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Dengue virus 3 genotype 1 associated with dengue fever and dengue hemorrhagic fever, Brazil.

Barcelos Figueiredo L, Batista Cecílio A, Portela Ferreira G, Paiva Drumond B, Germano de Oliveira J, Bonjardim CA, Peregrino Ferreira PC, Kroon EG - Emerging Infect. Dis. (2008)

Bottom Line: Dengue serotype 3 viruses were isolated from patients in Brazil from 2002 through 2004.On the basis of phylogenetic analyses, these isolates were assigned genotype 1.Its appearance indicates a major risk factor for dengue epidemics and severe disease.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Dengue serotype 3 viruses were isolated from patients in Brazil from 2002 through 2004. On the basis of phylogenetic analyses, these isolates were assigned genotype 1. This genotype had never been reported in South America before. Its appearance indicates a major risk factor for dengue epidemics and severe disease.

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Related in: MedlinePlus

Phylogenetic trees of established dengue virus serotype 3 (DENV-3) and new sequences from Minas Gerais State, Brazil, identified in this study. A) The tree is based on a 504-nt sequence alignment comprising the C-prM gene (nucleotides 137–638). B) The tree is based on a 1,023-nt partial E nucleotide sequences (nucleotides 1022–2008). This tree was generated by neighbor-joining using the Tamura Nei model implemented by using MEGA3 software (www.megasoftware.net). Numbers to the left of nodes represent bootstrap values (1,000 replicates) in support of grouping to the right. Numbers to the right in parentheses of branches indicate the GenBank accession number. Roman numerals denote the different genotypes of DENV-3.
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Figure 2: Phylogenetic trees of established dengue virus serotype 3 (DENV-3) and new sequences from Minas Gerais State, Brazil, identified in this study. A) The tree is based on a 504-nt sequence alignment comprising the C-prM gene (nucleotides 137–638). B) The tree is based on a 1,023-nt partial E nucleotide sequences (nucleotides 1022–2008). This tree was generated by neighbor-joining using the Tamura Nei model implemented by using MEGA3 software (www.megasoftware.net). Numbers to the left of nodes represent bootstrap values (1,000 replicates) in support of grouping to the right. Numbers to the right in parentheses of branches indicate the GenBank accession number. Roman numerals denote the different genotypes of DENV-3.

Mentions: Sequence comparisons showed high degrees of identity among our isolates, and the paired identity at the nucleotide level ranged from 99.2% to 100% and from 99.7% to 99.9% regarding the C-prM region and the envelope gene, respectively. When sequences were compared with genotype 3 isolates, including isolates from Latin America, the Indian subcontinent, and East Africa, the identity values ranged from 95.4% to 96.2% and from 94.0 to 95.6% in relation to the C-prM and envelope genes, respectively. When those were compared to genotype 1 sequences from the Philippines and China, the nucleotide identities of C-prM region ranged from 98.4% to 99.4%; when envelope sequences were analyzed, values from 99.1% to 99.5% nucleotide identity were observed. According to phylogenetic clustering with other DENV strains (Figure 2), viruses were classified into a specific serotype and genotype. Both phylogenetic trees showed isolates from Brazil grouped together in a well-supported distinct cluster of genotype 1 isolates.


Dengue virus 3 genotype 1 associated with dengue fever and dengue hemorrhagic fever, Brazil.

Barcelos Figueiredo L, Batista Cecílio A, Portela Ferreira G, Paiva Drumond B, Germano de Oliveira J, Bonjardim CA, Peregrino Ferreira PC, Kroon EG - Emerging Infect. Dis. (2008)

Phylogenetic trees of established dengue virus serotype 3 (DENV-3) and new sequences from Minas Gerais State, Brazil, identified in this study. A) The tree is based on a 504-nt sequence alignment comprising the C-prM gene (nucleotides 137–638). B) The tree is based on a 1,023-nt partial E nucleotide sequences (nucleotides 1022–2008). This tree was generated by neighbor-joining using the Tamura Nei model implemented by using MEGA3 software (www.megasoftware.net). Numbers to the left of nodes represent bootstrap values (1,000 replicates) in support of grouping to the right. Numbers to the right in parentheses of branches indicate the GenBank accession number. Roman numerals denote the different genotypes of DENV-3.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600180&req=5

Figure 2: Phylogenetic trees of established dengue virus serotype 3 (DENV-3) and new sequences from Minas Gerais State, Brazil, identified in this study. A) The tree is based on a 504-nt sequence alignment comprising the C-prM gene (nucleotides 137–638). B) The tree is based on a 1,023-nt partial E nucleotide sequences (nucleotides 1022–2008). This tree was generated by neighbor-joining using the Tamura Nei model implemented by using MEGA3 software (www.megasoftware.net). Numbers to the left of nodes represent bootstrap values (1,000 replicates) in support of grouping to the right. Numbers to the right in parentheses of branches indicate the GenBank accession number. Roman numerals denote the different genotypes of DENV-3.
Mentions: Sequence comparisons showed high degrees of identity among our isolates, and the paired identity at the nucleotide level ranged from 99.2% to 100% and from 99.7% to 99.9% regarding the C-prM region and the envelope gene, respectively. When sequences were compared with genotype 3 isolates, including isolates from Latin America, the Indian subcontinent, and East Africa, the identity values ranged from 95.4% to 96.2% and from 94.0 to 95.6% in relation to the C-prM and envelope genes, respectively. When those were compared to genotype 1 sequences from the Philippines and China, the nucleotide identities of C-prM region ranged from 98.4% to 99.4%; when envelope sequences were analyzed, values from 99.1% to 99.5% nucleotide identity were observed. According to phylogenetic clustering with other DENV strains (Figure 2), viruses were classified into a specific serotype and genotype. Both phylogenetic trees showed isolates from Brazil grouped together in a well-supported distinct cluster of genotype 1 isolates.

Bottom Line: Dengue serotype 3 viruses were isolated from patients in Brazil from 2002 through 2004.On the basis of phylogenetic analyses, these isolates were assigned genotype 1.Its appearance indicates a major risk factor for dengue epidemics and severe disease.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Dengue serotype 3 viruses were isolated from patients in Brazil from 2002 through 2004. On the basis of phylogenetic analyses, these isolates were assigned genotype 1. This genotype had never been reported in South America before. Its appearance indicates a major risk factor for dengue epidemics and severe disease.

Show MeSH
Related in: MedlinePlus