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Release of sICAM-1 in oocytes and in vitro fertilized human embryos.

Borgatti M, Rizzo R, Canto MB, Fumagalli D, Renzini MM, Fadini R, Stignani M, Baricordi OR, Gambari R - PLoS ONE (2008)

Bottom Line: No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics.On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine.

View Article: PubMed Central - PubMed

Affiliation: BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy.

ABSTRACT

Background: During the last years, several studies have reported the significant relationship between the production of soluble HLA-G molecules (sHLA-G) by 48-72 hours early embryos and an increased implantation rate in IVF protocols. As consequence, the detection of HLA-G modulation was suggested as a marker to identify the best embryos to be transferred. On the opposite, no suitable markers are available for the oocyte selection.

Methodology/principal findings: The major finding of the present paper is that the release of ICAM-1 might be predictive of oocyte maturation. The results obtained are confirmed using three independent methodologies, such as ELISA, Bio-Plex assay and Western blotting. The sICAM-1 release is very high in immature oocytes, decrease in mature oocytes and become even lower in in vitro fertilized embryos. No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics. On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.

Conclusions/significance: The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine. We propose sICAM-1 as a biochemical marker for oocyte maturation and grading, with a possible interesting rebound in assisted reproduction techniques.

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Related in: MedlinePlus

sICAM-1 levels in culture supernatants from immature (a) and mature oocytes (b).Immature oocytes were analysed individually for morphological characteristics to differentiate them as Metaphase I (MI), germinal vescicle (GV) and degenerated (DEG). (a). Mature oocytes were subdivided on the basis of the first polar body and cytoplasm characteristic in Grade 1, 2 and 3 (b). Preparation of oocyte supernatants was performed as described in the legend to Figure 2. * Student t Test.
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pone-0003970-g004: sICAM-1 levels in culture supernatants from immature (a) and mature oocytes (b).Immature oocytes were analysed individually for morphological characteristics to differentiate them as Metaphase I (MI), germinal vescicle (GV) and degenerated (DEG). (a). Mature oocytes were subdivided on the basis of the first polar body and cytoplasm characteristic in Grade 1, 2 and 3 (b). Preparation of oocyte supernatants was performed as described in the legend to Figure 2. * Student t Test.

Mentions: Figure 4a reports the levels of sICAM-1 in immature oocytes at different maturation stages (MI, Metaphase I; GV, germinal vesicle; DEG, degenerated). The average released sICAM-1 was 5900 pg/ml/24 hours for MI oocytes, 6600 pg/ml/24 hours for GV oocytes and 6600 pg/ml/24 hour for DEG oocytes. The difference between sICAM-1 production by MI, GV and DEG immature oocytes was not statistically significant (Student t Test, p = NS) (Figure 3a).


Release of sICAM-1 in oocytes and in vitro fertilized human embryos.

Borgatti M, Rizzo R, Canto MB, Fumagalli D, Renzini MM, Fadini R, Stignani M, Baricordi OR, Gambari R - PLoS ONE (2008)

sICAM-1 levels in culture supernatants from immature (a) and mature oocytes (b).Immature oocytes were analysed individually for morphological characteristics to differentiate them as Metaphase I (MI), germinal vescicle (GV) and degenerated (DEG). (a). Mature oocytes were subdivided on the basis of the first polar body and cytoplasm characteristic in Grade 1, 2 and 3 (b). Preparation of oocyte supernatants was performed as described in the legend to Figure 2. * Student t Test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2599884&req=5

pone-0003970-g004: sICAM-1 levels in culture supernatants from immature (a) and mature oocytes (b).Immature oocytes were analysed individually for morphological characteristics to differentiate them as Metaphase I (MI), germinal vescicle (GV) and degenerated (DEG). (a). Mature oocytes were subdivided on the basis of the first polar body and cytoplasm characteristic in Grade 1, 2 and 3 (b). Preparation of oocyte supernatants was performed as described in the legend to Figure 2. * Student t Test.
Mentions: Figure 4a reports the levels of sICAM-1 in immature oocytes at different maturation stages (MI, Metaphase I; GV, germinal vesicle; DEG, degenerated). The average released sICAM-1 was 5900 pg/ml/24 hours for MI oocytes, 6600 pg/ml/24 hours for GV oocytes and 6600 pg/ml/24 hour for DEG oocytes. The difference between sICAM-1 production by MI, GV and DEG immature oocytes was not statistically significant (Student t Test, p = NS) (Figure 3a).

Bottom Line: No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics.On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine.

View Article: PubMed Central - PubMed

Affiliation: BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy.

ABSTRACT

Background: During the last years, several studies have reported the significant relationship between the production of soluble HLA-G molecules (sHLA-G) by 48-72 hours early embryos and an increased implantation rate in IVF protocols. As consequence, the detection of HLA-G modulation was suggested as a marker to identify the best embryos to be transferred. On the opposite, no suitable markers are available for the oocyte selection.

Methodology/principal findings: The major finding of the present paper is that the release of ICAM-1 might be predictive of oocyte maturation. The results obtained are confirmed using three independent methodologies, such as ELISA, Bio-Plex assay and Western blotting. The sICAM-1 release is very high in immature oocytes, decrease in mature oocytes and become even lower in in vitro fertilized embryos. No significant differences were observed in the levels of sICAM-1 release between immature oocytes with different morphological characteristics. On the contrary, when the mature oocytes were subdivided accordingly to morphological criteria, the mean sICAM-I levels in grade 1 oocytes were significantly decreased when compared to grade 2 and 3 oocytes.

Conclusions/significance: The reduction of the number of fertilized oocytes and transferred embryos represents the main target of assisted reproductive medicine. We propose sICAM-1 as a biochemical marker for oocyte maturation and grading, with a possible interesting rebound in assisted reproduction techniques.

Show MeSH
Related in: MedlinePlus