species such as S. vasiformis or Apophysomyces elegans should be suspected when a nonsporulating zygomycete is isolated from an infected lesion... When this acute infection is suspected after examination of tissue by microscopy, the fungi should be cultured in specific culture media to induce sporulation or they should be identified by molecular tools.

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Microscopic characteristics of the isolate of Saksenaea vasiformis cultured on Czapek agar. A) Typical flask-shaped sporangia (scale bar = 25 μm) containing B) smooth-walled, rectangular sporangiospores (scale bar = 10 μm).
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Figure 1: Microscopic characteristics of the isolate of Saksenaea vasiformis cultured on Czapek agar. A) Typical flask-shaped sporangia (scale bar = 25 μm) containing B) smooth-walled, rectangular sporangiospores (scale bar = 10 μm).

Mentions: Culture of tissues samples on Sabouraud-chloramphenicol-gentamicin agar after 4 days at 30°C and 37°C grew a white aerial mold, which covered the entire surface of the agar. Examination by microscopy showed nonseptate sterile hyphae typical of a zygomycete. The fungal isolate was sent to the National Reference Center for Mycology and Antifungals at the Institut Pasteur, Paris. Subcultures on different media including malt extract agar and potato dextrose agar grew sterile mycelia. The isolates were then cultured in nutritionally deficient medium consisting of sterile distilled water supplemented with 0.05% filter-sterilized yeast extract (Difco, Becton, Dickinson and Company, Sparks, MD, USA) solution for 7 days at 37°C (2). Typical flask-shaped sporangia enabled identification of S. vasiformis (Figure). Sporulation also occurred on Czapek agar after 7 days’ incubation at 37°C.


Saksenaea vasiformis infection, French Guiana.

Blanchet D, Dannaoui E, Fior A, Huber F, Couppié P, Salhab N, Hoinard D, Aznar C - Emerging Infect. Dis. (2008)

Microscopic characteristics of the isolate of Saksenaea vasiformis cultured on Czapek agar. A) Typical flask-shaped sporangia (scale bar = 25 μm) containing B) smooth-walled, rectangular sporangiospores (scale bar = 10 μm).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600207&req=5

Figure 1: Microscopic characteristics of the isolate of Saksenaea vasiformis cultured on Czapek agar. A) Typical flask-shaped sporangia (scale bar = 25 μm) containing B) smooth-walled, rectangular sporangiospores (scale bar = 10 μm).
Mentions: Culture of tissues samples on Sabouraud-chloramphenicol-gentamicin agar after 4 days at 30°C and 37°C grew a white aerial mold, which covered the entire surface of the agar. Examination by microscopy showed nonseptate sterile hyphae typical of a zygomycete. The fungal isolate was sent to the National Reference Center for Mycology and Antifungals at the Institut Pasteur, Paris. Subcultures on different media including malt extract agar and potato dextrose agar grew sterile mycelia. The isolates were then cultured in nutritionally deficient medium consisting of sterile distilled water supplemented with 0.05% filter-sterilized yeast extract (Difco, Becton, Dickinson and Company, Sparks, MD, USA) solution for 7 days at 37°C (2). Typical flask-shaped sporangia enabled identification of S. vasiformis (Figure). Sporulation also occurred on Czapek agar after 7 days’ incubation at 37°C.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

We report a case of a cutaneous lesion caused by S. vasiformis in French Guiana... Microscopy examination showed extensive superficial mycotic proliferation, with wide and irregular ribbonlike nonseptate hyphae and right-angle branching... These hyphae extended toward the hypodermic fat tissues and were associated with a break in the cell membrane of adipocytes and with crystals inside the adipocytes... These lesions were associated with massive nonsuppurative vascular thrombosis... Sporulation also occurred on Czapek agar after 7 days’ incubation at 37°C... Sporulation on Czapek agar enabled preparation of a sporangiospore suspension used for antifungal susceptibility testing... MICs, determined by the EUCAST reference microdilution method, after 48 h of incubation were >8, 2, >8, 0.5, and >8 µg/mL for amphotericin B, itraconazole, voriconazole, posaconazole, and caspofungin, respectively... This fungus has been rarely responsible for human infections... A recent review, which did not include infrequently cited articles, found only 30 human cases... This scarcity may occur because the diagnosis is often based on histologic features and S. vasiformis does not sporulate in routine mycology media... Rare Zygomycetes species such as S. vasiformis or Apophysomyces elegans should be suspected when a nonsporulating zygomycete is isolated from an infected lesion... When this acute infection is suspected after examination of tissue by microscopy, the fungi should be cultured in specific culture media to induce sporulation or they should be identified by molecular tools.

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