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Methicillin-resistant Staphylococcus aureus, Geneva, Switzerland, 1993-2005.

Francois P, Harbarth S, Huyghe A, Renzi G, Bento M, Gervaix A, Pittet D, Schrenzel J - Emerging Infect. Dis. (2008)

Bottom Line: Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) strains different from those of an endemic healthcare-associated clone was conducted over 13 years in Geneva, Switzerland.We demonstrated strain diversity, including clones rarely found in Europe.Local epidemiology of community-associated MRSA is diverse and is evolving by importation and transmission of new strains.

View Article: PubMed Central - PubMed

Affiliation: University of Geneva Hospitals, Geneva, Switzerland. patrice.francois@genomic.ch

ABSTRACT
Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) strains different from those of an endemic healthcare-associated clone was conducted over 13 years in Geneva, Switzerland. We demonstrated strain diversity, including clones rarely found in Europe. Local epidemiology of community-associated MRSA is diverse and is evolving by importation and transmission of new strains.

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Incidence of non–multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA) strains, Geneva, Switzerland, 1993–2005. A) Number of strains collected since 1993 showing an atypical multidrug-susceptible phenotype (white bars). Also shown are the number of SCCmec IV and V (circles) isolates and number of strains containing Panton-Valentine leukocidin (PVL) (triangles). B) Evolution of the 3 most abundant clonotypes (ST80, ST88, and ST5). Despite a constant number of strains isolated since 2002, the proportion of these clones has decreased, which suggests increasing diversity of clones in our population of community acquired–MRSA.
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Figure 1: Incidence of non–multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA) strains, Geneva, Switzerland, 1993–2005. A) Number of strains collected since 1993 showing an atypical multidrug-susceptible phenotype (white bars). Also shown are the number of SCCmec IV and V (circles) isolates and number of strains containing Panton-Valentine leukocidin (PVL) (triangles). B) Evolution of the 3 most abundant clonotypes (ST80, ST88, and ST5). Despite a constant number of strains isolated since 2002, the proportion of these clones has decreased, which suggests increasing diversity of clones in our population of community acquired–MRSA.

Mentions: The Figure, panel A, shows the incidence of isolates fulfilling our entry criteria and the proportion of strains producing PVL or harboring SCCmec IV or V. An increase in non–multidrug-resistant MRSA was observed during 1994–1997 (incidence 2.3 cases/10,000 admissions in 1997), and a second peak was observed during 2002–2005 (incidence 4.3 cases/10,000 admissions in 2004). Molecular characterization of the 151 strains showed that 124 (82%) harbored either SCCmec IV or V. A total of 92 isolates (61%) harbored at least 1 toxin gene, most frequently PVL (n = 60), followed by TSST-1 (n = 22) and exfoliatin A (n = 11). An isolate (ST149-MRSA-IV) from a Libyan patient harbored the PVL and TSST-1 genes (Table 2). A strain with PVL (ST80-MRSA-IV) was isolated in 1994 from a 73-year-old man from Libya. A case of bacteremia with PVL-positive CA-MRSA (ST80-MRSA-IV) was documented in a 28-year-old Tunisian woman who had an abscess of her left forearm in 2000. No case of necrotizing pneumonia was observed.


Methicillin-resistant Staphylococcus aureus, Geneva, Switzerland, 1993-2005.

Francois P, Harbarth S, Huyghe A, Renzi G, Bento M, Gervaix A, Pittet D, Schrenzel J - Emerging Infect. Dis. (2008)

Incidence of non–multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA) strains, Geneva, Switzerland, 1993–2005. A) Number of strains collected since 1993 showing an atypical multidrug-susceptible phenotype (white bars). Also shown are the number of SCCmec IV and V (circles) isolates and number of strains containing Panton-Valentine leukocidin (PVL) (triangles). B) Evolution of the 3 most abundant clonotypes (ST80, ST88, and ST5). Despite a constant number of strains isolated since 2002, the proportion of these clones has decreased, which suggests increasing diversity of clones in our population of community acquired–MRSA.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600191&req=5

Figure 1: Incidence of non–multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA) strains, Geneva, Switzerland, 1993–2005. A) Number of strains collected since 1993 showing an atypical multidrug-susceptible phenotype (white bars). Also shown are the number of SCCmec IV and V (circles) isolates and number of strains containing Panton-Valentine leukocidin (PVL) (triangles). B) Evolution of the 3 most abundant clonotypes (ST80, ST88, and ST5). Despite a constant number of strains isolated since 2002, the proportion of these clones has decreased, which suggests increasing diversity of clones in our population of community acquired–MRSA.
Mentions: The Figure, panel A, shows the incidence of isolates fulfilling our entry criteria and the proportion of strains producing PVL or harboring SCCmec IV or V. An increase in non–multidrug-resistant MRSA was observed during 1994–1997 (incidence 2.3 cases/10,000 admissions in 1997), and a second peak was observed during 2002–2005 (incidence 4.3 cases/10,000 admissions in 2004). Molecular characterization of the 151 strains showed that 124 (82%) harbored either SCCmec IV or V. A total of 92 isolates (61%) harbored at least 1 toxin gene, most frequently PVL (n = 60), followed by TSST-1 (n = 22) and exfoliatin A (n = 11). An isolate (ST149-MRSA-IV) from a Libyan patient harbored the PVL and TSST-1 genes (Table 2). A strain with PVL (ST80-MRSA-IV) was isolated in 1994 from a 73-year-old man from Libya. A case of bacteremia with PVL-positive CA-MRSA (ST80-MRSA-IV) was documented in a 28-year-old Tunisian woman who had an abscess of her left forearm in 2000. No case of necrotizing pneumonia was observed.

Bottom Line: Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) strains different from those of an endemic healthcare-associated clone was conducted over 13 years in Geneva, Switzerland.We demonstrated strain diversity, including clones rarely found in Europe.Local epidemiology of community-associated MRSA is diverse and is evolving by importation and transmission of new strains.

View Article: PubMed Central - PubMed

Affiliation: University of Geneva Hospitals, Geneva, Switzerland. patrice.francois@genomic.ch

ABSTRACT
Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) strains different from those of an endemic healthcare-associated clone was conducted over 13 years in Geneva, Switzerland. We demonstrated strain diversity, including clones rarely found in Europe. Local epidemiology of community-associated MRSA is diverse and is evolving by importation and transmission of new strains.

Show MeSH
Related in: MedlinePlus