Limits...
Unexpected occurrence of plasmid-mediated quinolone resistance determinants in environmental Aeromonas spp.

Cattoir V, Poirel L, Aubert C, Soussy CJ, Nordmann P - Emerging Infect. Dis. (2008)

Bottom Line: The qnrS2 genes were identified from Aeromonas punctata subsp. punctata and A. media.The qnrS2 gene was located on IncU-type plasmids in both isolates, which resulted in increased MIC values of quinolones and fluoroquinolones, once they were transferred into Escherichia coli.This identification of plasmid-mediated qnr genes outside Enterobacteriaceae underlines a possible diffusion of those resistance determinants within gram-negative rods.

View Article: PubMed Central - PubMed

Affiliation: Institut Nationale de la Santé et de la Recherche Médicale Unité 914, Le Kremlin-Bicêtre, France.

ABSTRACT
We searched for plasmid-mediated quinolone resistance determinants of the Qnr type in several water samples collected at diverse locations from the Seine River (Paris, France). The qnrS2 genes were identified from Aeromonas punctata subsp. punctata and A. media. The qnrS2 gene was located on IncU-type plasmids in both isolates, which resulted in increased MIC values of quinolones and fluoroquinolones, once they were transferred into Escherichia coli. The qnrS2 gene identified in A. punctata was part of novel genetic structure corresponding to a mobile insertion cassette element. This identification of plasmid-mediated qnr genes outside Enterobacteriaceae underlines a possible diffusion of those resistance determinants within gram-negative rods.

Show MeSH

Related in: MedlinePlus

Plasmid DNAs from Aeromonas punctata 37 and A. media 42 and their Escherichia coli TOP10 transformants (TF) carrying plasmids p37 or p42. Lanes: 1, A. punctata 37; 2, E. coli TOP10/p37 TF-1; 3, E. coli TOP10/p37 TF-2; 4, A. media 42; 5, E. coli TOP10/p42 TF-1; 6, E. coli TOP10/p42 TF-2; M, E. coli NCTC 50192 (used as reference for plasmid sizes).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2600179&req=5

Figure 1: Plasmid DNAs from Aeromonas punctata 37 and A. media 42 and their Escherichia coli TOP10 transformants (TF) carrying plasmids p37 or p42. Lanes: 1, A. punctata 37; 2, E. coli TOP10/p37 TF-1; 3, E. coli TOP10/p37 TF-2; 4, A. media 42; 5, E. coli TOP10/p42 TF-1; 6, E. coli TOP10/p42 TF-2; M, E. coli NCTC 50192 (used as reference for plasmid sizes).

Mentions: The plasmid-mediated quinolone resistance QnrS2 determinant was transferred from Aeromonas isolates 37 and 42 to E. coli TOP10 recipient strain by electrotransformation, but repeated conjugation experiments failed. Plasmid analysis identified a single 55-kb plasmid (p37) and a single 20-kb plasmid (p42) from E. coli transformants from A. punctata 37 and A. media 42 isolates, respectively (Figure 1). After they were transferred into E. coli TOP10, plasmids p37 and p42 conferred increased MICs of quinolones and fluoroquinolones (Table). No other antimicrobial resistance marker was carried by those natural plasmids.


Unexpected occurrence of plasmid-mediated quinolone resistance determinants in environmental Aeromonas spp.

Cattoir V, Poirel L, Aubert C, Soussy CJ, Nordmann P - Emerging Infect. Dis. (2008)

Plasmid DNAs from Aeromonas punctata 37 and A. media 42 and their Escherichia coli TOP10 transformants (TF) carrying plasmids p37 or p42. Lanes: 1, A. punctata 37; 2, E. coli TOP10/p37 TF-1; 3, E. coli TOP10/p37 TF-2; 4, A. media 42; 5, E. coli TOP10/p42 TF-1; 6, E. coli TOP10/p42 TF-2; M, E. coli NCTC 50192 (used as reference for plasmid sizes).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600179&req=5

Figure 1: Plasmid DNAs from Aeromonas punctata 37 and A. media 42 and their Escherichia coli TOP10 transformants (TF) carrying plasmids p37 or p42. Lanes: 1, A. punctata 37; 2, E. coli TOP10/p37 TF-1; 3, E. coli TOP10/p37 TF-2; 4, A. media 42; 5, E. coli TOP10/p42 TF-1; 6, E. coli TOP10/p42 TF-2; M, E. coli NCTC 50192 (used as reference for plasmid sizes).
Mentions: The plasmid-mediated quinolone resistance QnrS2 determinant was transferred from Aeromonas isolates 37 and 42 to E. coli TOP10 recipient strain by electrotransformation, but repeated conjugation experiments failed. Plasmid analysis identified a single 55-kb plasmid (p37) and a single 20-kb plasmid (p42) from E. coli transformants from A. punctata 37 and A. media 42 isolates, respectively (Figure 1). After they were transferred into E. coli TOP10, plasmids p37 and p42 conferred increased MICs of quinolones and fluoroquinolones (Table). No other antimicrobial resistance marker was carried by those natural plasmids.

Bottom Line: The qnrS2 genes were identified from Aeromonas punctata subsp. punctata and A. media.The qnrS2 gene was located on IncU-type plasmids in both isolates, which resulted in increased MIC values of quinolones and fluoroquinolones, once they were transferred into Escherichia coli.This identification of plasmid-mediated qnr genes outside Enterobacteriaceae underlines a possible diffusion of those resistance determinants within gram-negative rods.

View Article: PubMed Central - PubMed

Affiliation: Institut Nationale de la Santé et de la Recherche Médicale Unité 914, Le Kremlin-Bicêtre, France.

ABSTRACT
We searched for plasmid-mediated quinolone resistance determinants of the Qnr type in several water samples collected at diverse locations from the Seine River (Paris, France). The qnrS2 genes were identified from Aeromonas punctata subsp. punctata and A. media. The qnrS2 gene was located on IncU-type plasmids in both isolates, which resulted in increased MIC values of quinolones and fluoroquinolones, once they were transferred into Escherichia coli. The qnrS2 gene identified in A. punctata was part of novel genetic structure corresponding to a mobile insertion cassette element. This identification of plasmid-mediated qnr genes outside Enterobacteriaceae underlines a possible diffusion of those resistance determinants within gram-negative rods.

Show MeSH
Related in: MedlinePlus