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Prolonged Bartonella henselae bacteremia caused by reinfection in cats.

Arvand M, Viezens J, Berghoff J - Emerging Infect. Dis. (2008)

Bottom Line: We analyzed the genetic relatedness of blood culture isolates of Bartonella henselae from 2 cats of patients with cat-scratch disease at admission and after 12 months.Isolates from each cat at different times were clonally unrelated, which suggested reinfection by a second strain.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Microbiology, Virology and Hygiene, University of Rostock, Schillingallee 70, Rostock, Germany. mardjan.arvand@med.uni-rostock.de

ABSTRACT
We analyzed the genetic relatedness of blood culture isolates of Bartonella henselae from 2 cats of patients with cat-scratch disease at admission and after 12 months. Isolates from each cat at different times were clonally unrelated, which suggested reinfection by a second strain.

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SmaI macrorestriction patterns of Bartonella henselae isolates from 2 cats. Lane 1, cat 36, first isolate; lane 2, cat 36, second isolate obtained 12 months later; lane 3, cat 75, first isolate; lane 4, cat 75, second isolate obtained 12 months later; lane 5, bacteriophage λ molecular mass pulsed-field gel electrophoresis marker. Values on the right are in kilobases.
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Figure 1: SmaI macrorestriction patterns of Bartonella henselae isolates from 2 cats. Lane 1, cat 36, first isolate; lane 2, cat 36, second isolate obtained 12 months later; lane 3, cat 75, first isolate; lane 4, cat 75, second isolate obtained 12 months later; lane 5, bacteriophage λ molecular mass pulsed-field gel electrophoresis marker. Values on the right are in kilobases.

Mentions: We tested 4 isolates of B. henselae: FR96/BK36, FR96/BK36II, FR96/BK75, and FR96/BK75II. These isolates were grown from the blood of 2 naturally infected cats (cat 36 and cat 75) of CSD patients at first consultation and after 12 months, respectively (9). The original colony counts were 100, 100, 120, and 100, respectively. PFGE analysis was conducted after digestion of DNA with SmaI, and MLST was conducted after partial sequencing of 8 genetic loci (12,13). PFGE analysis showed 9 band differences between isolates 36 and 36II and 10 band differences between isolates 75 and 75II (Figure), which suggested that isolates obtained from the same cat at different times were not clonally related (14). MLST analysis showed 3 and 6 different alleles between isolates 36 and 36II and isolates 75 and 75II, respectively (Table). Isolates 36 and 36II were assigned to sequence type (ST) 14 and ST5, which have the 16S rRNA alleles 1 and 2, respectively. Isolates 75 and 75II were assigned to ST5 and ST7, respectively, and both had the 16S rRNA allele 2 (Table). These data suggest that both cats were infected by a second B. henselae strain at the second time blood was obtained.


Prolonged Bartonella henselae bacteremia caused by reinfection in cats.

Arvand M, Viezens J, Berghoff J - Emerging Infect. Dis. (2008)

SmaI macrorestriction patterns of Bartonella henselae isolates from 2 cats. Lane 1, cat 36, first isolate; lane 2, cat 36, second isolate obtained 12 months later; lane 3, cat 75, first isolate; lane 4, cat 75, second isolate obtained 12 months later; lane 5, bacteriophage λ molecular mass pulsed-field gel electrophoresis marker. Values on the right are in kilobases.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2600141&req=5

Figure 1: SmaI macrorestriction patterns of Bartonella henselae isolates from 2 cats. Lane 1, cat 36, first isolate; lane 2, cat 36, second isolate obtained 12 months later; lane 3, cat 75, first isolate; lane 4, cat 75, second isolate obtained 12 months later; lane 5, bacteriophage λ molecular mass pulsed-field gel electrophoresis marker. Values on the right are in kilobases.
Mentions: We tested 4 isolates of B. henselae: FR96/BK36, FR96/BK36II, FR96/BK75, and FR96/BK75II. These isolates were grown from the blood of 2 naturally infected cats (cat 36 and cat 75) of CSD patients at first consultation and after 12 months, respectively (9). The original colony counts were 100, 100, 120, and 100, respectively. PFGE analysis was conducted after digestion of DNA with SmaI, and MLST was conducted after partial sequencing of 8 genetic loci (12,13). PFGE analysis showed 9 band differences between isolates 36 and 36II and 10 band differences between isolates 75 and 75II (Figure), which suggested that isolates obtained from the same cat at different times were not clonally related (14). MLST analysis showed 3 and 6 different alleles between isolates 36 and 36II and isolates 75 and 75II, respectively (Table). Isolates 36 and 36II were assigned to sequence type (ST) 14 and ST5, which have the 16S rRNA alleles 1 and 2, respectively. Isolates 75 and 75II were assigned to ST5 and ST7, respectively, and both had the 16S rRNA allele 2 (Table). These data suggest that both cats were infected by a second B. henselae strain at the second time blood was obtained.

Bottom Line: We analyzed the genetic relatedness of blood culture isolates of Bartonella henselae from 2 cats of patients with cat-scratch disease at admission and after 12 months.Isolates from each cat at different times were clonally unrelated, which suggested reinfection by a second strain.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Microbiology, Virology and Hygiene, University of Rostock, Schillingallee 70, Rostock, Germany. mardjan.arvand@med.uni-rostock.de

ABSTRACT
We analyzed the genetic relatedness of blood culture isolates of Bartonella henselae from 2 cats of patients with cat-scratch disease at admission and after 12 months. Isolates from each cat at different times were clonally unrelated, which suggested reinfection by a second strain.

Show MeSH
Related in: MedlinePlus