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Human case of Streptococcus suis serotype 16 infection.

Nghia HD, Hoa NT, Linh le D, Campbell J, Diep TS, Chau NV, Mai NT, Hien TT, Spratt B, Farrar J, Schultsz C - Emerging Infect. Dis. (2008)

Bottom Line: Streptococcus suis infection is an emerging zoonosis in Southeast Asia.We report a fatal case of S. suis serotype 16 infection in a Vietnamese man in 2001.

View Article: PubMed Central - PubMed

Affiliation: Oxford University Clinical Research Unit, The Hospital for Tropical Diseases, 190 Ben Ham Tu, Quan 5, Ho Chi Minh City, Vietnam.

ABSTRACT
Streptococcus suis infection is an emerging zoonosis in Southeast Asia. We report a fatal case of S. suis serotype 16 infection in a Vietnamese man in 2001.

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Related in: MedlinePlus

Pulsed-field gel electrophoresis after SmaI digestion of Streptococcus suis serotype 16 strain SS07 and a representative set of S. suis serotype 2 strains isolated from patients with meningitis in southern Vietnam. A dendrogram was generated by Dice analysis (optimization 0.5%, band tolerance 1%) and cluster analysis with unweighted pair group method with arithmetic average, using Bionumerics software (Applied Maths, Sint-Martens-Latem, Belgium). Numbers in dendrogram indicate percentage of similarity. Arrow numbers indicate molecular size (kb).
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Figure 1: Pulsed-field gel electrophoresis after SmaI digestion of Streptococcus suis serotype 16 strain SS07 and a representative set of S. suis serotype 2 strains isolated from patients with meningitis in southern Vietnam. A dendrogram was generated by Dice analysis (optimization 0.5%, band tolerance 1%) and cluster analysis with unweighted pair group method with arithmetic average, using Bionumerics software (Applied Maths, Sint-Martens-Latem, Belgium). Numbers in dendrogram indicate percentage of similarity. Arrow numbers indicate molecular size (kb).

Mentions: S. suis was identified on the basis of colony morphology, negative katalase reaction, optochin resistance, and APIStrep (bioMérieux, Marcy l’Etoile, France). Serotyping was performed by slide agglutination using specific antisera (Statens Serum Institute, Copenhagen, Denmark) and was positive for serotype 16. Confirmation of the serotype was performed at the International Reference Laboratory for S. suis Serotyping, Quebec City, Quebec, Canada (M. Gottschalk). This strain was susceptible to penicillin (MIC 0.032 mg/L), ceftriaxone (0.064 mg/L), rifampin (0.032 mg/L), chloramphenicol (2 mg/L), erythromycin (0.064 mg/L), levofloxacin (0.38 mg/L), and vancomycin (0.5 mg/L) but resistant to tetracycline (64 mg/L) by Etest (AB-Biodisk, Solna, Sweden) when Clinical Laboratory Standard Institute breakpoints were used. On pulsed-field gel electrophoresis (PFGE) that used restriction enzyme SmaI, this strain showed little similarity with a representative set of serotype 2 isolates from Vietnam (Figure). Multilocus sequence typing (MLST) (www.mlst.net) showed that the sequence of 5 of 7 alleles of the included housekeeping genes had not been previously described. Thus, this strain was assigned the new sequence type 106. On eBURST analysis (www.mlst.net), this sequence type does not belong to any of the clonal complexes but is a singleton. PCR for detection of the genes encoding the putative virulence factors extracellular protein factor (EF) and suilysin were negative. Results of Western blot for detection of muramidase-released protein (MRP) and EF, using rabbit polyclonal antibody against MRP and EF (provided by H. Smith, the Netherlands), were also negative. S. suis serotype 2 strains 31533 and 89-1591 (provided by M. Gottschalk, Canada) were used as positive and negative controls, respectively.


Human case of Streptococcus suis serotype 16 infection.

Nghia HD, Hoa NT, Linh le D, Campbell J, Diep TS, Chau NV, Mai NT, Hien TT, Spratt B, Farrar J, Schultsz C - Emerging Infect. Dis. (2008)

Pulsed-field gel electrophoresis after SmaI digestion of Streptococcus suis serotype 16 strain SS07 and a representative set of S. suis serotype 2 strains isolated from patients with meningitis in southern Vietnam. A dendrogram was generated by Dice analysis (optimization 0.5%, band tolerance 1%) and cluster analysis with unweighted pair group method with arithmetic average, using Bionumerics software (Applied Maths, Sint-Martens-Latem, Belgium). Numbers in dendrogram indicate percentage of similarity. Arrow numbers indicate molecular size (kb).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600150&req=5

Figure 1: Pulsed-field gel electrophoresis after SmaI digestion of Streptococcus suis serotype 16 strain SS07 and a representative set of S. suis serotype 2 strains isolated from patients with meningitis in southern Vietnam. A dendrogram was generated by Dice analysis (optimization 0.5%, band tolerance 1%) and cluster analysis with unweighted pair group method with arithmetic average, using Bionumerics software (Applied Maths, Sint-Martens-Latem, Belgium). Numbers in dendrogram indicate percentage of similarity. Arrow numbers indicate molecular size (kb).
Mentions: S. suis was identified on the basis of colony morphology, negative katalase reaction, optochin resistance, and APIStrep (bioMérieux, Marcy l’Etoile, France). Serotyping was performed by slide agglutination using specific antisera (Statens Serum Institute, Copenhagen, Denmark) and was positive for serotype 16. Confirmation of the serotype was performed at the International Reference Laboratory for S. suis Serotyping, Quebec City, Quebec, Canada (M. Gottschalk). This strain was susceptible to penicillin (MIC 0.032 mg/L), ceftriaxone (0.064 mg/L), rifampin (0.032 mg/L), chloramphenicol (2 mg/L), erythromycin (0.064 mg/L), levofloxacin (0.38 mg/L), and vancomycin (0.5 mg/L) but resistant to tetracycline (64 mg/L) by Etest (AB-Biodisk, Solna, Sweden) when Clinical Laboratory Standard Institute breakpoints were used. On pulsed-field gel electrophoresis (PFGE) that used restriction enzyme SmaI, this strain showed little similarity with a representative set of serotype 2 isolates from Vietnam (Figure). Multilocus sequence typing (MLST) (www.mlst.net) showed that the sequence of 5 of 7 alleles of the included housekeeping genes had not been previously described. Thus, this strain was assigned the new sequence type 106. On eBURST analysis (www.mlst.net), this sequence type does not belong to any of the clonal complexes but is a singleton. PCR for detection of the genes encoding the putative virulence factors extracellular protein factor (EF) and suilysin were negative. Results of Western blot for detection of muramidase-released protein (MRP) and EF, using rabbit polyclonal antibody against MRP and EF (provided by H. Smith, the Netherlands), were also negative. S. suis serotype 2 strains 31533 and 89-1591 (provided by M. Gottschalk, Canada) were used as positive and negative controls, respectively.

Bottom Line: Streptococcus suis infection is an emerging zoonosis in Southeast Asia.We report a fatal case of S. suis serotype 16 infection in a Vietnamese man in 2001.

View Article: PubMed Central - PubMed

Affiliation: Oxford University Clinical Research Unit, The Hospital for Tropical Diseases, 190 Ben Ham Tu, Quan 5, Ho Chi Minh City, Vietnam.

ABSTRACT
Streptococcus suis infection is an emerging zoonosis in Southeast Asia. We report a fatal case of S. suis serotype 16 infection in a Vietnamese man in 2001.

Show MeSH
Related in: MedlinePlus